FastQCFastQC Report
Mon 6 Feb 2017
SRR2077526_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077526_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5576142
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85600.034.845821
GGTATCA43550.033.9286271
TCAACGC110650.026.723794
ATCAACG112000.026.4315263
TATCAAC112750.026.3769262
CAACGCA112700.026.116165
AACGCAG119850.024.563956
GTACATG134950.020.5680871
ACGCAGA143050.020.3652697
CGCAGAG146150.019.7227618
TACATGG136850.019.488042
AGAGTAC141800.019.1128811
ACATGGG142850.018.1872793
GAGTACT86350.018.00918212
CAGAGTA156850.017.52062810
GCAGAGT163900.017.0612169
GTGTAGC26750.016.7776181
GTACTTT94150.016.70210314
AGTACTT95300.016.53644413
CATGGGG101900.015.7113094
Produced by FastQC (version 0.11.5)