FastQCFastQC Report
Mon 6 Feb 2017
SRR2077526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5576142
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50450.037.845321
GTATCAA100950.034.6058351
TATCAAC136450.025.485962
ATCAACG137600.024.9999873
TCAACGC138950.024.7813034
CAACGCA139600.024.5854455
AACGCAG144500.023.7927066
ACGCAGA173200.019.752457
GTACATG124750.019.1312271
CGCAGAG178550.019.064788
AGAGTAC175150.018.64726811
CGAATCG5000.018.62395937
GAGTACT125750.018.47741712
TACATGG127250.018.255262
AGTACTT135850.017.45368213
CAGAGTA191000.017.33482610
GTACTTT135600.017.19862714
GCAGAGT197350.016.9337439
ACATGGG132200.016.7633823
GTGGTAT27100.016.4647351
Produced by FastQC (version 0.11.5)