FastQCFastQC Report
Mon 6 Feb 2017
SRR2077525_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077525_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864120
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77850.035.6498071
GGTATCA37850.033.3791241
TCAACGC100000.027.1841354
ATCAACG100000.027.0109463
TATCAAC101750.026.6788852
CAACGCA102300.026.4724795
AACGCAG107300.025.3176276
GTACATG135850.021.979421
ACGCAGA125800.021.3212347
TACATGG137900.021.0858272
CGCAGAG130000.020.5544348
ACATGGG139050.020.5169373
AGAGTAC127850.019.74200411
GAGTACT70100.019.47886812
CAGAGTA138050.018.5074210
GCAGAGT140850.018.3598219
GTGGTAT20900.017.672471
GTACTTT75800.017.60582714
AGTACTT77400.017.42009513
CATGGGG68650.017.2362544
Produced by FastQC (version 0.11.5)