##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077525_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3864120
Sequences flagged as poor quality	0
Sequence length	75
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.982816009854766	31.0	31.0	34.0	26.0	34.0
2	30.027611720132914	31.0	31.0	34.0	26.0	34.0
3	30.154828266202912	31.0	31.0	34.0	26.0	34.0
4	33.586000434769105	37.0	35.0	37.0	28.0	37.0
5	33.61911301926441	37.0	35.0	37.0	28.0	37.0
6	33.63844989286047	37.0	35.0	37.0	28.0	37.0
7	33.55226571638562	37.0	35.0	37.0	28.0	37.0
8	33.45147303913957	37.0	35.0	37.0	27.0	37.0
9	34.88922316077141	39.0	35.0	39.0	27.0	39.0
10	34.82126098568367	39.0	35.0	39.0	27.0	39.0
11	34.83766989638003	39.0	35.0	39.0	27.0	39.0
12	34.75704455348178	39.0	35.0	39.0	27.0	39.0
13	34.724307216132004	39.0	35.0	39.0	27.0	39.0
14	35.86482070950178	40.0	36.0	41.0	27.0	41.0
15	35.88764324089314	40.0	36.0	41.0	27.0	41.0
16	35.87305699615954	40.0	36.0	41.0	27.0	41.0
17	35.798458898791964	40.0	36.0	41.0	26.0	41.0
18	35.761640166454455	40.0	36.0	41.0	26.0	41.0
19	35.85274965580779	40.0	36.0	41.0	26.0	41.0
20	35.83972676831983	40.0	36.0	41.0	26.0	41.0
21	35.818684719936236	40.0	36.0	41.0	26.0	41.0
22	35.75337748310094	40.0	36.0	41.0	26.0	41.0
23	35.711240334151114	40.0	36.0	41.0	26.0	41.0
24	35.69187059408093	40.0	36.0	41.0	25.0	41.0
25	35.71782786248874	40.0	36.0	41.0	26.0	41.0
26	35.660347763527014	40.0	36.0	41.0	25.0	41.0
27	35.6591573243067	40.0	36.0	41.0	26.0	41.0
28	35.62703099282631	40.0	36.0	41.0	25.0	41.0
29	35.601871318696105	40.0	36.0	41.0	25.0	41.0
30	35.52284789292258	40.0	36.0	41.0	25.0	41.0
31	35.505211018291355	40.0	36.0	41.0	25.0	41.0
32	35.4450143887871	40.0	35.0	41.0	25.0	41.0
33	35.33904355972382	40.0	35.0	41.0	25.0	41.0
34	35.309883233439955	40.0	35.0	41.0	25.0	41.0
35	35.26270198648075	40.0	35.0	41.0	25.0	41.0
36	35.18805471879755	40.0	35.0	41.0	24.0	41.0
37	35.11615115472604	40.0	35.0	41.0	24.0	41.0
38	35.03483095763072	40.0	35.0	41.0	24.0	41.0
39	34.9983395960788	40.0	35.0	41.0	24.0	41.0
40	34.95749303851847	40.0	35.0	41.0	24.0	41.0
41	34.88175988323344	40.0	35.0	41.0	23.0	41.0
42	34.79285089489974	40.0	35.0	41.0	22.0	41.0
43	34.69830077740857	39.0	35.0	41.0	22.0	41.0
44	34.59864471082678	39.0	35.0	41.0	21.0	41.0
45	34.49872804157221	39.0	34.0	41.0	20.0	41.0
46	34.4015460182396	39.0	34.0	41.0	19.0	41.0
47	34.29653038725505	39.0	34.0	41.0	18.0	41.0
48	34.190395743403414	39.0	34.0	41.0	16.0	41.0
49	34.094794933904744	39.0	34.0	41.0	15.0	41.0
50	33.97445964411043	39.0	33.0	41.0	14.0	41.0
51	33.8787470368415	39.0	33.0	41.0	13.0	41.0
52	33.78126378062793	39.0	33.0	41.0	12.0	41.0
53	33.70135088972392	39.0	33.0	40.0	9.0	41.0
54	33.71041815471569	39.0	33.0	41.0	9.0	41.0
55	33.620040267900585	38.0	33.0	41.0	8.0	41.0
56	33.488913128991854	38.0	33.0	40.0	8.0	41.0
57	33.402617931120155	38.0	33.0	40.0	7.0	41.0
58	33.31439448050268	38.0	33.0	40.0	6.0	41.0
59	33.20562379015144	38.0	33.0	40.0	2.0	41.0
60	33.18727549869052	38.0	33.0	40.0	2.0	41.0
61	33.086206173721315	38.0	33.0	40.0	2.0	41.0
62	32.942332536256636	38.0	33.0	40.0	2.0	41.0
63	32.75517064687432	37.0	32.0	40.0	2.0	41.0
64	32.617773516350425	37.0	32.0	40.0	2.0	41.0
65	32.46538358022008	37.0	32.0	40.0	2.0	41.0
66	32.304292050971505	37.0	32.0	40.0	2.0	41.0
67	32.13821956875045	36.0	32.0	40.0	2.0	41.0
68	31.953083496371747	36.0	32.0	39.0	2.0	41.0
69	31.978741084645403	36.0	32.0	39.0	2.0	41.0
70	31.87973044315394	36.0	32.0	39.0	2.0	41.0
71	31.715882011945798	36.0	32.0	39.0	2.0	41.0
72	31.515277475854788	35.0	32.0	39.0	2.0	41.0
73	31.306121445503763	35.0	32.0	39.0	2.0	40.0
74	31.08515703446063	35.0	32.0	38.0	2.0	40.0
75	30.862075711934413	35.0	32.0	37.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6133405086200803
1101	2	0.616245914458041
1101	3	0.6188849769567462
1101	4	0.7533827523822509
1101	5	0.7416822662550331
1101	6	0.6121039710289935
1101	7	0.6371029937524284
1101	8	0.6105997523263866
1101	9	0.7344437465941596
1101	10	0.8292063594239281
1101	11	0.7117591303033279
1101	12	0.7335226870972065
1101	13	0.7802673025731295
1101	14	0.7995458825491397
1101	15	0.8484064448051498
1101	16	0.7538160041984199
1101	17	0.8023202462167873
1101	18	0.8411757691210866
1101	19	0.8977842154780902
1101	20	0.8686362116030182
1101	21	0.8957067147071953
1101	22	0.9228609671976926
1101	23	0.9056216440034603
1101	24	0.808674567576773
1101	25	0.7637572676885753
1101	26	0.7774775922578812
1101	27	0.845304290885899
1101	28	0.754068636164746
1101	29	0.8556349009732074
1101	30	0.9272587241016268
1101	31	0.9656961491062575
1101	32	0.9484202767779948
1101	33	0.9214382103994083
1101	34	0.8317068204272147
1101	35	0.826727820176572
1101	36	0.9747896875626978
1101	37	0.9212707001228821
1101	38	0.8672319468828675
1101	39	0.7982476494276298
1101	40	0.9060049157232513
1101	41	0.9151736760666154
1101	42	0.7506816873212756
1101	43	0.7455913248792427
1101	44	0.8064826214351015
1101	45	0.7185657301595825
1101	46	0.8845493033561382
1101	47	0.8025971322784144
1101	48	0.8014657666761096
1101	49	0.797006774751658
1101	50	0.780203960010482
1101	51	0.7807003843090712
1101	52	0.7871908659340221
1101	53	0.7585032834837762
1101	54	0.7724993563475664
1101	55	0.6979114353993836
1101	56	0.6994479887400615
1101	57	0.6656040354392019
1101	58	0.660759708510767
1101	59	0.7375186872679365
1101	60	0.7215757057391485
1101	61	0.6906029334065948
1101	62	0.7459106158757507
1101	63	0.7557281797767956
1101	64	0.6970882214184257
1101	65	0.7184466964995124
1101	66	0.634352391917254
1101	67	0.6094201285014051
1101	68	0.6531983039933422
1101	69	0.6669700734134807
1101	70	0.7288652076870612
1101	71	0.6419951526500114
1101	72	0.666782459933863
1101	73	0.6772036392849472
1101	74	0.7047404719366419
1101	75	0.75812545017126
1102	1	0.734310171576869
1102	2	0.6877140532823169
1102	3	0.7289420301774143
1102	4	0.7584971285415207
1102	5	0.6657412088427961
1102	6	0.755953826527886
1102	7	0.7156939323592937
1102	8	0.7442643785277525
1102	9	0.9088917522305024
1102	10	0.924278327313111
1102	11	0.9456766486562387
1102	12	0.9732605281301119
1102	13	0.8657358144786116
1102	14	1.042266532487929
1102	15	1.0053013196745937
1102	16	1.0207516774541574
1102	17	1.0087263482004971
1102	18	0.9763366512662728
1102	19	0.966127058201522
1102	20	0.9503091104854988
1102	21	1.0303780798633753
1102	22	1.0851029025924959
1102	23	1.0836355110307423
1102	24	1.0308459557424356
1102	25	1.0154465302909799
1102	26	0.9726369400215731
1102	27	1.0162125177446057
1102	28	0.9525160779673669
1102	29	1.014760868857337
1102	30	1.0770805308698357
1102	31	1.0838687637787316
1102	32	1.0655197291897522
1102	33	1.0186753471861465
1102	34	1.0151048751447291
1102	35	1.0645111499734625
1102	36	1.0802248767457243
1102	37	1.0734005997737697
1102	38	1.0643738228523674
1102	39	1.0603343277547168
1102	40	1.0054499020475092
1102	41	1.0498829483701257
1102	42	0.96884028414906
1102	43	0.9224052980964572
1102	44	0.9824453218005047
1102	45	0.9857619799540132
1102	46	1.0162012628296253
1102	47	1.0219874462004483
1102	48	0.9693675979594758
1102	49	0.957630433747326
1102	50	0.9494824603276015
1102	51	0.9872363689712458
1102	52	0.9754585695156024
1102	53	0.8903379460476089
1102	54	0.9477654387824117
1102	55	0.9933522226724918
1102	56	0.9447247347830512
1102	57	0.894978748776154
1102	58	0.8726861207228183
1102	59	0.7972576995224898
1102	60	0.8519601001247352
1102	61	0.9289312837440136
1102	62	0.9262941320010967
1102	63	0.8803654450360057
1102	64	0.8054009842744634
1102	65	0.9127905258071536
1102	66	0.8980628065206702
1102	67	0.8988899940820403
1102	68	0.9123908418200699
1102	69	0.9538960106162193
1102	70	0.9139680544576727
1102	71	0.9265576182157389
1102	72	0.9220157132191602
1102	73	0.8877431490934278
1102	74	0.886866032077144
1102	75	0.8356390770107431
1103	1	0.6330754753804193
1103	2	0.6442379890961938
1103	3	0.6868770405213169
1103	4	0.6685541395780348
1103	5	0.6407744448648742
1103	6	0.6188016418765869
1103	7	0.7172475741158522
1103	8	0.686219206134929
1103	9	0.7764068045745987
1103	10	0.8625594314152139
1103	11	0.8028067584207363
1103	12	0.8444603121075573
1103	13	0.7677220427716946
1103	14	0.8379533982623215
1103	15	0.8756011293386692
1103	16	0.8994982811998042
1103	17	0.9282249231713635
1103	18	0.8725333818342236
1103	19	0.8937903439668773
1103	20	0.885668394105231
1103	21	0.8065878275430194
1103	22	0.8223292784387723
1103	23	0.8081781001179849
1103	24	0.8944894673198007
1103	25	0.8689333085567341
1103	26	0.9101056861356369
1103	27	0.9211625649225113
1103	28	0.913048438230021
1103	29	0.7912178507223189
1103	30	0.8429906190506244
1103	31	0.8469820096291514
1103	32	0.8244621507859264
1103	33	0.810678408642346
1103	34	0.8910189498832821
1103	35	0.8743645196108858
1103	36	0.8403172887860677
1103	37	0.8800446812311691
1103	38	0.8491977898689029
1103	39	0.9426852559358636
1103	40	1.004978736209246
1103	41	0.9499808937897356
1103	42	0.9650125066749027
1103	43	0.8691230257268217
1103	44	0.8776848238226975
1103	45	0.7583791292478352
1103	46	0.8394524219810435
1103	47	0.861413165310168
1103	48	0.8469617583628022
1103	49	0.8723210591265698
1103	50	0.8997263786134297
1103	51	0.9317638679487317
1103	52	0.9139457170621839
1103	53	0.8992253344807537
1103	54	0.8481276690208261
1103	55	0.8488233955403999
1103	56	0.8784197569223977
1103	57	0.7957032002789646
1103	58	0.7847667428184693
1103	59	0.756775367613244
1103	60	0.7752594108633488
1103	61	0.8641512307835484
1103	62	0.8719403320146597
1103	63	0.7998267778337649
1103	64	0.8099749812268229
1103	65	0.7719606989964234
1103	66	0.8080924468034567
1103	67	0.7469923916912649
1103	68	0.7621267200292507
1103	69	0.746219354761152
1103	70	0.801975664903221
1103	71	0.7692421321693317
1103	72	0.8123972803715986
1103	73	0.7815538678575003
1103	74	0.8150840916115527
1103	75	0.8605101181340906
1104	1	0.5447690300246428
1104	2	0.5483666194317713
1104	3	0.6205312782224475
1104	4	0.5927176171894075
1104	5	0.5429107845429755
1104	6	0.6206200941921267
1104	7	0.5834093856250178
1104	8	0.6332212403348478
1104	9	0.6877574705164946
1104	10	0.6575568675376289
1104	11	0.7086493130892464
1104	12	0.636434743593874
1104	13	0.5751433391713476
1104	14	0.6876015479583941
1104	15	0.7542341617617865
1104	16	0.6935142676356207
1104	17	0.7903164311899928
1104	18	0.8811719100525437
1104	19	0.772778552322066
1104	20	0.7329950361478268
1104	21	0.6976322599540552
1104	22	0.7197062779330352
1104	23	0.7199456954165555
1104	24	0.7320781336713864
1104	25	0.6814136554788774
1104	26	0.8007552542856615
1104	27	0.715624756626255
1104	28	0.7125407506641608
1104	29	0.7229787878422158
1104	30	0.7824630907814907
1104	31	0.732894619966892
1104	32	0.6824395423985692
1104	33	0.7732338836098265
1104	34	0.8005316654643124
1104	35	0.8030540531005386
1104	36	0.7510177410657235
1104	37	0.8244366886247363
1104	38	0.897477171014792
1104	39	0.8886562877245439
1104	40	0.7926612325774514
1104	41	0.7635691392604329
1104	42	0.8103250237104547
1104	43	0.7948338578312928
1104	44	0.8566243101391962
1104	45	0.8687926083734752
1104	46	0.798992478090419
1104	47	0.7644128703589672
1104	48	0.8074312886000925
1104	49	0.7871974966098634
1104	50	0.744122631735209
1104	51	0.7655582031435486
1104	52	0.8271644745740474
1104	53	0.7829598747084816
1104	54	0.7918759236429125
1104	55	0.7572392595423452
1104	56	0.8066396644151368
1104	57	0.7012345073038944
1104	58	0.6693194221815943
1104	59	0.664356276857859
1104	60	0.7261914260826643
1104	61	0.8003527611955121
1104	62	0.7396068388319605
1104	63	0.7288685452427401
1104	64	0.7275257371481558
1104	65	0.7341770637289358
1104	66	0.7580398107340685
1104	67	0.7862101716472836
1104	68	0.7477619389447057
1104	69	0.743012676230677
1104	70	0.7185387698365666
1104	71	0.693403763811915
1104	72	0.6736591407931698
1104	73	0.7629787780871702
1104	74	0.6700081931626798
1104	75	0.7221153287501885
1105	1	0.39886145405738915
1105	2	0.41797759555809577
1105	3	0.45000106188032873
1105	4	0.33690894999294585
1105	5	0.3926596733039034
1105	6	0.4125509935577014
1105	7	0.4560712223973624
1105	8	0.4746174708092923
1105	9	0.4960299286078609
1105	10	0.47658491388559554
1105	11	0.5082417114342164
1105	12	0.4848818686321934
1105	13	0.48888634174450374
1105	14	0.543590628184468
1105	15	0.5514597925492595
1105	16	0.49822917564426206
1105	17	0.4826537461943161
1105	18	0.5178659406274875
1105	19	0.4973576370799435
1105	20	0.6024736384464688
1105	21	0.6182875760753319
1105	22	0.6230044396022407
1105	23	0.5936971985120891
1105	24	0.6831869397481327
1105	25	0.6007261129672656
1105	26	0.7044645036110566
1105	27	0.637497180400743
1105	28	0.5722954326158813
1105	29	0.6445709737018319
1105	30	0.712696125802168
1105	31	0.6013006811308585
1105	32	0.6113786003923849
1105	33	0.686102891934496
1105	34	0.6601269279831996
1105	35	0.6328597176806099
1105	36	0.6872562305420757
1105	37	0.5465743810150059
1105	38	0.6178959156808332
1105	39	0.5485726558411699
1105	40	0.5950572847615021
1105	41	0.5521877833146078
1105	42	0.5693881140687722
1105	43	0.49984350751477535
1105	44	0.6777935869746301
1105	45	0.6400448426147989
1105	46	0.6658310025819603
1105	47	0.6859773455575677
1105	48	0.6870988238481885
1105	49	0.6476717690068838
1105	50	0.6514243188655513
1105	51	0.7002813074115082
1105	52	0.6869273588814266
1105	53	0.6350520600970171
1105	54	0.5917889343358524
1105	55	0.6047154861505959
1105	56	0.5858810207810521
1105	57	0.5707786730265028
1105	58	0.5822457361532543
1105	59	0.4857676894492613
1105	60	0.4792070803338646
1105	61	0.531004193944753
1105	62	0.5103185489194786
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2213	4	-0.3090912468589977
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2214	1	-0.41101934753666214
2214	2	-0.38622457976205027
2214	3	-0.2932396320899464
2214	4	-0.33746377347233647
2214	5	-0.33190144253125453
2214	6	-0.27867584131341516
2214	7	-0.22514553543645377
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2214	11	-0.23082599762623346
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2214	15	-0.37457739349518704
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2214	21	-0.36918131075471194
2214	22	-0.32934041336693554
2214	23	-0.25064080105640585
2214	24	-0.3172157863823628
2214	25	-0.4273192971580002
2214	26	-0.3520435364432615
2214	27	-0.37805613017253137
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2214	29	-0.304847883303232
2214	30	-0.34380899521111985
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2214	46	-0.5009342938276191
2214	47	-0.3467634816732783
2214	48	-0.3906620939998433
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2214	51	-0.4553910746528871
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2214	53	-0.3026766721507741
2214	54	-0.3276712715200034
2214	55	-0.3894204650125772
2214	56	-0.30709563958217956
2214	57	-0.3326983109695334
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2214	60	-0.4768592961208924
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2214	75	-0.35543321004725925
2215	1	-0.8750874885418014
2215	2	-0.9339868980322024
2215	3	-1.03446498939973
2215	4	-1.1358955463684524
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2215	6	-0.9255590176190438
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2215	10	-1.0959187448188672
2215	11	-1.104606864025584
2215	12	-1.1418090010343533
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2215	15	-1.425859379178192
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2215	20	-1.2547063787826929
2215	21	-1.5301822209414269
2215	22	-1.4336833323603173
2215	23	-1.5436159308195059
2215	24	-1.3396992677235318
2215	25	-1.4672107143433095
2215	26	-1.4640539174916967
2215	27	-1.5210945794111765
2215	28	-1.5104553158640073
2215	29	-1.4593970948847428
2215	30	-1.5322039670445307
2215	31	-1.4415137720985314
2215	32	-1.5875022531324348
2215	33	-1.4416256637202594
2215	34	-1.4644874089253221
2215	35	-1.4078769101021607
2215	36	-1.5821545308636473
2215	37	-1.6009748370482129
2215	38	-1.4009062136358281
2215	39	-1.5419556987028997
2215	40	-1.5947054446201534
2215	41	-1.5871129206941532
2215	42	-1.5152797971316474
2215	43	-1.4318396938702236
2215	44	-1.4660938663850374
2215	45	-1.4826266888523847
2215	46	-1.4812012983293528
2215	47	-1.521154369966311
2215	48	-1.513984718289528
2215	49	-1.4330796650728317
2215	50	-1.4826844992387578
2215	51	-1.457923410880781
2215	52	-1.2833848074763026
2215	53	-1.383702477174019
2215	54	-1.4386698693404725
2215	55	-1.5033296431385779
2215	56	-1.3593287949781114
2215	57	-1.3094754136238365
2215	58	-1.4244928120899125
2215	59	-1.4554289349767373
2215	60	-1.529786259787226
2215	61	-1.4882961481111998
2215	62	-1.467184026300881
2215	63	-1.4141934053540943
2215	64	-1.441599103236161
2215	65	-1.4896323485663103
2215	66	-1.565672341671128
2215	67	-1.4879636214852034
2215	68	-1.5245448059008346
2215	69	-1.5189360084888328
2215	70	-1.512833044711357
2215	71	-1.4939121350004143
2215	72	-1.6638319257237235
2215	73	-1.6098535396963598
2215	74	-1.5525560368755436
2215	75	-1.6038303958483446
2216	1	-0.3135681274844053
2216	2	-0.13451145811110266
2216	3	-0.3308410964478874
2216	4	-0.1936651522125743
2216	5	-0.12228651552773329
2216	6	-0.20318116289645616
2216	7	-0.1656425314360419
2216	8	-0.251348956801813
2216	9	-0.40130637320264384
2216	10	-0.4339548676377021
2216	11	-0.31605012410425104
2216	12	-0.32029312084265626
2216	13	-0.4711567764660529
2216	14	-0.5778870082965071
2216	15	-0.5497032662790886
2216	16	-0.49399194886577646
2216	17	-0.4590348770906516
2216	18	-0.5475256005170408
2216	19	-0.5178658926767454
2216	20	-0.44467726335400926
2216	21	-0.7371745203852313
2216	22	-0.569700184856238
2216	23	-0.634554651933982
2216	24	-0.4654446513573447
2216	25	-0.518958551398292
2216	26	-0.485649399779696
2216	27	-0.5834752583481375
2216	28	-0.5359613102007259
2216	29	-0.6275150223434309
2216	30	-0.5788678500005489
2216	31	-0.524694894269004
2216	32	-0.5635634768469728
2216	33	-0.5528125658952305
2216	34	-0.6289016081325869
2216	35	-0.6257673201514535
2216	36	-0.5674285419482885
2216	37	-0.5334229062079601
2216	38	-0.5033648696104933
2216	39	-0.6081030094058164
2216	40	-0.6004819510718775
2216	41	-0.6021085662367156
2216	42	-0.3496013600207064
2216	43	-0.5019147000209614
2216	44	-0.5264949563771353
2216	45	-0.5873720563460694
2216	46	-0.5898934778023488
2216	47	-0.47519080257728774
2216	48	-0.6279203199420067
2216	49	-0.6078503295984419
2216	50	-0.5971549955573039
2216	51	-0.7299878005138609
2216	52	-0.4807893532340657
2216	53	-0.6128569121755731
2216	54	-0.594588473297307
2216	55	-0.6509522247515918
2216	56	-0.631589535630475
2216	57	-0.5631581075536829
2216	58	-0.6161533771156726
2216	59	-0.49285054262241346
2216	60	-0.6103337805694693
2216	61	-0.583756343166101
2216	62	-0.6487929165497164
2216	63	-0.6396985159731443
2216	64	-0.6369985917968322
2216	65	-0.6848345655684618
2216	66	-0.6357525642108719
2216	67	-0.6029919032121853
2216	68	-0.5625855682374272
2216	69	-0.49425162610591755
2216	70	-0.45112316271650954
2216	71	-0.4304931798578622
2216	72	-0.44082997608299124
2216	73	-0.5042833383615424
2216	74	-0.628674014902515
2216	75	-0.5828816844283757
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	141822.0
3	22256.0
4	15864.0
5	11504.0
6	9612.0
7	8465.0
8	7912.0
9	8304.0
10	8554.0
11	9282.0
12	10168.0
13	10504.0
14	11173.0
15	11650.0
16	12487.0
17	13079.0
18	13914.0
19	14760.0
20	16833.0
21	19262.0
22	24958.0
23	29953.0
24	32306.0
25	34523.0
26	36339.0
27	38536.0
28	45165.0
29	55168.0
30	61408.0
31	67761.0
32	82566.0
33	104262.0
34	134933.0
35	180519.0
36	253169.0
37	393785.0
38	658289.0
39	1042839.0
40	210236.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.518931902399764	18.302584786236324	13.195627756764802	23.982855554599112
2	15.561884382845626	20.133461614303553	39.83103395826688	24.473620044583942
3	19.165964671254965	25.176764215309916	28.863658800949516	26.793612312485603
4	12.468167005233752	16.197778467755438	37.99997822941941	33.334076297591395
5	13.55177428413479	38.00601963573765	34.43653581444821	14.005670265679344
6	31.806963690648775	37.067522339502396	17.54769305949937	13.577820910349459
7	28.149402789934097	32.18311643691867	21.598851234089036	18.068629539058197
8	25.959828263208856	35.403797503112436	19.919017202174118	18.717357031504594
9	26.248186340553424	16.305290703699864	19.438050575189138	38.00847238055757
10	14.029712533903215	29.836616819311025	33.90305934733785	22.23061129944791
11	34.5219347085993	23.85341737688443	23.36173513323985	18.262912781276423
12	23.107179962024002	25.614337066922914	30.25456669551018	21.023916275542902
13	27.64372347489903	22.704812528737957	25.973513648293554	23.67795034806946
14	21.515309388842667	21.89140222234318	27.570378088639714	29.022910300174438
15	23.20985369916705	29.87788870480238	23.97221027949101	22.940047316539562
16	22.934487812273638	29.017375495484753	25.587396448707366	22.46074024353424
17	21.708102796705564	28.76521852695646	26.802875894765428	22.723802781572548
18	21.690987558189402	28.31576260667787	27.819230325731088	22.174019509401646
19	22.616506037213497	27.86880401478586	28.073405672751406	21.441284275249238
20	22.856568249272	27.422838530301163	27.995336050083296	21.72525717034354
21	23.175616586203876	27.198427826353814	27.81938146724111	21.806574120201198
22	22.655248916626924	27.167497771050613	27.762316241265626	22.414937071056833
23	22.493656129970987	27.278835543092043	27.615697698683217	22.611810628253753
24	22.080605171200975	27.263936111371738	28.02643917626135	22.62901954116594
25	22.525345803715915	27.21480558927276	27.8501811914511	22.40966741556022
26	22.266948103605635	27.34240391143083	28.02237325163609	22.36827473332744
27	22.412482108585362	27.585716111567244	27.60702637105725	22.394775408790146
28	22.329872324363365	27.416443348586455	27.71099767120095	22.54268665584923
29	22.39204287382378	27.539851999029363	27.836019860668372	22.232085266478485
30	22.568569276971793	27.500398564390512	27.722894705120677	22.208137453517026
31	22.56986545276277	27.146141077181568	27.803413106605202	22.480580363450457
32	22.25996966149654	27.529807113062247	27.729480295007214	22.480742930433998
33	22.36786434266088	27.353983980288536	27.676727034403804	22.601424642646776
34	22.540202764021057	27.376772888739076	27.876733995384654	22.206290351855213
35	22.644871504034743	27.49019023236219	27.609994490715234	22.254943772887835
36	22.528809298621137	27.430979018368262	27.624129913551364	22.41608176945924
37	22.652241715601797	27.17350548403642	27.726784055661323	22.44746874470046
38	22.437723264233618	27.32174708046808	27.808847830151034	22.43168182514727
39	22.61156106164593	27.54576033230094	27.55682908089467	22.28584952515846
40	22.718626446342707	27.21514983052535	27.8406969411589	22.22552678197304
41	22.484219860224712	27.39473749029338	27.638300658170216	22.482741991311688
42	22.465703532839626	27.371361658723565	27.645738584187047	22.517196224249762
43	22.338558752973352	27.248601718572086	27.727553139102586	22.68528638935198
44	22.420533168369968	27.538424762342306	27.570833218823452	22.47020885046427
45	22.589315991583543	27.523719253635537	27.377966008538827	22.508998746242096
46	22.592592208390368	27.28857980588144	27.348356869849898	22.770471115878294
47	22.54930736588495	27.487381784446185	27.324082654562442	22.63922819510642
48	22.359048259319664	27.62938386265453	27.399738140590696	22.611829737435105
49	22.56206045133978	27.448509597215004	27.24364519602317	22.745784755422047
50	22.68146476365899	27.49449454460009	27.2990447182252	22.52499597351572
51	22.581550511540463	27.675892777010276	27.045500410093542	22.69705630135572
52	22.58029211372813	27.63404582207656	27.103913116433908	22.681748947761402
53	22.603992837424844	27.626560654330547	27.175385844189353	22.594060664055256
54	22.54219076698417	27.638115645016757	27.36801902525638	22.451674562742692
55	22.499117757410644	27.543159877914743	27.194748601700596	22.762973762974024
56	22.476992960111623	27.858870272323994	27.06862367523928	22.59551309232511
57	22.631219753024958	27.51774627494176	27.02282641840551	22.82820755362777
58	22.45637987383083	27.363428473315686	27.355466064186356	22.824725588667132
59	22.56232316220986	27.6615805861547	27.17437204751574	22.601724204119705
60	22.397674774520052	27.768312707030407	27.26539579934165	22.568616719107883
61	22.801711997103443	27.40735976163589	27.265488713341117	22.52543952791955
62	22.359468311337007	27.604526855507	27.27960139433126	22.756403438824726
63	22.370248103703965	27.48207482650798	27.39383699896948	22.753840070818576
64	22.488202726342678	27.415276483686146	27.353047680907096	22.743473109064084
65	22.36399484501978	27.462334628997358	27.32603952583938	22.84763100014348
66	22.43873795294077	27.60837522684484	27.147410435031144	22.805476385183248
67	22.50942199974681	27.489229035531732	27.100467779458796	22.900881185262666
68	22.523641486600955	27.53642508458857	27.32528434363266	22.61464908517782
69	22.64053519532731	27.566470207767864	26.99280953168853	22.800185065216297
70	22.38702224463129	27.269890771354365	27.335198680769857	23.007888303244492
71	22.480562253308094	27.424968962109002	27.19082642615446	22.903642358428446
72	22.37381543935724	27.562567105652175	27.04633985673754	23.01727759825305
73	22.46119997282196	27.357889697602783	27.233876911474788	22.947033418100474
74	22.36681256227014	27.493100688712307	27.109197646791568	23.030889102225984
75	22.513355334118113	27.52397267558561	27.0825994406246	22.880072549671674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2630.0
1	2431.0
2	2590.0
3	2863.5
4	2779.0
5	2410.5
6	1940.0
7	1956.5
8	2075.0
9	2569.0
10	3729.5
11	4486.5
12	4577.0
13	4453.5
14	4563.5
15	5051.5
16	5306.0
17	5745.0
18	6924.5
19	8585.5
20	9506.0
21	11023.0
22	14965.5
23	19925.5
24	22460.0
25	26176.5
26	34675.0
27	44268.5
28	49080.0
29	55354.5
30	67146.5
31	79184.0
32	85704.0
33	92133.0
34	105869.0
35	119986.5
36	126797.0
37	131182.5
38	139883.5
39	148162.5
40	152126.0
41	157387.5
42	165761.0
43	172149.5
44	175426.0
45	178457.0
46	182901.5
47	186445.0
48	188575.0
49	189024.0
50	186976.5
51	181007.5
52	177535.0
53	172198.5
54	160710.5
55	146722.0
56	138885.0
57	131719.0
58	115172.5
59	96124.5
60	86457.0
61	78517.5
62	62346.0
63	46628.5
64	39143.0
65	32496.5
66	22048.5
67	15731.5
68	13216.0
69	10963.5
70	7213.5
71	4632.5
72	3549.0
73	3126.5
74	2315.5
75	1512.0
76	1097.0
77	876.5
78	509.5
79	316.0
80	269.0
81	200.5
82	115.5
83	76.0
84	53.0
85	41.5
86	25.5
87	14.5
88	8.0
89	10.0
90	16.0
91	17.0
92	14.0
93	12.0
94	9.5
95	10.0
96	11.0
97	6.5
98	5.5
99	11.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18746829808598076
2	0.12636771114768694
3	0.12636771114768694
4	0.14756270509197436
5	0.14872726519880333
6	0.12165771249340082
7	0.12095897642930344
8	0.0981853565624256
9	0.11697359295259982
10	0.11914743848534726
11	0.1378580375350662
12	0.13889320207446976
13	0.15480885686779913
14	0.15605105431508337
15	0.14044594888357503
16	0.14298210200511371
17	0.12960260033332296
18	0.1282310073186133
19	0.16510874403486434
20	0.14461248615467429
21	0.15584402140720266
22	0.15009885821351304
23	0.16715319400018633
24	0.15284204424293243
25	0.17789302609649804
26	0.16301253584257216
27	0.17657319130875854
28	0.1751239609535936
29	0.17722016914588573
30	0.16886121549020217
31	0.17631440017390765
32	0.16345248077181868
33	0.17784126786952786
34	0.19202302205935634
35	0.18102439882819374
36	0.18198192602714203
37	0.19807873461486705
38	0.1921006593998116
39	0.16593687566638718
40	0.19398983468422304
41	0.1867178037949132
42	0.23819136051675413
43	0.2013136238005031
44	0.18379346397109822
45	0.17897994886287175
46	0.21029367617982878
47	0.19135016510874403
48	0.2229744417875221
49	0.22998768154198107
50	0.21735867416125795
51	0.23037586824425743
52	0.21461548813183853
53	0.20612713890872952
54	0.19026324234237033
55	0.19334285684709585
56	0.21311449954970343
57	0.1950249992236266
58	0.2200501019637071
59	0.23011707710940654
60	0.23317081250064697
61	0.2201277393041624
62	0.23503410867157332
63	0.2526836640684037
64	0.23270498845791537
65	0.258351189921638
66	0.22025713487158782
67	0.24098630477314367
68	0.21733279504777284
69	0.21174290653499372
70	0.22095587093568522
71	0.2160647184870035
72	0.21376147738683063
73	0.2087409293707235
74	0.20542840284463215
75	0.23565520739521548
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	3864120.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.09117968641001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.15717480176268	42.337205916439494
2	12.9256618936125	14.758825714680276
3	5.386388289453694	9.225457850819264
4	2.6481536200249662	6.047448566322499
5	1.466569416011617	4.18640890260563
6	0.9057396507207837	3.1025847089043896
7	0.5769326958228	2.3056437742929083
8	0.4141576921650162	1.8915800977521455
9	0.2811855254587022	1.4447892023262343
>10	1.1723750971067226	11.03865438325164
>50	0.048262009232795465	1.884119070647377
>100	0.017080932081635143	1.61948389524771
>500	2.2741182658443806E-4	0.09015540360796305
>1k	9.096471966744629E-5	0.06764251310244662
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.5879113485088454E-5	0.0
11	0.0	0.0	0.0	2.5879113485088454E-5	0.0
12	0.0	0.0	0.0	2.5879113485088454E-5	0.0
13	0.0	0.0	0.0	2.5879113485088454E-5	0.0
14	0.0	0.0	0.0	5.175822697017691E-5	0.0
15	0.0	0.0	0.0	5.175822697017691E-5	0.0
16	0.0	0.0	0.0	5.175822697017691E-5	0.0
17	0.0	0.0	0.0	7.763734045526537E-5	0.0
18	0.0	0.0	0.0	7.763734045526537E-5	0.0
19	0.0	0.0	0.0	7.763734045526537E-5	0.0
20	0.0	0.0	0.0	7.763734045526537E-5	0.0
21	0.0	0.0	0.0	1.2939556742544228E-4	0.0
22	0.0	0.0	0.0	1.5527468091053074E-4	0.0
23	0.0	0.0	0.0	2.84670248335973E-4	0.0
24	0.0	0.0	0.0	3.881867022763268E-4	0.0
25	0.0	0.0	0.0	4.1406581576141526E-4	0.0
26	0.0	0.0	0.0	6.210987236421229E-4	0.0
27	0.0	0.0	0.0	9.057689719780959E-4	0.0
28	0.0	0.0	0.0	0.0015268676956202189	0.0
29	0.0	0.0	0.0	0.002613790461993934	0.0
30	0.0	0.0	0.0	0.004088899930643976	0.0
31	0.0	0.0	0.0	0.008074283407347598	0.0
32	0.0	0.0	0.0	0.0138970839414925	0.0
33	2.5879113485088454E-5	0.0	0.0	0.019901038270033023	0.0
34	2.5879113485088454E-5	0.0	0.0	0.028881090649358714	0.0
35	2.5879113485088454E-5	0.0	0.0	0.038482241752326535	0.0
36	2.5879113485088454E-5	0.0	0.0	0.05061954597683302	0.0
37	2.5879113485088454E-5	0.0	0.0	0.06694926658592383	0.0
38	2.5879113485088454E-5	0.0	0.0	0.08754904092005424	0.0
39	2.5879113485088454E-5	0.0	0.0	0.1102709025599619	0.0
40	2.5879113485088454E-5	0.0	0.0	0.13312215976729502	0.0
41	2.5879113485088454E-5	0.0	0.0	0.1555593511588667	0.0
42	2.5879113485088454E-5	0.0	0.0	0.17711665269194538	0.0
43	2.5879113485088454E-5	0.0	0.0	0.201287744687018	0.0
44	2.5879113485088454E-5	0.0	0.0	0.22900427522954775	0.0
45	2.5879113485088454E-5	0.0	0.0	0.26233657339834165	0.0
46	2.5879113485088454E-5	0.0	0.0	0.29732513483018125	0.0
47	2.5879113485088454E-5	0.0	0.0	0.3345910582487086	0.0
48	2.5879113485088454E-5	0.0	0.0	0.37126176205707895	0.0
49	2.5879113485088454E-5	0.0	0.0	0.41008043228471164	0.0
50	2.5879113485088454E-5	0.0	0.0	0.44887322339885927	0.0
51	2.5879113485088454E-5	0.0	0.0	0.4913149695144043	0.0
52	2.5879113485088454E-5	0.0	0.0	0.531893419459023	0.0
53	2.5879113485088454E-5	0.0	0.0	0.5717472542260592	0.0
54	2.5879113485088454E-5	0.0	0.0	0.6156382306967693	0.0
55	2.5879113485088454E-5	0.0	0.0	0.6641098102543399	2.5879113485088454E-5
56	2.5879113485088454E-5	0.0	0.0	0.7173431466931669	2.5879113485088454E-5
57	2.5879113485088454E-5	0.0	0.0	0.7754158773537053	2.5879113485088454E-5
58	2.5879113485088454E-5	0.0	0.0	0.8375257497179177	2.5879113485088454E-5
59	2.5879113485088454E-5	0.0	0.0	0.8991697980393983	2.5879113485088454E-5
60	2.5879113485088454E-5	0.0	0.0	0.9652391747668292	2.5879113485088454E-5
61	2.5879113485088454E-5	0.0	0.0	1.0356044843327847	2.5879113485088454E-5
62	2.5879113485088454E-5	0.0	0.0	1.1051934204941876	2.5879113485088454E-5
63	2.5879113485088454E-5	0.0	0.0	1.1746529610881649	2.5879113485088454E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7785	0.0	35.649807	1
GGTATCA	3785	0.0	33.379124	1
TCAACGC	10000	0.0	27.184135	4
ATCAACG	10000	0.0	27.010946	3
TATCAAC	10175	0.0	26.678885	2
CAACGCA	10230	0.0	26.472479	5
AACGCAG	10730	0.0	25.317627	6
GTACATG	13585	0.0	21.97942	1
ACGCAGA	12580	0.0	21.321234	7
TACATGG	13790	0.0	21.085827	2
CGCAGAG	13000	0.0	20.554434	8
ACATGGG	13905	0.0	20.516937	3
AGAGTAC	12785	0.0	19.742004	11
GAGTACT	7010	0.0	19.478868	12
CAGAGTA	13805	0.0	18.50742	10
GCAGAGT	14085	0.0	18.359821	9
GTGGTAT	2090	0.0	17.67247	1
GTACTTT	7580	0.0	17.605827	14
AGTACTT	7740	0.0	17.420095	13
CATGGGG	6865	0.0	17.236254	4
>>END_MODULE