FastQCFastQC Report
Mon 6 Feb 2017
SRR2077525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864120
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43210.11182364936906722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50350.041.4288831
GTATCAA103550.034.976951
ATCAACG144600.025.0619533
TATCAAC144300.024.9673562
TCAACGC145900.024.8149434
CAACGCA148800.024.2248155
AACGCAG154700.023.3434436
CGCGTAA850.004102141620.3109810
GTGGTAT24450.020.090691
GTACATG123200.019.8515341
ACGCAGA181700.019.7794277
TACATGG120300.019.630272
CGCAGAG187250.019.2123578
GAGTACT130250.018.98082412
TGGTATC23850.018.84632
AGAGTAC183300.018.78073111
ACATGGG123850.018.734773
AGTACTT137800.017.98469513
CAGAGTA194350.017.96165810
CGTGCCG2902.1827873E-1117.85965510
Produced by FastQC (version 0.11.5)