##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077525_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3864120 Sequences flagged as poor quality 0 Sequence length 75 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.92152650538803 31.0 31.0 34.0 27.0 34.0 2 31.026828100576587 33.0 31.0 34.0 27.0 34.0 3 31.100310290570686 33.0 31.0 34.0 27.0 34.0 4 34.46679528586069 37.0 35.0 37.0 31.0 37.0 5 34.20514088589381 37.0 35.0 37.0 30.0 37.0 6 34.193518834818796 37.0 35.0 37.0 30.0 37.0 7 34.03410142542157 37.0 35.0 37.0 30.0 37.0 8 33.99092678281213 37.0 35.0 37.0 30.0 37.0 9 35.45749459126528 39.0 37.0 39.0 29.0 39.0 10 35.35496413154871 39.0 35.0 39.0 29.0 39.0 11 35.34782434292931 39.0 35.0 39.0 29.0 39.0 12 35.24607543243999 39.0 35.0 39.0 28.0 39.0 13 35.17816242766788 39.0 35.0 39.0 27.0 39.0 14 36.41925871867333 40.0 37.0 41.0 27.0 41.0 15 36.460885790296366 40.0 37.0 41.0 28.0 41.0 16 36.44655367845719 40.0 37.0 41.0 28.0 41.0 17 36.4554058880159 40.0 37.0 41.0 28.0 41.0 18 36.465294556069686 40.0 37.0 41.0 28.0 41.0 19 36.511562529114 40.0 37.0 41.0 28.0 41.0 20 36.48441533906814 40.0 37.0 41.0 28.0 41.0 21 36.45707276171547 40.0 37.0 41.0 28.0 41.0 22 36.441286243698435 40.0 37.0 41.0 28.0 41.0 23 36.415591130710226 40.0 37.0 41.0 28.0 41.0 24 36.39954401002039 40.0 37.0 41.0 28.0 41.0 25 36.374455503452275 40.0 37.0 41.0 28.0 41.0 26 36.30602077575231 40.0 37.0 41.0 28.0 41.0 27 36.250426487790236 40.0 36.0 41.0 27.0 41.0 28 36.231199859217625 40.0 36.0 41.0 28.0 41.0 29 36.17724164881008 40.0 36.0 41.0 28.0 41.0 30 36.101843369253544 40.0 36.0 41.0 27.0 41.0 31 36.002188596627434 40.0 36.0 41.0 27.0 41.0 32 35.92921363725764 40.0 36.0 41.0 27.0 41.0 33 35.86156977526578 40.0 35.0 41.0 27.0 41.0 34 35.79676821630798 40.0 35.0 41.0 27.0 41.0 35 35.7154839911804 40.0 35.0 41.0 26.0 41.0 36 35.65727513638293 40.0 35.0 41.0 26.0 41.0 37 35.59767709077358 40.0 35.0 41.0 26.0 41.0 38 35.5376139974949 40.0 35.0 41.0 25.0 41.0 39 35.46725179342256 40.0 35.0 41.0 25.0 41.0 40 35.39753682597849 40.0 35.0 41.0 25.0 41.0 41 35.34711240851733 40.0 35.0 41.0 25.0 41.0 42 35.28404914961233 40.0 35.0 41.0 25.0 41.0 43 35.18528254816103 40.0 35.0 41.0 24.0 41.0 44 35.056885655724976 40.0 35.0 41.0 24.0 41.0 45 34.9561908015279 40.0 35.0 41.0 23.0 41.0 46 34.81371282465348 39.0 35.0 41.0 23.0 41.0 47 34.65744490336739 39.0 35.0 41.0 22.0 41.0 48 34.54462568450255 39.0 34.0 41.0 20.0 41.0 49 34.45168317754107 39.0 34.0 41.0 19.0 41.0 50 34.44929686448661 39.0 34.0 41.0 19.0 41.0 51 34.380488442387914 39.0 34.0 41.0 18.0 41.0 52 34.321169632413074 39.0 34.0 41.0 18.0 41.0 53 34.22804571286606 39.0 34.0 41.0 17.0 41.0 54 34.20292641015289 39.0 34.0 41.0 16.0 41.0 55 34.12673286543896 39.0 34.0 41.0 15.0 41.0 56 34.01355677359916 39.0 34.0 41.0 14.0 41.0 57 33.94367152158836 39.0 34.0 41.0 13.0 41.0 58 33.8508591865677 39.0 34.0 41.0 13.0 41.0 59 33.762935933666654 38.0 34.0 41.0 12.0 41.0 60 33.735384770659294 38.0 34.0 41.0 10.0 41.0 61 33.631469519580136 38.0 34.0 41.0 8.0 41.0 62 33.49231726757968 38.0 34.0 41.0 8.0 41.0 63 33.36539910768817 38.0 34.0 40.0 7.0 41.0 64 33.219631119116386 37.0 33.0 40.0 2.0 41.0 65 33.09700863327226 37.0 33.0 40.0 2.0 41.0 66 32.90575292692773 37.0 33.0 40.0 2.0 41.0 67 32.720509456228065 37.0 33.0 40.0 2.0 41.0 68 32.51305342484188 36.0 33.0 39.0 2.0 41.0 69 32.346943676697414 36.0 33.0 39.0 2.0 41.0 70 32.15807531857189 36.0 33.0 39.0 2.0 41.0 71 31.981324855335757 36.0 33.0 39.0 2.0 41.0 72 31.78045919898968 35.0 32.0 39.0 2.0 41.0 73 31.57825921555231 35.0 32.0 39.0 2.0 40.0 74 31.317055112160077 35.0 32.0 38.0 2.0 40.0 75 31.092506443899257 35.0 32.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5896763756542249 1101 2 0.61774270827366 1101 3 0.6330166517633202 1101 4 0.574052749541174 1101 5 0.6746133810556714 1101 6 0.6303392547020863 1101 7 0.6836919287865655 1101 8 0.6770717548353389 1101 9 0.8301697527974738 1101 10 0.8415049654399098 1101 11 0.8712417836092925 1101 12 0.9113890450298001 1101 13 0.8948813236009912 1101 14 1.0324133203871142 1101 15 1.0148018478263836 1101 16 0.9990021200189361 1101 17 1.0018431056040171 1101 18 0.9641208519420914 1101 19 1.0210857500581056 1101 20 1.0663705449672278 1101 21 1.0231319931209484 1101 22 1.0749087613318409 1101 23 1.0095271945116195 1101 24 0.9846791344769059 1101 25 1.074202330581862 1101 26 1.122420232600426 1101 27 1.0838276592333926 1101 28 1.0213345996588359 1101 29 1.0767579464172314 1101 30 1.0694448253816802 1101 31 1.101487973016006 1101 32 1.04986306175838 1101 33 1.0871278111513902 1101 34 1.070941696851932 1101 35 1.0984010383689338 1101 36 0.9363634251328037 1101 37 1.059230601075086 1101 38 1.0654927223407924 1101 39 1.0579054807470598 1101 40 1.0482518398563982 1101 41 1.1139908085314545 1101 42 1.1362692887770436 1101 43 1.2091950609639994 1101 44 1.136320651331154 1101 45 1.135842801720294 1101 46 1.1939192612789853 1101 47 1.1634215712097031 1101 48 1.2316535089000382 1101 49 1.2283465650731529 1101 50 1.083507641561873 1101 51 1.1415146631897457 1101 52 1.1028466285259526 1101 53 1.1938718945112612 1101 54 1.2617655738695461 1101 55 1.1700024112737921 1101 56 1.1771487072548439 1101 57 1.1817995372546264 1101 58 1.1962979307761827 1101 59 1.2425030825068148 1101 60 1.2346872926060186 1101 61 1.2332552907615124 1101 62 1.2924796247765542 1101 63 1.2899512807864681 1101 64 1.3578293795580123 1101 65 1.2122713998264274 1101 66 1.2611370005665279 1101 67 1.3355230783106578 1101 68 1.4555544360812291 1101 69 1.32986275919734 1101 70 1.3085700040629291 1101 71 1.3537475607536855 1101 72 1.3518006241831344 1101 73 1.2842789305603617 1101 74 1.3937727286306973 1101 75 1.4511859892959045 1102 1 0.7015931254436225 1102 2 0.6704727045862136 1102 3 0.687560949665702 1102 4 0.6724200497064672 1102 5 0.6658134941968044 1102 6 0.76265597762265 1102 7 0.8041983445634528 1102 8 0.7844057021084581 1102 9 0.9300903100244255 1102 10 0.9311040460340294 1102 11 0.9690473404102207 1102 12 0.9859867542587679 1102 13 0.9114027559744997 1102 14 1.207730257678051 1102 15 1.0724909101916893 1102 16 1.1355693105311815 1102 17 1.0908165466710216 1102 18 1.0749644082714696 1102 19 1.090425628754609 1102 20 1.0987403784531438 1102 21 1.0497732922622234 1102 22 1.1841617774381987 1102 23 1.117539414527947 1102 24 1.2060401520707202 1102 25 1.157509791897148 1102 26 1.0925595714741902 1102 27 1.1595775897447567 1102 28 1.205105797983009 1102 29 1.2098624828170372 1102 30 1.2333669365832804 1102 31 1.3043864938978302 1102 32 1.2964343290829206 1102 33 1.3936254737155664 1102 34 1.312154110316385 1102 35 1.3032479866236244 1102 36 1.2977125426902916 1102 37 1.3311864286094846 1102 38 1.353940218118204 1102 39 1.3916361995417645 1102 40 1.4088316763177886 1102 41 1.3872872075115765 1102 42 1.34008032673308 1102 43 1.365821434673002 1102 44 1.4496562952845125 1102 45 1.4429519705294922 1102 46 1.4282384081978208 1102 47 1.4788944918880134 1102 48 1.522380487803666 1102 49 1.55215880857007 1102 50 1.4925128759617294 1102 51 1.4197031433293503 1102 52 1.3816955354101808 1102 53 1.44226953755404 1102 54 1.4966113025436627 1102 55 1.5113541826435934 1102 56 1.4550589208536664 1102 57 1.43696855118489 1102 58 1.3999965888600485 1102 59 1.5338729541494658 1102 60 1.4306641555808497 1102 61 1.4921655997365875 1102 62 1.4591847757775653 1102 63 1.4722080339759671 1102 64 1.4579640263693179 1102 65 1.4881113215935642 1102 66 1.4775202013293622 1102 67 1.4621281769062122 1102 68 1.4671524631998523 1102 69 1.4736869688730962 1102 70 1.542875111297569 1102 71 1.492105715780685 1102 72 1.4858037939694313 1102 73 1.5003872031073158 1102 74 1.5266429895001998 1102 75 1.4972983648625693 1103 1 0.7120016098627673 1103 2 0.7510415843144251 1103 3 0.7574482656186952 1103 4 0.741579602744153 1103 5 0.824815616940846 1103 6 0.9355097956917291 1103 7 0.9687080911392911 1103 8 1.0307336911450804 1103 9 1.1054058207792892 1103 10 1.202332062270898 1103 11 1.2263356262092344 1103 12 1.1430540788046812 1103 13 1.0505776243697795 1103 14 1.3161122802917973 1103 15 1.266123318217062 1103 16 1.2955495712820309 1103 17 1.3582572881665769 1103 18 1.2834271880697514 1103 19 1.3400970675346215 1103 20 1.4160450693546238 1103 21 1.3464281404468252 1103 22 1.3880419452272008 1103 23 1.459284004833087 1103 24 1.4634984655901704 1103 25 1.4605384360938913 1103 26 1.4946703740884857 1103 27 1.5304821135859257 1103 28 1.4666999969147838 1103 29 1.4715767840179126 1103 30 1.4098501165970205 1103 31 1.4516529647574217 1103 32 1.426983640965716 1103 33 1.5110716429961144 1103 34 1.4832983182027348 1103 35 1.4963345051573782 1103 36 1.5351231102481506 1103 37 1.6315394694538554 1103 38 1.539519223080923 1103 39 1.5944742744917448 1103 40 1.5007474235739124 1103 41 1.4623852269668305 1103 42 1.5339926343679124 1103 43 1.5568214342841245 1103 44 1.6077840794921698 1103 45 1.5535831212650777 1103 46 1.5653602008427967 1103 47 1.6128193206704822 1103 48 1.5638491240757517 1103 49 1.5982714391319348 1103 50 1.5559703681192616 1103 51 1.5786020050440115 1103 52 1.600209962773782 1103 53 1.603093954228683 1103 54 1.647492447872402 1103 55 1.5992975510883625 1103 56 1.5769902443121708 1103 57 1.5458970316446994 1103 58 1.6261691736370736 1103 59 1.5916739435229488 1103 60 1.556745782706649 1103 61 1.5621420276845157 1103 62 1.5728029881036036 1103 63 1.5656939650944537 1103 64 1.5638560283150085 1103 65 1.6046039416755704 1103 66 1.6134450993314289 1103 67 1.6229489441470264 1103 68 1.6355436817885831 1103 69 1.6344479691565823 1103 70 1.650732303447711 1103 71 1.6033724662706597 1103 72 1.635106379686036 1103 73 1.6179629272888505 1103 74 1.6860988385749316 1103 75 1.664407974082092 1104 1 0.5492086510107406 1104 2 0.6026078974838391 1104 3 0.5394645031062737 1104 4 0.6136842223329637 1104 5 0.6046498039482131 1104 6 0.7164284316753964 1104 7 0.8078611295087299 1104 8 0.7597007660419806 1104 9 0.8953763089301603 1104 10 0.9172290476942635 1104 11 0.9688372000418397 1104 12 0.9154140824066275 1104 13 0.934159439523583 1104 14 1.0275155101949238 1104 15 1.0458293546099924 1104 16 1.0238763257556656 1104 17 1.0789091678156453 1104 18 1.061794695188702 1104 19 1.0931953098204517 1104 20 1.0803733262161046 1104 21 1.1053983517600656 1104 22 1.0953515662838385 1104 23 1.0434459041411444 1104 24 1.1457082759797572 1104 25 1.1694839604589191 1104 26 1.1611107942056051 1104 27 1.1579482485674362 1104 28 1.1129603727736423 1104 29 1.0832208032434423 1104 30 1.0567095381707787 1104 31 1.0774872921737852 1104 32 1.0569871465758212 1104 33 1.037622471660157 1104 34 1.1940330478153243 1104 35 1.138970263614901 1104 36 1.143485602677515 1104 37 1.1131255558333137 1104 38 1.1314344508870278 1104 39 1.2142249521786113 1104 40 1.1761043632714774 1104 41 1.2531101394866582 1104 42 1.2307609948782314 1104 43 1.2148983178457797 1104 44 1.26217162137187 1104 45 1.161730298741304 1104 46 1.1956954616559727 1104 47 1.1688197547038968 1104 48 1.1458996388962603 1104 49 1.223729160072331 1104 50 1.150644453965235 1104 51 1.1446023692767326 1104 52 1.1208957159501054 1104 53 1.1274522046217754 1104 54 1.11764642070586 1104 55 1.0807133180038875 1104 56 1.1485592906691195 1104 57 1.1055985465678333 1104 58 1.0869044080067454 1104 59 1.1200919119293076 1104 60 1.1958433670199753 1104 61 1.27339628213781 1104 62 1.2432191923406677 1104 63 1.1657784700843692 1104 64 1.1657086541277337 1104 65 1.0996978758163607 1104 66 1.1315251233474797 1104 67 1.132841115918346 1104 68 1.1948646387088004 1104 69 1.1328148924879073 1104 70 1.175940334034948 1104 71 1.220060768268386 1104 72 1.1884669918231268 1104 73 1.1757787274087406 1104 74 1.1754319367779935 1104 75 1.2168676669209653 1105 1 0.4732897253109485 1105 2 0.47150535179962816 1105 3 0.5306505731564961 1105 4 0.5316623560720402 1105 5 0.4840480192818859 1105 6 0.5425057376582316 1105 7 0.6221002471303478 1105 8 0.6524942040687733 1105 9 0.7441955050139555 1105 10 0.7767494082964177 1105 11 0.7468041708182298 1105 12 0.717237272095467 1105 13 0.7520570848766681 1105 14 0.814594466790993 1105 15 0.790711126243437 1105 16 0.8301234947652603 1105 17 0.8448826873971313 1105 18 0.8325332014854183 1105 19 0.8221445597953831 1105 20 0.898143336264539 1105 21 0.8936185442194713 1105 22 0.879845395313609 1105 23 0.9160899229584558 1105 24 0.9417907588682866 1105 25 0.946849582180576 1105 26 1.008586438889978 1105 27 0.9901751598645561 1105 28 0.941014640445438 1105 29 0.8786927094271206 1105 30 0.9398705287426665 1105 31 1.0030204051113003 1105 32 1.020307403617693 1105 33 1.0198291791143248 1105 34 1.1342982288654184 1105 35 1.0503751514540554 1105 36 1.0464567231253952 1105 37 1.0281088598829413 1105 38 1.0099989355626704 1105 39 1.0024473337802462 1105 40 0.9904392489375695 1105 41 0.9193467274574445 1105 42 1.0324365698758342 1105 43 1.0160195410697312 1105 44 1.0970480321008367 1105 45 1.1254676566528161 1105 46 1.024435734451913 1105 47 1.1619786021332672 1105 48 1.0975552317362016 1105 49 1.069863830448753 1105 50 1.0858669320553815 1105 51 1.0813418952149334 1105 52 1.009968748339375 1105 53 0.9590235648112113 1105 54 1.0296310709948244 1105 55 1.03092986850654 1105 56 1.0091652441272885 1105 57 0.9827308888607789 1105 58 0.9940685394157995 1105 59 1.0277503461095847 1105 60 1.0491676238186614 1105 61 1.0614817730740143 1105 62 1.100622098202507 1105 63 1.0559198592985055 1105 64 1.0546556307200348 1105 65 1.0147242699438763 1105 66 1.0146830657466595 1105 67 1.0186470336630222 1105 68 1.0435796427071722 1105 69 1.0354384658253082 1105 70 0.990750125009555 1105 71 0.9776933234623435 1105 72 1.028844800083867 1105 73 1.0421956896142248 1105 74 1.1139115536747788 1105 75 1.0791659658687855 1106 1 0.359816676307787 1106 2 0.39230901986121935 1106 3 0.40981899707686864 1106 4 0.3568873305038167 1106 5 0.3311879168712437 1106 6 0.3425752682765264 1106 7 0.4528893120403765 1106 8 0.4570566727129446 1106 9 0.6086664055516806 1106 10 0.5910313674629606 1106 11 0.538908025662316 1106 12 0.47837775394001625 1106 13 0.45914484176581993 1106 14 0.5570331869143317 1106 15 0.5371432163428125 1106 16 0.6046380885415132 1106 17 0.598546650486881 1106 18 0.5837920551319726 1106 19 0.591409949685918 1106 20 0.6527102343044788 1106 21 0.6535225077095177 1106 22 0.7047364635184223 1106 23 0.66411008527745 1106 24 0.7154802179528446 1106 25 0.7698986512501804 1106 26 0.7014577588496422 1106 27 0.7481897768974903 1106 28 0.6423666459125528 1106 29 0.6473980289229431 1106 30 0.6070616835742513 1106 31 0.6891597967568615 1106 32 0.6903090255324855 1106 33 0.7116819398115766 1106 34 0.7301378035487289 1106 35 0.7770191816172058 1106 36 0.7057990639818286 1106 37 0.7724113427684358 1106 38 0.6366045848030026 1106 39 0.812234552494445 1106 40 0.7240324401559448 1106 41 0.6726525457196502 1106 42 0.7875741645560197 1106 43 0.7718449363062092 1106 44 0.8076019357758852 1106 45 0.8084445969198342 1106 46 0.7985549242865133 1106 47 0.875928842196295 1106 48 0.8039579051596917 1106 49 0.8854406312617726 1106 50 0.8145275007994854 1106 51 0.7699576325016437 1106 52 0.7275345751730526 1106 53 0.7554819205683856 1106 54 0.7915485600544301 1106 55 0.7631406793366864 1106 56 0.7522563888249252 1106 57 0.7520729491305289 1106 58 0.7898132957066224 1106 59 0.890399746750326 1106 60 0.7594173209504902 1106 61 0.7391925074624552 1106 62 0.7878242220096752 1106 63 0.8733575174698345 1106 64 0.8340517602863073 1106 65 0.7765030259699301 1106 66 0.7468356339904929 1106 67 0.8446570320723126 1106 68 0.7910907137081722 1106 69 0.7801928197142018 1106 70 0.8463683138383686 1106 71 0.805566412086602 1106 72 0.8528395243009115 1106 73 0.7518064131154674 1106 74 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2214 26 -0.7153863505710163 2214 27 -0.7800006933203747 2214 28 -0.9038592442905156 2214 29 -0.7367576986040518 2214 30 -0.7961000016343931 2214 31 -0.7376467353707241 2214 32 -0.6701206800953301 2214 33 -0.6688195841006745 2214 34 -0.7218070596754416 2214 35 -0.6921100185483553 2214 36 -0.710498945525373 2214 37 -0.755176761936255 2214 38 -0.7176572521697366 2214 39 -0.7111710840995187 2214 40 -0.7481381627614354 2214 41 -0.8207306398213703 2214 42 -0.8245577824143453 2214 43 -0.7444815948179766 2214 44 -0.7335415126556768 2214 45 -0.6879378235294382 2214 46 -0.6559693015284367 2214 47 -0.6079501478825975 2214 48 -0.637527300976096 2214 49 -0.6505691267936413 2214 50 -0.6838654553394363 2214 51 -0.6557819101992521 2214 52 -0.607272910183589 2214 53 -0.6440573687742628 2214 54 -0.6718053764053238 2214 55 -0.7056729769238927 2214 56 -0.7478498460456962 2214 57 -0.7574132102343185 2214 58 -0.8249766803372225 2214 59 -0.7418671072174874 2214 60 -0.6393685913273544 2214 61 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-1.6117022679354207 2215 23 -1.63492125763716 2215 24 -1.7394616210637608 2215 25 -1.6692257550779246 2215 26 -1.6732098727475915 2215 27 -1.6743830701376723 2215 28 -1.5150831748211289 2215 29 -1.6807544514514632 2215 30 -1.6890059080081627 2215 31 -1.7582636451638365 2215 32 -1.7413292604500512 2215 33 -1.7211106962504417 2215 34 -1.7907345645338708 2215 35 -1.803700434528281 2215 36 -1.854781841395095 2215 37 -1.7566338478628012 2215 38 -1.863882486481316 2215 39 -1.8580641002614797 2215 40 -1.8546765013016966 2215 41 -1.8626333728414721 2215 42 -1.8136775580656206 2215 43 -1.9366327842106088 2215 44 -1.7604684271227242 2215 45 -1.8137476191064223 2215 46 -1.736573025914062 2215 47 -1.8410644132143261 2215 48 -1.989582453370872 2215 49 -1.9416488542296193 2215 50 -1.8584621934270587 2215 51 -1.8777795978330118 2215 52 -1.8842979772176136 2215 53 -1.9561261493700641 2215 54 -1.9235120010886035 2215 55 -1.8824672501275046 2215 56 -1.9803304738285306 2215 57 -1.9893750807910102 2215 58 -1.9484740112761898 2215 59 -1.9236000043171906 2215 60 -1.9293826623219168 2215 61 -2.0670111308833903 2215 62 -1.982567214671242 2215 63 -1.8671902049048548 2215 64 -1.9474984633540302 2215 65 -1.9339665715473338 2215 66 -1.8992557949366997 2215 67 -1.9119453232127412 2215 68 -1.8236349357388946 2215 69 -1.7627936849019754 2215 70 -1.7842367146290492 2215 71 -1.8135875657934477 2215 72 -1.9180069676308626 2215 73 -1.881288357315757 2215 74 -1.8223661268113283 2215 75 -1.7940397493766085 2216 1 -1.1966534554980157 2216 2 -1.1857996061645188 2216 3 -1.3052439883429479 2216 4 -1.116014174774989 2216 5 -1.3199313916015996 2216 6 -1.4586575776301274 2216 7 -1.4958525205972037 2216 8 -1.5630604377292485 2216 9 -2.1515965908947408 2216 10 -2.475650875380019 2216 11 -2.4900905404095326 2216 12 -2.0826289001036287 2216 13 -2.120626317357342 2216 14 -2.3643277115575643 2216 15 -2.242402072578834 2216 16 -2.291092584163131 2216 17 -2.5785483100255746 2216 18 -2.332395600307116 2216 19 -2.682394291696248 2216 20 -2.6081333642703584 2216 21 -2.605031098821435 2216 22 -2.682571668763181 2216 23 -2.7681637582955005 2216 24 -2.7941360057768705 2216 25 -2.760739997323469 2216 26 -2.82805942380368 2216 27 -2.758236584851602 2216 28 -2.607203530785057 2216 29 -2.685715022608008 2216 30 -2.7661009463814565 2216 31 -2.6737982787036145 2216 32 -2.675613498541516 2216 33 -2.646189910186031 2216 34 -2.7705544726922824 2216 35 -2.56457309222386 2216 36 -2.736376039837701 2216 37 -2.672540563362233 2216 38 -2.780227347972996 2216 39 -2.748510204008589 2216 40 -2.7079977792531764 2216 41 -2.6117597350784436 2216 42 -2.583210986435425 2216 43 -2.5663957999757088 2216 44 -2.672576660198594 2216 45 -2.658645380029739 2216 46 -2.495314267059932 2216 47 -2.7107593532617535 2216 48 -2.776537117488502 2216 49 -2.6269331201213646 2216 50 -2.638471481054271 2216 51 -2.643535685497067 2216 52 -2.614496948091567 2216 53 -2.63862572270013 2216 54 -2.8099069957608336 2216 55 -2.664600866152302 2216 56 -2.846384159554102 2216 57 -2.7846592135114108 2216 58 -2.790780822462608 2216 59 -2.9123988521948263 2216 60 -2.8585361215518788 2216 61 -2.7873101625313446 2216 62 -2.8040374668014145 2216 63 -2.7785685731042236 2216 64 -2.6962052438067126 2216 65 -2.642162721520851 2216 66 -2.768446494274656 2216 67 -2.796352812302665 2216 68 -2.799851423738101 2216 69 -2.8691642030495785 2216 70 -2.7140527473172753 2216 71 -2.668076854613677 2216 72 -2.8367272192547404 2216 73 -2.815157831111595 2216 74 -2.8031823959553996 2216 75 -2.8152440638580956 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80579.0 3 31459.0 4 18237.0 5 11620.0 6 8544.0 7 7719.0 8 7486.0 9 7766.0 10 8565.0 11 9147.0 12 9791.0 13 10270.0 14 10871.0 15 11445.0 16 12367.0 17 13692.0 18 14543.0 19 15668.0 20 18515.0 21 21396.0 22 27566.0 23 33734.0 24 35529.0 25 36704.0 26 36722.0 27 38062.0 28 42606.0 29 50928.0 30 58395.0 31 66025.0 32 79671.0 33 99094.0 34 128438.0 35 174554.0 36 243612.0 37 374985.0 38 638512.0 39 1075357.0 40 293946.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.186829579062405 19.03337666844496 14.443981167354677 25.33581258513796 2 14.952047254672005 20.512795293168796 38.60361005111698 25.931547401042216 3 18.046335163896668 25.038198560048862 29.424511260293684 27.490955015760786 4 12.043792310198036 16.705594489136793 36.96963163387236 34.28098156679281 5 13.090954023036147 38.14517165202903 34.51909248030239 14.24478184463243 6 31.826172304935245 37.59678459422853 16.9673513043163 13.609691796519924 7 27.712465505904767 32.39820035309528 21.41947408477393 18.469860056226022 8 26.188723964326176 35.33119533391852 19.51698021776738 18.963100483987912 9 25.976020645019638 15.818311606298833 19.44537870390724 38.76028904477429 10 13.842370767840443 30.096446990914462 33.715933751165885 22.345248490079218 11 34.573626877639775 23.670283945050773 23.12613918903459 18.629949988274863 12 23.12194148901721 25.687865434583813 30.332294508216044 20.857898568182936 13 27.854343251618594 22.486316123196854 26.120637762423236 23.53870286276131 14 21.551634409994175 21.525380283513496 28.040623988607678 28.88236131788465 15 23.165445614877918 29.621498104229094 24.417705319961815 22.795350960931174 16 22.666516171655438 28.723483020641975 25.979442933741463 22.63055787396112 17 21.4924352415833 28.30665842143505 27.39152054375042 22.809385793231232 18 21.490418053305426 27.494985581200194 28.93111926693843 22.083477098555946 19 22.308440718931124 27.09844188106903 29.36543905389033 21.227678346109517 20 22.53246077607694 26.542931877276704 29.265661486505024 21.65894586014133 21 22.81017660548737 26.36491115622841 29.191769024055553 21.633143214228674 22 22.28983006669933 26.39459944811085 29.112786812432496 22.202783672757327 23 22.198309164176933 26.382484632588877 28.9728598150967 22.44634638813749 24 21.782385297353265 26.534886429819455 29.32569727560991 22.357030997217368 25 22.246555500884046 26.446785397306417 29.163663112961142 22.142995988848394 26 21.954783822496566 26.584353766327766 29.34345132741072 22.11741108376495 27 22.04413989844536 26.8484795926424 28.957699371852303 22.149681137059936 28 21.991622825741675 26.64520292991398 29.1120138330297 22.251160411314647 29 21.99215409276875 26.73615489439666 29.214728307433624 22.056962705400966 30 22.21666777554248 26.735470469256395 29.032111921726656 22.015749833474466 31 22.203455945712918 26.33159674093194 29.209696444476542 22.2552508688786 32 21.990275996568 26.773207096426034 28.99378435074742 22.24273255625855 33 22.0033999713218 26.541310842171033 29.0239162720826 22.431372914424568 34 22.174592184699403 26.664216431672738 29.177277521466515 21.983913862161337 35 22.3125313964927 26.65836242105424 28.92217086058676 22.1069353218663 36 22.22795355395481 26.67694510413712 28.904805484409973 22.1902958574981 37 22.3349175205516 26.401808991174775 28.97305277095593 22.290220717317695 38 22.161571543847316 26.530542527966972 29.07576613176633 22.23211979641938 39 22.33994259492672 26.806668098773994 28.779482215556307 22.073907090742978 40 22.42371035402371 26.45606693694328 29.061939655707903 22.058283053325106 41 22.13676759974832 26.66854571934459 28.788795255469633 22.405891425437456 42 22.290372564309678 26.59272128810617 28.761908804265595 22.354997343318555 43 22.118251055082748 26.47570527357611 28.834623382176254 22.571420289164884 44 22.16624280939467 26.824083099146367 28.61963153663747 22.39004255482149 45 22.354241903650767 26.7659479976459 28.418344978728754 22.461465119974573 46 22.425846336357544 26.60885493591288 28.26072835223886 22.70457037549071 47 22.3533156828187 26.826478877613606 28.180702966684006 22.639502472883684 48 22.252689660884037 26.9813684207802 28.133891668197947 22.632050250137816 49 22.4332670126934 26.886566861349838 27.98841424658442 22.691751879372347 50 22.587254380316885 26.932582559889696 27.931960423425455 22.548202636367964 51 22.44624163765997 27.171491052134417 27.641981858412805 22.740285451792808 52 22.483885844543234 27.110859902991567 27.71546746708199 22.689786785383205 53 22.493366531085886 27.12091792251632 27.793024900643537 22.592690645754256 54 22.34561677464301 27.21689658545624 27.95680670207276 22.480679937827986 55 22.410064718620994 27.02662345190823 27.812392601456686 22.750919228014094 56 22.35503055672956 27.269936800509964 27.757643582206832 22.61738906055365 57 22.47628236217269 27.006895959163586 27.67229416547921 22.844527513184516 58 22.214047274546346 26.915752117758586 27.99566471856772 22.87453588912735 59 22.334002357761136 27.14818308752832 27.862991043872213 22.654823510838327 60 22.245355657371423 27.259775738239817 27.895170256331685 22.599698348057068 61 22.56888587997325 26.946469941553886 27.952817649360572 22.531826529112294 62 22.23258501219591 27.139448035181367 27.91083306736081 22.717133885261912 63 22.19606472944206 27.00040763243201 28.040426784685963 22.763100853439965 64 22.247299134579073 26.893841061436678 28.0725393401971 22.786320463787142 65 22.16983928007995 26.949815824232825 28.009418576088756 22.87092631959847 66 22.284006842640242 27.100722390855154 27.79127617725327 22.82399458925134 67 22.27310364392024 27.020338562771247 27.730558886632128 22.97599890667639 68 22.323913166706745 27.03431571116896 27.925747175645387 22.71602394647891 69 22.399612201872426 27.064887861017954 27.580511332432334 22.954988604677283 70 22.188496657726674 26.84342693148094 27.924586221230385 23.043490189562004 71 22.374388688511694 26.87125500540397 27.74117040527726 23.013185900807077 72 22.17164892057133 27.10106616342421 27.621892082239867 23.105392833764594 73 22.24679180590581 26.937623969423292 27.778726743439712 23.036857481231184 74 22.241235652675616 27.046054060322245 27.52304929062878 23.189660996373355 75 22.33017975083791 27.119092790996852 27.51349548183768 23.037231976327558 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1293.0 1 978.0 2 873.0 3 1120.0 4 1157.0 5 895.0 6 799.5 7 1699.0 8 2432.0 9 4175.0 10 6788.5 11 7372.0 12 7085.0 13 6556.5 14 6177.5 15 6774.0 16 7221.0 17 7841.0 18 9258.5 19 11088.5 20 12121.0 21 13661.5 22 17657.5 23 22366.0 24 24619.0 25 28149.0 26 36338.5 27 45298.0 28 49598.0 29 55889.5 30 66982.0 31 78422.5 32 85062.0 33 91188.0 34 104487.5 35 118439.0 36 125217.0 37 130107.0 38 138284.0 39 146319.0 40 151067.0 41 156095.0 42 164678.5 43 171246.5 44 174259.0 45 177224.5 46 181710.0 47 185686.5 48 188143.0 49 188917.0 50 187328.0 51 181230.5 52 177496.0 53 172244.5 54 160695.0 55 146727.5 56 139058.0 57 131580.0 58 115070.0 59 96005.0 60 85972.0 61 77798.0 62 61284.5 63 45533.5 64 38122.0 65 31494.5 66 21099.0 67 14954.5 68 12578.0 69 10329.0 70 6601.0 71 4169.5 72 3217.0 73 2815.0 74 2063.0 75 1297.0 76 881.0 77 734.5 78 413.5 79 204.5 80 170.0 81 135.5 82 64.0 83 23.5 84 20.0 85 14.5 86 7.0 87 4.0 88 3.0 89 3.5 90 3.0 91 1.0 92 0.0 93 1.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07090877094914237 2 0.016277962382120637 3 0.0025879113485088455 4 0.13635704895293108 5 0.09709843379605189 6 0.07013239754458972 7 0.029502189373000837 8 0.026060267279484076 9 0.04867861246545138 10 0.030252683664068403 11 0.01620032504166537 12 0.10325766280550293 13 0.08726437067171827 14 0.04839394221711541 15 0.02028922497230935 16 0.03395339689243605 17 0.022695982526422572 18 0.019694005362152316 19 0.01425939153028374 20 0.013819446601037233 21 0.028544662174052566 22 0.017157852240613645 23 0.015191039615746924 24 0.022514828732026957 25 0.0165885117439417 26 0.033616968417129904 27 0.030407958344978937 28 0.038326967071416 29 0.05121476558699005 30 0.03491092409138433 31 0.020884444582466383 32 0.0125513700402679 33 0.014440545324679357 34 0.014104116849373207 35 0.00602983344202561 36 0.00952351376251255 37 0.007996646066892332 38 0.0025361531215386686 39 0.00905768971978096 40 0.010972744117677504 41 0.012189062451476662 42 0.007168514435369502 43 0.011438568160409097 44 0.010299887167065204 45 0.005641646739749283 46 0.0021479664192623417 47 0.005434613831868575 48 0.0064697783712721136 49 0.001656263263045661 50 4.1406581576141526E-4 51 0.0018115379439561919 52 0.003260768299121145 53 7.763734045526535E-5 54 5.175822697017691E-5 55 2.0703290788070763E-4 56 0.00835895365568357 57 0.0030537353912404377 58 2.8467024833597304E-4 59 0.007375547343250209 60 0.001656263263045661 61 0.0012421974472842457 62 0.02430048756249806 63 0.008928294152355517 64 0.011671480181774893 65 0.017882467418196123 66 0.01808950032607683 67 0.017364885148494352 68 0.017364885148494352 69 0.006573294825212467 70 0.01506164404832148 71 0.007660217591586183 72 0.0165885117439417 73 0.0068320859600633525 74 0.019901038270033023 75 0.0012680765607693343 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3864120.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.125023382637515 #Duplication Level Percentage of deduplicated Percentage of total 1 73.26572364519288 41.12040452732309 2 13.446410580899856 15.09360216531098 3 5.47709935830074 9.22206988660974 4 2.7414253897690393 6.15450256410174 5 1.5251533351215825 4.279963329790321 6 0.9183427836623594 3.0925206123795808 7 0.6161545789102768 2.420718310406092 8 0.41652995610466076 1.8702202820754452 9 0.2989288426148368 1.5099649453352237 >10 1.2283097589524272 11.377728313756261 >50 0.04755202985078942 1.8255776584526582 >100 0.017860929760876304 1.6392376381293354 >500 3.237887501544691E-4 0.10332450129926221 >1k 1.8502210956516802E-4 0.2901652650302244 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4321 0.11182364936906722 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 2.5879113485088454E-5 0.0 11 0.0 0.0 0.0 2.5879113485088454E-5 0.0 12 0.0 0.0 0.0 2.5879113485088454E-5 0.0 13 0.0 0.0 0.0 2.5879113485088454E-5 0.0 14 0.0 0.0 0.0 5.175822697017691E-5 0.0 15 0.0 0.0 0.0 5.175822697017691E-5 0.0 16 0.0 0.0 0.0 5.175822697017691E-5 0.0 17 0.0 0.0 0.0 7.763734045526537E-5 0.0 18 0.0 0.0 0.0 7.763734045526537E-5 0.0 19 0.0 0.0 0.0 7.763734045526537E-5 0.0 20 0.0 0.0 0.0 7.763734045526537E-5 0.0 21 0.0 0.0 0.0 1.2939556742544228E-4 0.0 22 0.0 0.0 0.0 1.5527468091053074E-4 0.0 23 0.0 0.0 0.0 3.1054936182106147E-4 0.0 24 0.0 0.0 0.0 4.1406581576141526E-4 0.0 25 0.0 0.0 0.0 4.3994492924650375E-4 0.0 26 0.0 0.0 0.0 6.469778371272114E-4 0.0 27 0.0 0.0 0.0 9.057689719780959E-4 0.0 28 0.0 0.0 0.0 0.0015786259225903957 0.0 29 0.0 0.0 0.0 0.002665548688964111 0.0 30 0.0 0.0 0.0 0.004037141703673799 0.0 31 0.0 0.0 0.0 0.007996646066892333 0.0 32 0.0 0.0 0.0 0.0138970839414925 0.0 33 0.0 0.0 0.0 0.020030433837458464 0.0 34 2.5879113485088454E-5 0.0 0.0 0.028932848876328892 0.0 35 2.5879113485088454E-5 0.0 0.0 0.03858575820626688 0.0 36 2.5879113485088454E-5 0.0 0.0 0.050697183317288286 0.0 37 2.5879113485088454E-5 0.0 0.0 0.06715629949380454 0.0 38 2.5879113485088454E-5 0.0 0.0 0.08804074407627092 0.0 39 2.5879113485088454E-5 0.0 0.0 0.11104727596451457 0.0 40 2.5879113485088454E-5 0.0 0.0 0.13389853317184766 0.0 41 2.5879113485088454E-5 0.0 0.0 0.15602517520159828 0.0 42 2.5879113485088454E-5 0.0 0.0 0.1777377514155875 0.0 43 2.5879113485088454E-5 0.0 0.0 0.20227115099945137 0.0 44 2.5879113485088454E-5 0.0 0.0 0.2299876815419811 0.0 45 2.5879113485088454E-5 0.0 0.0 0.26339761705123027 0.0 46 2.5879113485088454E-5 0.0 0.0 0.2985673322774655 0.0 47 2.5879113485088454E-5 0.0 0.0 0.3355227063341718 0.0 48 2.5879113485088454E-5 0.0 0.0 0.37268511329875886 0.0 49 2.5879113485088454E-5 0.0 0.0 0.41142614618593626 0.0 50 2.5879113485088454E-5 0.0 0.0 0.45050360754841984 0.0 51 2.5879113485088454E-5 0.0 0.0 0.4931006283448754 0.0 52 2.5879113485088454E-5 0.0 0.0 0.5334979244950985 0.0 53 2.5879113485088454E-5 0.0 0.0 0.5739210997588067 0.0 54 2.5879113485088454E-5 0.0 0.0 0.6182520211587632 2.5879113485088454E-5 55 2.5879113485088454E-5 0.0 0.0 0.6672670620995207 2.5879113485088454E-5 56 2.5879113485088454E-5 0.0 0.0 0.7215096839642661 2.5879113485088454E-5 57 2.5879113485088454E-5 0.0 0.0 0.7803587880293573 2.5879113485088454E-5 58 2.5879113485088454E-5 0.0 0.0 0.8428309679823608 2.5879113485088454E-5 59 2.5879113485088454E-5 0.0 0.0 0.9057172137511258 2.5879113485088454E-5 60 2.5879113485088454E-5 0.0 0.0 0.9726923594505347 2.5879113485088454E-5 61 2.5879113485088454E-5 2.5879113485088454E-5 0.0 1.0434717348322515 2.5879113485088454E-5 62 2.5879113485088454E-5 2.5879113485088454E-5 0.0 1.1133970994689606 2.5879113485088454E-5 63 2.5879113485088454E-5 2.5879113485088454E-5 0.0 1.1830636729708186 2.5879113485088454E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5035 0.0 41.428883 1 GTATCAA 10355 0.0 34.97695 1 ATCAACG 14460 0.0 25.061953 3 TATCAAC 14430 0.0 24.967356 2 TCAACGC 14590 0.0 24.814943 4 CAACGCA 14880 0.0 24.224815 5 AACGCAG 15470 0.0 23.343443 6 CGCGTAA 85 0.0041021416 20.31098 10 GTGGTAT 2445 0.0 20.09069 1 GTACATG 12320 0.0 19.851534 1 ACGCAGA 18170 0.0 19.779427 7 TACATGG 12030 0.0 19.63027 2 CGCAGAG 18725 0.0 19.212357 8 GAGTACT 13025 0.0 18.980824 12 TGGTATC 2385 0.0 18.8463 2 AGAGTAC 18330 0.0 18.780731 11 ACATGGG 12385 0.0 18.73477 3 AGTACTT 13780 0.0 17.984695 13 CAGAGTA 19435 0.0 17.961658 10 CGTGCCG 290 2.1827873E-11 17.859655 10 >>END_MODULE