FastQCFastQC Report
Mon 6 Feb 2017
SRR2077524_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077524_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3539212
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37150.037.353281
GTATCAA74350.033.056781
TCAACGC90000.026.6376694
ATCAACG90000.026.599343
TATCAAC92750.025.9587122
CAACGCA92500.025.953925
AACGCAG96450.024.8022736
ACGCAGA109350.021.7518027
CGCAGAG110150.021.4397758
AGAGTAC109700.020.08320211
GAGTACT77700.019.39177712
CAGAGTA117500.018.98476210
GCAGAGT120450.018.8060389
AGTACTT84350.017.86473513
GTACTTT84100.017.75433514
GTACATG77500.017.593691
GTGGTAT20350.017.4716661
TGGTATC20000.017.0744552
TACATGG76500.017.044022
GTATAGG9050.015.6385621
Produced by FastQC (version 0.11.5)