FastQCFastQC Report
Mon 6 Feb 2017
SRR2077524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3539212
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42950.040.681091
GTATCAA86350.034.2985571
TATCAAC115900.025.2984392
ATCAACG115650.025.2969153
TCAACGC116250.025.0484264
CAACGCA118600.024.404345
AACGCAG122600.023.712756
ACGCAGA141200.020.441827
CGCAGAG143100.020.1230058
GTGGTAT20050.020.0174161
AGAGTAC142200.019.57132711
GAGTACT110850.019.25030512
TGGTATC20300.019.07672
CAGAGTA151000.018.6365510
GCAGAGT155350.018.2917399
CGAATCG2307.3141564E-917.9932737
AGTACTT118950.017.90331813
GTACTTT121400.017.47822614
GTACATG76100.017.4586621
TACATGG76650.017.2318732
Produced by FastQC (version 0.11.5)