FastQCFastQC Report
Mon 6 Feb 2017
SRR2077523_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077523_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3622552
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42550.037.3254781
GTATCAA83900.032.3038861
TCAACGC103900.025.7672844
TATCAAC104650.025.7134192
ATCAACG104450.025.5978623
CAACGCA106200.025.2102835
AACGCAG109700.024.3867116
ACGCAGA127300.020.9090887
CGCAGAG129900.020.3863898
GTACATG119050.019.8949011
AGAGTAC125450.019.70855111
GAGTACT70350.019.26374212
TACATGG118400.019.2307722
CCGATCG1451.2716957E-519.024639
GTGGTAT22950.018.5041661
CAGAGTA136950.018.48111710
AGTACTT74500.018.37758813
GCAGAGT142150.018.193189
GTGTACG4600.018.01361
GTACTTT75200.017.74791714
Produced by FastQC (version 0.11.5)