FastQCFastQC Report
Mon 6 Feb 2017
SRR2077523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3622552
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44390.12253792354119417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49800.043.15371
GTATCAA104150.035.2875141
TATCAAC142150.025.617492
ATCAACG145500.024.9842873
TCAACGC146200.024.8176924
CAACGCA148600.024.518165
AACGCAG154300.023.5186826
GTGGTAT20450.022.506821
GGACCGT4250.020.310816
ACGCAGA178550.020.246557
CGCAGAG182400.019.781618
TGGTATC22700.019.347862
AGAGTAC180850.019.28346311
GAGTACT132250.019.1899512
AGTACTT134350.018.959513
GTACATG106900.018.646641
CAGAGTA189400.018.50411410
GCAGAGT194800.018.185649
GTACTTT139600.018.13830814
TACATGG107850.018.0527482
Produced by FastQC (version 0.11.5)