FastQCFastQC Report
Mon 6 Feb 2017
SRR2077522_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077522_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4363081
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39050.035.3529621
GTATCAA76600.033.7473681
TCAACGC95900.025.9699424
ATCAACG96750.025.7420773
CAACGCA97150.025.6724785
TATCAAC97450.025.6621822
AACGCAG101650.024.3823036
ACGCAGA116550.020.9425247
CGCAGAG119100.020.3223198
GTACATG109550.019.7534071
AGAGTAC113900.019.3744411
TACATGG111250.018.820282
GAGTACT65800.018.18387412
CAGAGTA126800.017.75647710
ACATGGG114300.017.745513
CGTGCCG5550.017.39514210
GTACTTT69200.017.392714
GCAGAGT132700.017.3563529
AGTACTT72550.016.82726313
GCGATAC4750.015.97263914
Produced by FastQC (version 0.11.5)