FastQCFastQC Report
Mon 6 Feb 2017
SRR2077522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4363081
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46300.039.0700041
GTATCAA95200.034.333461
ATCAACG124900.026.0200713
TCAACGC125800.025.7242814
TATCAAC126550.025.622052
CAACGCA126800.025.3767665
AACGCAG130850.024.4593876
ACGCAGA150750.021.1148627
CGCAGAG154850.020.378618
GAGTACT104250.019.87214312
AGAGTAC150450.019.66790611
GCGATAC5150.018.75735314
GTACTTT112400.018.44713414
AGTACTT113600.018.44210213
CAGAGTA163750.018.40782210
TATGCCG5500.018.18326643
GCACCGT9550.018.0761626
GCAGAGT169500.017.9656739
GTACATG100850.017.9369471
TGCGATA5300.017.58279613
Produced by FastQC (version 0.11.5)