FastQCFastQC Report
Mon 6 Feb 2017
SRR2077521_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077521_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3033944
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34300.039.772261
GTATCAA66800.033.295591
TATCAAC87800.024.9142932
TCAACGC87800.024.876644
ATCAACG88900.024.568833
CAACGCA90650.024.1702585
GTGGTAT17200.023.4927981
AACGCAG94150.023.251766
TGGTATC17050.022.8669322
ACTCGAC3800.020.88635446
GTACATG104550.020.3485761
ACGCAGA107400.020.0320647
TGCGATA2603.6379788E-1219.89459213
GTATAGG6250.019.8929791
CGCAGAG110400.019.3643938
TACATGG108950.018.8743882
AGAGTAC106900.018.61233711
GCGCACT4650.018.55354942
ACATGGG108900.018.313973
TCGACAG4550.018.20226548
Produced by FastQC (version 0.11.5)