FastQCFastQC Report
Mon 6 Feb 2017
SRR2077521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3033944
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34090.1123619948159887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42500.041.845841
GTATCAA85200.034.2346651
GTGGTAT18650.026.3443571
TATCAAC116300.025.037322
ATCAACG121000.023.9814343
TCAACGC121350.023.9114744
TGGTATC19700.023.6986182
CAACGCA123250.023.3643155
AACGCAG127150.022.6446866
GCACCGT7000.019.7277936
TATATCG1056.0344697E-419.7102366
ACGCAGA147700.019.3086537
CGTGCCG4350.019.04972610
CGAATCG3850.018.8115237
GTACATG99300.018.7809521
CGCAGAG151350.018.6836228
AGAGTAC147100.018.37874611
GAGTACT101750.018.01884312
TACATGG101550.018.0148742
TACCGAT5050.017.7760147
Produced by FastQC (version 0.11.5)