##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077521_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3033944
Sequences flagged as poor quality	0
Sequence length	75
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.94445777509407	31.0	31.0	34.0	27.0	34.0
2	31.004181026413143	33.0	31.0	34.0	27.0	34.0
3	31.079818546420107	33.0	31.0	34.0	27.0	34.0
4	34.449720561750645	37.0	35.0	37.0	31.0	37.0
5	34.17974425368431	37.0	35.0	37.0	30.0	37.0
6	34.168101652502486	37.0	35.0	37.0	30.0	37.0
7	34.001296002826685	37.0	35.0	37.0	30.0	37.0
8	33.95462770571903	37.0	35.0	37.0	29.0	37.0
9	35.41045615871618	39.0	35.0	39.0	29.0	39.0
10	35.30339913986547	39.0	35.0	39.0	28.0	39.0
11	35.29983051763645	39.0	35.0	39.0	28.0	39.0
12	35.19721128669481	39.0	35.0	39.0	27.0	39.0
13	35.13126840838196	39.0	35.0	39.0	27.0	39.0
14	36.37019140761991	40.0	37.0	41.0	27.0	41.0
15	36.41481681929528	40.0	37.0	41.0	28.0	41.0
16	36.394492779036135	40.0	37.0	41.0	28.0	41.0
17	36.40056144740971	40.0	37.0	41.0	28.0	41.0
18	36.404950453930596	40.0	37.0	41.0	28.0	41.0
19	36.45065762584939	40.0	37.0	41.0	27.0	41.0
20	36.418091434779285	40.0	37.0	41.0	27.0	41.0
21	36.3954614851164	40.0	37.0	41.0	27.0	41.0
22	36.38164152008079	40.0	37.0	41.0	27.0	41.0
23	36.35155098446115	40.0	37.0	41.0	27.0	41.0
24	36.335952146776606	40.0	37.0	41.0	27.0	41.0
25	36.3076071938045	40.0	36.0	41.0	27.0	41.0
26	36.23820215534631	40.0	36.0	41.0	27.0	41.0
27	36.18682777269455	40.0	36.0	41.0	27.0	41.0
28	36.16400335668688	40.0	36.0	41.0	27.0	41.0
29	36.10891301882962	40.0	36.0	41.0	27.0	41.0
30	36.019297653483385	40.0	36.0	41.0	27.0	41.0
31	35.924516075445034	40.0	36.0	41.0	27.0	41.0
32	35.84513062864707	40.0	35.0	41.0	27.0	41.0
33	35.777915149389706	40.0	35.0	41.0	27.0	41.0
34	35.71823243935946	40.0	35.0	41.0	27.0	41.0
35	35.633045962615	40.0	35.0	41.0	26.0	41.0
36	35.57698362263773	40.0	35.0	41.0	26.0	41.0
37	35.508383147480636	40.0	35.0	41.0	25.0	41.0
38	35.45085077377829	40.0	35.0	41.0	25.0	41.0
39	35.371991704527176	40.0	35.0	41.0	25.0	41.0
40	35.298857526704516	40.0	35.0	41.0	25.0	41.0
41	35.244162054408385	40.0	35.0	41.0	25.0	41.0
42	35.17807909440649	40.0	35.0	41.0	24.0	41.0
43	35.07462069174645	40.0	35.0	41.0	24.0	41.0
44	34.93658386575362	39.0	35.0	41.0	24.0	41.0
45	34.833318611022484	39.0	35.0	41.0	23.0	41.0
46	34.681375134148816	39.0	35.0	41.0	22.0	41.0
47	34.5185850496911	39.0	34.0	41.0	21.0	41.0
48	34.3995881268738	39.0	34.0	41.0	20.0	41.0
49	34.29764853932703	39.0	34.0	41.0	18.0	41.0
50	34.28924067154832	39.0	34.0	41.0	18.0	41.0
51	34.20369756330374	39.0	34.0	41.0	18.0	41.0
52	34.13086662113737	39.0	34.0	41.0	17.0	41.0
53	34.03295973821534	39.0	34.0	41.0	15.0	41.0
54	33.992746405339055	39.0	34.0	41.0	15.0	41.0
55	33.90714363877514	39.0	34.0	41.0	14.0	41.0
56	33.778355170695306	39.0	34.0	41.0	13.0	41.0
57	33.69645121993023	38.0	34.0	41.0	13.0	41.0
58	33.58776167259514	38.0	33.0	41.0	11.0	41.0
59	33.4874104466002	38.0	33.0	41.0	8.0	41.0
60	33.453202498134445	38.0	33.0	40.0	8.0	41.0
61	33.33298867744428	37.0	33.0	40.0	7.0	41.0
62	33.18286197767658	37.0	33.0	40.0	6.0	41.0
63	33.04285379031386	37.0	33.0	40.0	2.0	41.0
64	32.885635661040546	37.0	33.0	40.0	2.0	41.0
65	32.75359202411119	37.0	33.0	40.0	2.0	41.0
66	32.550677599850225	36.0	33.0	40.0	2.0	41.0
67	32.35824590038577	36.0	33.0	39.0	2.0	41.0
68	32.14881817198999	36.0	33.0	39.0	2.0	41.0
69	31.983227772167186	36.0	32.0	39.0	2.0	41.0
70	31.79349915489541	35.0	32.0	39.0	2.0	41.0
71	31.61716564313646	35.0	32.0	39.0	2.0	41.0
72	31.42228201970768	35.0	32.0	38.0	2.0	40.0
73	31.22830678483189	35.0	32.0	37.0	2.0	40.0
74	30.97144508929631	35.0	32.0	37.0	2.0	40.0
75	30.75949753851752	35.0	32.0	37.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3569540232633166
1101	2	0.43832215319631374
1101	3	0.5046501151162737
1101	4	0.41281092607626135
1101	5	0.48835885707836724
1101	6	0.5301724237151006
1101	7	0.566840545633525
1101	8	0.6100362759341138
1101	9	0.6606923552749677
1101	10	0.6877722832620989
1101	11	0.7185776858272988
1101	12	0.7792553446383863
1101	13	0.74158951048706
1101	14	0.7812171628658362
1101	15	0.7257168119137702
1101	16	0.7420025290460899
1101	17	0.7822707183382676
1101	18	0.7232874138570224
1101	19	0.7573466381261866
1101	20	0.7353099268146792
1101	21	0.7453843923783481
1101	22	0.7905864564983887
1101	23	0.8158583109439874
1101	24	0.8363284689678352
1101	25	0.8771165499219293
1101	26	0.9466947421083205
1101	27	0.9111409638790633
1101	28	0.9977199263771723
1101	29	0.953594712174727
1101	30	0.8771970368234818
1101	31	0.994408783710675
1101	32	0.9152450109773369
1101	33	0.9579712249086754
1101	34	0.9617582728639249
1101	35	0.9619687390721197
1101	36	0.8987583553047216
1101	37	1.0280506365172997
1101	38	1.076333100244618
1101	39	1.0855460730301871
1101	40	0.9988617157439066
1101	41	0.9839677091085548
1101	42	1.031677488569109
1101	43	1.0798029339036361
1101	44	1.2273029080491114
1101	45	1.226701725974884
1101	46	1.1214084465716638
1101	47	1.1032782768349705
1101	48	1.2202311899808933
1101	49	1.2182154395230214
1101	50	1.1364358977223716
1101	51	1.1315981452175663
1101	52	1.0969479998969973
1101	53	1.1286806776080809
1101	54	1.0749859836943614
1101	55	1.0747021009844744
1101	56	1.0674169675892884
1101	57	1.1130427483187333
1101	58	1.0819979585534938
1101	59	1.0941298201111778
1101	60	1.0992750000851785
1101	61	1.1266508991249822
1101	62	1.2220945172009792
1101	63	1.1709143589746063
1101	64	1.1281995361405777
1101	65	1.0645434633396391
1101	66	1.130249749036878
1101	67	1.1978056918786706
1101	68	1.2453846944609168
1101	69	1.209284549296207
1101	70	1.2276228976191277
1101	71	1.224975777515379
1101	72	1.2007436637084403
1101	73	1.1874090323205806
1101	74	1.2486059821568674
1101	75	1.1883949433120407
1102	1	0.715351715307424
1102	2	0.7676688746956977
1102	3	0.7943849221882111
1102	4	0.7392214558729862
1102	5	0.8257653512917216
1102	6	0.8987643507211232
1102	7	0.9997940888009538
1102	8	1.0119138634136746
1102	9	1.0461214409421444
1102	10	1.174241858235746
1102	11	1.193653083260699
1102	12	1.0816375139316747
1102	13	1.0837767149710729
1102	14	1.3122136229485974
1102	15	1.267971613915627
1102	16	1.2285282340077615
1102	17	1.2692348567891614
1102	18	1.2589900242637597
1102	19	1.3042161232498444
1102	20	1.2584437779860949
1102	21	1.2166559198564713
1102	22	1.3443766798831405
1102	23	1.285638481967709
1102	24	1.3589133594051432
1102	25	1.3083255478583027
1102	26	1.353909246559283
1102	27	1.3263291295563064
1102	28	1.3091760411801658
1102	29	1.3636189189540318
1102	30	1.402967894784787
1102	31	1.4225937457255284
1102	32	1.4189274322307455
1102	33	1.5029295075683748
1102	34	1.4502006935250549
1102	35	1.5854842741620772
1102	36	1.5043164947883199
1102	37	1.5177857796340675
1102	38	1.548361358674434
1102	39	1.5641145514580472
1102	40	1.5480540057413492
1102	41	1.5403133433978908
1102	42	1.5826460380284502
1102	43	1.6293909691954838
1102	44	1.6622823289787618
1102	45	1.651166671720972
1102	46	1.6144456501998192
1102	47	1.7795919679629364
1102	48	1.7169179979845097
1102	49	1.7279176838509258
1102	50	1.7460037346196486
1102	51	1.6856962696778623
1102	52	1.5895276574862223
1102	53	1.5732914584092939
1102	54	1.63660788936469
1102	55	1.6629341365791817
1102	56	1.5583362486759853
1102	57	1.6442312265176682
1102	58	1.5354721317755775
1102	59	1.5846976718154338
1102	60	1.6132439111077517
1102	61	1.6060589645579526
1102	62	1.6074745088187612
1102	63	1.644857757893739
1102	64	1.6445443366186865
1102	65	1.6774049071205468
1102	66	1.7258834870556967
1102	67	1.7624229646459426
1102	68	1.7282017246668318
1102	69	1.7074098392005084
1102	70	1.7009747526233454
1102	71	1.6954765021645493
1102	72	1.6459229378495088
1102	73	1.718344111714849
1102	74	1.8071531826461467
1102	75	1.7395284172731351
1103	1	0.5468756497147886
1103	2	0.5461071521678953
1103	3	0.5918507049213027
1103	4	0.5487959779379636
1103	5	0.6125237674189208
1103	6	0.6719900810031234
1103	7	0.7072224230192603
1103	8	0.6734109330164486
1103	9	0.7758719546456305
1103	10	0.8359167213846561
1103	11	0.8428581263646464
1103	12	0.9040152688826311
1103	13	0.8526180364285949
1103	14	0.9117676208462342
1103	15	0.8992131162830788
1103	16	0.9899056225841463
1103	17	0.9010644762350566
1103	18	0.9677386253881366
1103	19	1.0843778993953848
1103	20	1.0998036908552749
1103	21	1.0187689062566676
1103	22	1.0126481830137095
1103	23	1.0214099041922182
1103	24	1.093314372948221
1103	25	1.1017493241692478
1103	26	1.1574986185804974
1103	27	1.0994171197820535
1103	28	1.0731344326959373
1103	29	1.1278676733004858
1103	30	1.1464451689739974
1103	31	1.1539219274409191
1103	32	1.1102159080526235
1103	33	1.143841280817277
1103	34	1.1779386442167095
1103	35	1.2149451441997883
1103	36	1.1683777868160234
1103	37	1.2016244062208727
1103	38	1.2105451308781454
1103	39	1.239668697983106
1103	40	1.1744208685402668
1103	41	1.1426754804843071
1103	42	1.1724707335919575
1103	43	1.1774867204091706
1103	44	1.185450925604357
1103	45	1.0779515890749423
1103	46	1.0907829273535867
1103	47	1.142912480184144
1103	48	1.1451325552406288
1103	49	1.1482996363277067
1103	50	1.0812799119225645
1103	51	1.0728273330870337
1103	52	1.0574653881933216
1103	53	1.066345124492237
1103	54	1.164366955537048
1103	55	1.0019174092030099
1103	56	1.0642769503332161
1103	57	1.0232971551613304
1103	58	1.0717418154189318
1103	59	1.0255362700351753
1103	60	1.0157433287839481
1103	61	1.0888364633263805
1103	62	1.0784610991900223
1103	63	1.1202286572054518
1103	64	1.1405826369443446
1103	65	1.1535070573037558
1103	66	1.2080062461078853
1103	67	1.138172744002155
1103	68	1.166487587123349
1103	69	1.159891717243724
1103	70	1.1513435843359616
1103	71	1.155243620586635
1103	72	1.1463028966413553
1103	73	1.1320206505179868
1103	74	1.1681295169267827
1103	75	1.134582580378936
1104	1	0.4124720013365497
1104	2	0.434469221133412
1104	3	0.37421959132830196
1104	4	0.4585980859932448
1104	5	0.4743769248483005
1104	6	0.4227032365710883
1104	7	0.5179564031951642
1104	8	0.5491008208660517
1104	9	0.5885051623332842
1104	10	0.6343159023609743
1104	11	0.5983981701079557
1104	12	0.5831604129098054
1104	13	0.617740889981576
1104	14	0.7486556798531723
1104	15	0.6367567432550203
1104	16	0.6643838439997509
1104	17	0.7163688179913095
1104	18	0.6352045381807301
1104	19	0.7425274177536139
1104	20	0.7651208298566203
1104	21	0.7269399472543867
1104	22	0.8033474942892411
1104	23	0.8091803914182449
1104	24	0.8244558872113288
1104	25	0.7746569745950467
1104	26	0.8090755881928544
1104	27	0.8534959508118831
1104	28	0.7611358520267615
1104	29	0.7237062418704525
1104	30	0.7956232368226637
1104	31	0.82336094275108
1104	32	0.8766568778511186
1104	33	0.800000245869164
1104	34	0.7455470717843014
1104	35	0.8373443949792545
1104	36	0.7895397766712975
1104	37	0.7973326755679864
1104	38	0.8566687289981161
1104	39	0.7857707734635824
1104	40	0.8330782048518728
1104	41	0.8508610638060077
1104	42	0.8296232302181394
1104	43	0.7778747096129521
1104	44	0.814052768407656
1104	45	0.832758699689677
1104	46	0.8998137105449899
1104	47	0.8898737161653472
1104	48	0.906374935062864
1104	49	0.7886638924587501
1104	50	0.7862690924335283
1104	51	0.8724422523480513
1104	52	0.8245020115820338
1104	53	0.7999093899303702
1104	54	0.861751082299584
1104	55	0.8624576856235038
1104	56	0.8782282742663909
1104	57	0.853025155533615
1104	58	0.8196989097462577
1104	59	0.7770835733066193
1104	60	0.7775063238046585
1104	61	0.7777011150086324
1104	62	0.8019329917862379
1104	63	0.8470092193535308
1104	64	0.851781971487604
1104	65	0.8244258995020886
1104	66	0.8390157160692624
1104	67	0.8214310389437571
1104	68	0.842625960244483
1104	69	0.7830000517404443
1104	70	0.8497189159009508
1104	71	0.8458841808732949
1104	72	0.8526503302120183
1104	73	0.7648607082219314
1104	74	0.8676339145255554
1104	75	0.9307434068482934
1105	1	0.40906140443985706
1105	2	0.4215483637256092
1105	3	0.4251526295733896
1105	4	0.5010211978602257
1105	5	0.43152244352408076
1105	6	0.47570020309396455
1105	7	0.536062422845184
1105	8	0.5795546035144099
1105	9	0.6670345829316418
1105	10	0.6401249359657442
1105	11	0.6911897199219297
1105	12	0.7469509077645355
1105	13	0.7355273714860004
1105	14	0.7329402128741478
1105	15	0.745384038542241
1105	16	0.7736603052684856
1105	17	0.7045299038860122
1105	18	0.7585762968090535
1105	19	0.7642178116175842
1105	20	0.7918054796364302
1105	21	0.7401157218610308
1105	22	0.786958880715936
1105	23	0.7823817985311479
1105	24	0.7399652898641236
1105	25	0.7982348914071977
1105	26	0.8749621575479765
1105	27	0.8355509438476503
1105	28	0.8158983266382833
1105	29	0.8038464463298141
1105	30	0.8933038751293267
1105	31	0.9059230483145839
1105	32	0.8865947065873101
1105	33	0.9008772511364498
1105	34	0.9559474776244983
1105	35	0.9481521427351183
1105	36	1.0228984515523791
1105	37	0.9760794703314843
1105	38	1.0887533810305072
1105	39	0.9796085068404636
1105	40	0.9690872771259649
1105	41	0.9280953435992245
1105	42	0.9327109570249945
1105	43	1.065951276753033
1105	44	1.0812485102767724
1105	45	1.096373868577011
1105	46	1.1108680221746923
1105	47	1.2634473624574056
1105	48	1.2111115988387127
1105	49	1.143812215599148
1105	50	1.1063521282383135
1105	51	1.0132555658586568
1105	52	1.070033827425945
1105	53	1.1606451489191087
1105	54	1.1197410376609156
1105	55	1.1161224488583628
1105	56	1.1056320608567702
1105	57	1.196570287891575
1105	58	1.114861677568875
1105	59	1.1061548337388913
1105	60	1.079574851104077
1105	61	1.0686864225423989
1105	62	1.1488402435986274
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2213	3	-0.49199302788987964
2213	4	-1.1685708421317074
2213	5	-0.676219517217632
2213	6	-0.7055400598204145
2213	7	-0.7550642940644039
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2213	11	-0.7622707158780244
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2213	39	-0.7662559356229863
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2213	41	-0.8291798109991007
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2213	46	-0.6665722052051564
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2213	73	-0.7035905440079233
2213	74	-0.8130481750057186
2213	75	-0.6811095843165695
2214	1	-0.5014925625942048
2214	2	-0.6226158155287109
2214	3	-0.5573495373528523
2214	4	-0.42943041552138794
2214	5	-0.5336602683390694
2214	6	-0.5418643655176467
2214	7	-0.6761969954367117
2214	8	-0.7947912726146811
2214	9	-0.818420256625835
2214	10	-0.7391743250569007
2214	11	-0.6542598766050887
2214	12	-0.6075249826893767
2214	13	-0.6629898081868504
2214	14	-0.8516618906450049
2214	15	-0.7649772369163585
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2214	17	-0.8326164420246087
2214	18	-0.7679906334157707
2214	19	-0.7812636030141888
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2214	21	-0.8428269912618518
2214	22	-0.7699855914424134
2214	23	-0.7676526294246955
2214	24	-0.8329760906308792
2214	25	-0.8718988433613646
2214	26	-0.7064618013682562
2214	27	-0.8320794584139293
2214	28	-0.876908786286954
2214	29	-0.8047473746509013
2214	30	-0.9094052544463835
2214	31	-0.9025664536062692
2214	32	-0.899414042100112
2214	33	-0.917186384323216
2214	34	-0.8557197523228837
2214	35	-0.7966311231912897
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2214	37	-0.8893689797773092
2214	38	-0.8488638878271857
2214	39	-0.8044815326472303
2214	40	-0.8682830494128524
2214	41	-0.8598281568122133
2214	42	-0.8483410741577515
2214	43	-0.9103426522167766
2214	44	-0.9525502902414971
2214	45	-0.8570828010838696
2214	46	-0.9136372506618073
2214	47	-1.0115020953705027
2214	48	-1.038445195124396
2214	49	-0.9350964342388579
2214	50	-0.8507050528284026
2214	51	-0.9145432057492116
2214	52	-0.7997516828057769
2214	53	-0.8885327771478302
2214	54	-0.8389320848844903
2214	55	-0.8283011366804516
2214	56	-0.7709064859356474
2214	57	-0.9197030563724411
2214	58	-0.8690823435622121
2214	59	-0.9255152197632626
2214	60	-0.8710752600947416
2214	61	-0.9190713971067694
2214	62	-0.9403646749521499
2214	63	-0.9905624997534659
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2214	69	-0.9689379863663063
2214	70	-0.9843192711083297
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2214	72	-1.096743984853429
2214	73	-1.158539358032865
2214	74	-1.1594089700852344
2214	75	-1.0520079771597146
2215	1	-1.1715058849029703
2215	2	-1.1413109521290394
2215	3	-0.939382599322574
2215	4	-2.0937836250771014
2215	5	-1.3020866610212778
2215	6	-1.188815555980284
2215	7	-1.0692397139435244
2215	8	-1.0242331342224986
2215	9	-1.195769871086057
2215	10	-1.2530189439100496
2215	11	-1.195427282478029
2215	12	-1.30944795384287
2215	13	-1.2788277984257945
2215	14	-1.3082270208442282
2215	15	-1.4246563509098067
2215	16	-1.3756547307300409
2215	17	-1.3952251033152052
2215	18	-1.4052310531602927
2215	19	-1.3950154574497518
2215	20	-1.4635529662698445
2215	21	-1.4420814877566528
2215	22	-1.3189469637992772
2215	23	-1.4186835685407218
2215	24	-1.5425558535996444
2215	25	-1.468952029456723
2215	26	-1.452721051220209
2215	27	-1.3761374208630244
2215	28	-1.287041498467019
2215	29	-1.5166257236014076
2215	30	-1.3995347149084054
2215	31	-1.4922230926475137
2215	32	-1.4460024728291856
2215	33	-1.5704446199871498
2215	34	-1.5629967515957688
2215	35	-1.6690377927700908
2215	36	-1.5788123647572903
2215	37	-1.499620982943327
2215	38	-1.6196237446550086
2215	39	-1.673918115770725
2215	40	-1.614935157703954
2215	41	-1.7384468190138591
2215	42	-1.694894132296234
2215	43	-1.652264744900478
2215	44	-1.6283983460885665
2215	45	-1.627497670710632
2215	46	-1.5008465157739153
2215	47	-1.6516911380848711
2215	48	-1.8133718810323103
2215	49	-1.8130561674369972
2215	50	-1.7726796490302945
2215	51	-1.769740989276393
2215	52	-1.6942066028364735
2215	53	-1.6056077808761486
2215	54	-1.8282292028788092
2215	55	-1.7227859906078322
2215	56	-1.8434585476318261
2215	57	-1.808206075230835
2215	58	-1.837777696713779
2215	59	-1.7556492481149206
2215	60	-1.6470304685601889
2215	61	-1.7164037388270756
2215	62	-1.6518518135525682
2215	63	-1.7062450215351639
2215	64	-1.611800400715797
2215	65	-1.5298437453720553
2215	66	-1.5798360315810207
2215	67	-1.6524898271612578
2215	68	-1.6584889245753658
2215	69	-1.5722866810395253
2215	70	-1.5384505072138381
2215	71	-1.5715008721313382
2215	72	-1.7864808705009239
2215	73	-1.6727166225829322
2215	74	-1.7062730916194617
2215	75	-1.6894268504547405
2216	1	-0.8667468111670225
2216	2	-0.7314374021022019
2216	3	-0.9223909292728756
2216	4	-0.807934287285228
2216	5	-0.8610129545733827
2216	6	-0.8502809339529449
2216	7	-0.9395190239721032
2216	8	-0.9752691431318965
2216	9	-1.462050583709349
2216	10	-1.6551499242576924
2216	11	-1.6548691594537388
2216	12	-1.4378350725465054
2216	13	-1.497835714395812
2216	14	-1.6078368340047646
2216	15	-1.4167863163483858
2216	16	-1.4433144530457938
2216	17	-1.8299702585273536
2216	18	-1.6663655515386822
2216	19	-1.9558932062820418
2216	20	-1.8534609462448728
2216	21	-1.9473635843759922
2216	22	-2.044216652233416
2216	23	-2.101223060207566
2216	24	-1.9586781490193133
2216	25	-1.9977007315595898
2216	26	-1.9436024219791577
2216	27	-2.0009685900190206
2216	28	-1.9191115906842526
2216	29	-1.990565287931254
2216	30	-1.9565125995108374
2216	31	-2.06557985386587
2216	32	-1.9397712037535442
2216	33	-1.8556557916186875
2216	34	-1.8325445334502817
2216	35	-1.8285393698073023
2216	36	-1.9393997341578952
2216	37	-1.847310589761463
2216	38	-2.016237579933822
2216	39	-1.876670651760719
2216	40	-1.9023002453452804
2216	41	-1.8019805271420424
2216	42	-1.7740391219162674
2216	43	-1.7977753275320296
2216	44	-1.9259730902128425
2216	45	-1.8559906574164486
2216	46	-1.6994926783915218
2216	47	-2.0387086313973697
2216	48	-1.9887633585539177
2216	49	-1.9864289699905342
2216	50	-1.9337441940399884
2216	51	-1.9210952355084032
2216	52	-1.9201033944411705
2216	53	-2.012551322110319
2216	54	-1.99746015834274
2216	55	-1.9742594124963873
2216	56	-2.08178372641936
2216	57	-1.979388125225288
2216	58	-1.9604835292502543
2216	59	-2.1337470455858885
2216	60	-2.038681029758404
2216	61	-1.9541735635444262
2216	62	-1.9599462584566325
2216	63	-1.9114041185514914
2216	64	-1.9282354580798042
2216	65	-1.811774740534485
2216	66	-1.7985964331964084
2216	67	-1.8554526401236195
2216	68	-1.9465569930671371
2216	69	-2.01450480567998
2216	70	-1.822159477150084
2216	71	-1.802634305897378
2216	72	-1.9037650363803564
2216	73	-1.989884562936421
2216	74	-1.9645436384774762
2216	75	-1.9731839604298393
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63521.0
3	24794.0
4	14458.0
5	9249.0
6	6942.0
7	6357.0
8	6232.0
9	6342.0
10	6934.0
11	7598.0
12	8078.0
13	8569.0
14	8782.0
15	9443.0
16	10019.0
17	10992.0
18	11446.0
19	12697.0
20	14810.0
21	17335.0
22	21641.0
23	26752.0
24	27789.0
25	28577.0
26	29306.0
27	30371.0
28	34648.0
29	41609.0
30	47989.0
31	53884.0
32	65168.0
33	81102.0
34	105700.0
35	143218.0
36	201828.0
37	314855.0
38	529398.0
39	786285.0
40	199226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.78993560746745	18.202682439461718	14.68649128975716	25.320890663313673
2	15.284385499464642	19.721390030222636	38.20252461852495	26.791699851787776
3	18.230639971468634	24.17917935404934	29.26268040584682	28.32750026863521
4	12.496431142420604	16.443018258357895	35.67494399575401	35.385606603467494
5	13.965960131097177	37.35230287749989	33.69688363772891	14.984853353674026
6	33.043093362850975	36.16619374449802	16.594604168597552	14.196108724053461
7	28.50488103939813	32.11954384066536	20.60900152749877	18.766573592437744
8	26.926665484174496	34.60055612299062	18.914280966733507	19.558497426101383
9	26.39819339665107	15.843493223753482	18.72484485219944	39.03346852739601
10	14.994012615708838	29.32799524174394	32.3640870359942	23.31390510655302
11	34.960221977616975	23.183896667138075	22.476321585358498	19.379559769886455
12	23.794226117393414	25.027527125396	29.424047076416354	21.754199680794233
13	28.367412245975192	21.89396938506202	25.603622327790976	24.134996041171814
14	22.30657287662406	21.085183022159992	26.851714143726173	29.756529957489775
15	23.74822224844939	28.828091672309537	23.876101678487576	23.547584400753497
16	23.319756977403987	28.224455970370947	25.3516603697565	23.104126682468564
17	22.247405931428368	27.989335587449304	26.656811502848743	23.106446978273585
18	22.158083549650257	26.87107819123229	28.2345835869027	22.736254672214752
19	23.1287516198163	26.654816858154696	28.49030648523186	21.72612503679715
20	23.24702385759744	25.87263531298901	28.565824559483456	22.314516269930092
21	23.48437542242177	25.694650961753396	28.541040108720235	22.279933507104598
22	23.09756486720634	25.42545924940364	28.555185750246913	22.921790133143098
23	23.02136929077584	25.55905498313506	28.59279936918001	22.826776356909086
24	22.66574181737501	25.63746852836543	28.516325688930856	23.180463965328702
25	22.986349354850514	25.562661798335444	28.545866253412335	22.90512259340171
26	22.547504873140095	26.05431935619159	28.702597337511442	22.69557843315687
27	22.744223631931916	25.945083744602453	28.387542609173284	22.92315001429235
28	22.767274818352437	25.884827070465672	28.44062047035145	22.907277640830443
29	22.84334048498911	25.914144515330772	28.47235092491157	22.77016407476855
30	22.80489344914439	25.887055532530066	28.52294080427423	22.785110214051308
31	22.87627228497337	25.62626058163294	28.470765714948826	23.02670141844486
32	22.722326590714825	25.91824403371716	28.352357554039127	23.00707182152889
33	22.82702789302717	25.83630622926912	28.2551479832986	23.08151789440511
34	23.046344342615914	25.846480553519974	28.280574683429972	22.82660042043414
35	22.961014765815666	25.906860256763547	28.265151804820675	22.86697317260011
36	23.051191062866824	25.86243895524676	28.056539432067467	23.029830549818946
37	22.997124634233895	25.589865316324843	28.3623864052522	23.05062364418906
38	23.084324117292823	25.75638366354338	28.1945840256543	22.96470819350949
39	23.180416360661315	25.958131875231565	28.028661878699257	22.832789885407863
40	23.101017339805736	25.666353946699594	28.421926702219608	22.810702011275062
41	22.73548974919024	25.90055953064563	28.167756346061168	23.19619437410297
42	23.150797682190916	25.781785902291144	27.989993826075978	23.077422589441966
43	22.987608521444862	25.744119972956376	28.02173115459203	23.246540351006736
44	23.040280374109038	25.97950133518544	27.813358183396232	23.166860107309287
45	23.1839406367077	25.960431064205412	27.627716318247053	23.22791198083983
46	23.13529701821557	25.885761109177395	27.55111592781276	23.42782594479427
47	23.078426654448727	26.082490877746977	27.450845312299137	23.38823715550516
48	23.01254774997849	26.291503214975688	27.353580741591898	23.342368293453923
49	23.257089460612644	26.052859268830105	27.204708123688775	23.485343146868477
50	23.36670141799253	26.281198324157685	27.12366802606646	23.228432231783323
51	23.127764412023836	26.434033329674644	27.002086112371877	23.436116145929642
52	23.443206419272585	26.27223896654563	26.918411636118165	23.366142978063618
53	23.168423675585313	26.353189116213088	27.14245879291114	23.33592841529046
54	23.09492960151196	26.407022149064762	27.168803105397828	23.32924514402545
55	23.145559527662765	26.27245681679298	27.027219445116472	23.55476421042778
56	23.01095543164928	26.530505281582776	27.014047411455817	23.444491875312124
57	23.165766601974518	26.24468662982027	26.92751402835204	23.662032739853178
58	23.051781858501442	26.130024367661974	27.237297783508502	23.580895990328084
59	22.989378320407976	26.436422046866188	27.135205480039065	23.438994152686767
60	22.996883876781617	26.45029595589568	27.277153022283546	23.275667145039154
61	23.046419215297984	26.203658901564843	27.521526634224546	23.228395248912626
62	22.970460240010553	26.388599498879074	27.179711196096534	23.461229065013846
63	22.780316685104143	26.257418392735417	27.462293270606352	23.499971651554088
64	23.130730599402362	26.109529750906923	27.284270694437808	23.47546895525291
65	23.02795782014254	26.1800407530714	27.231009784099708	23.560991642686353
66	22.963222541518498	26.272537776829815	27.126857688401195	23.637381993250493
67	23.02699245740809	26.210276122158344	27.067322907326336	23.695408513107232
68	23.04384547373168	26.27197762529892	27.35929605666992	23.324880844299482
69	23.126567374352945	26.229869705208365	27.026478243327702	23.617084677110995
70	22.918474290642198	26.103101157494706	27.21750957985764	23.76091497200545
71	22.980399887002932	26.17919265110309	27.125032180101773	23.71537528179221
72	22.858323990153647	26.45665418455222	26.938521602936895	23.746500222357238
73	22.9964353970936	26.20434727288429	27.06121663252814	23.738000697493966
74	22.89312683667542	26.37348870912336	26.944675396774638	23.78870905742658
75	22.975282012878456	26.40469177666795	26.81406530991093	23.805960900542665
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1153.0
1	794.0
2	595.5
3	753.5
4	751.0
5	571.5
6	528.5
7	1142.0
8	1619.0
9	2947.5
10	5015.5
11	5832.5
12	5910.0
13	5271.0
14	4660.0
15	5127.0
16	5566.0
17	5970.0
18	6963.0
19	8143.0
20	8734.0
21	9809.5
22	12533.5
23	15796.0
24	17410.0
25	19600.5
26	25153.5
27	30725.0
28	32934.0
29	36919.5
30	44239.0
31	51544.5
32	55516.0
33	60079.5
34	69559.0
35	79597.5
36	84720.0
37	87172.5
38	92450.0
39	97873.0
40	100471.0
41	105816.0
42	114224.0
43	120479.5
44	123672.0
45	127820.0
46	134494.0
47	140720.5
48	144421.0
49	147304.5
50	151117.5
51	151167.5
52	150288.0
53	146720.5
54	141720.5
55	135758.5
56	131229.0
57	126822.5
58	113171.5
59	95247.0
60	86567.0
61	80617.5
62	65216.5
63	48663.0
64	41561.0
65	33934.0
66	21854.5
67	15331.5
68	13261.0
69	10828.0
70	6542.0
71	3713.0
72	2737.0
73	2459.0
74	1824.0
75	1081.5
76	696.0
77	583.0
78	345.0
79	183.5
80	147.0
81	117.0
82	61.0
83	27.5
84	20.0
85	16.5
86	9.0
87	4.5
88	4.0
89	2.0
90	0.5
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07336984466423903
2	0.015029941225019314
3	0.0029664357680959175
4	0.14051017421547662
5	0.09591475650176799
6	0.06872242862755543
7	0.0290381101299167
8	0.026434238733476955
9	0.0498690813014347
10	0.029664357680959177
11	0.015985792750294665
12	0.10867043030458043
13	0.0904433305295022
14	0.049341714942662096
15	0.020369525607591966
16	0.03213638748770577
17	0.022347149452989244
18	0.019314792890046752
19	0.013876327315204235
20	0.013283040161585054
21	0.027620813040715318
22	0.01700756507041659
23	0.014238891686860404
24	0.021654981107100197
25	0.015194743212135754
26	0.03269671424390167
27	0.02811521900206464
28	0.03783853624193459
29	0.0509567744164032
30	0.03536650643518799
31	0.02050136719728512
32	0.01170094108526723
33	0.014041129302320675
34	0.013612644135817932
35	0.005932871536191835
36	0.010184762803795983
37	0.008009376573858976
38	0.0022083466273602943
39	0.007514970612509657
40	0.011404297508457638
41	0.01087693114968503
42	0.00715240624085349
43	0.010778049957415166
44	0.009327792470790495
45	0.004944059613493195
46	0.0018457822557041265
47	0.005075901203186348
48	0.005702148754228819
49	0.0012854554995082308
50	2.3072278196301581E-4
51	0.002340188217053446
52	0.002636831793863038
53	0.0
54	3.296039742328797E-5
55	3.2960397423287973E-4
56	0.009525554855330224
57	0.0029005149732493412
58	1.6480198711643987E-4
59	0.008009376573858976
60	0.0015161782814712467
61	9.228911278520633E-4
62	0.02452253568292625
63	0.008833386509441177
64	0.011173574726494622
65	0.018227099775078248
66	0.01769973341630564
67	0.016612040301337137
68	0.01707348586526317
69	0.006855762664043898
70	0.016348357121950836
71	0.007811614189319249
72	0.01769973341630564
73	0.007449049817663082
74	0.02010584242820566
75	0.001318415896931519
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	3033944.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.22237746775595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.45158968683685	41.113937887609914
2	13.061202396686951	14.42541297865211
3	5.108334742392369	8.462831681281298
4	2.516582419395548	5.558866571703178
5	1.372745961774385	3.7903147834221382
6	0.8756075365090494	2.901187793682878
7	0.5658164541699164	2.1872010866746856
8	0.3787782343231527	1.6733627705890604
9	0.27448199587466443	1.3641793545864376
>10	1.2951633887163312	12.355237177299426
>50	0.06822447991810178	2.5644182898297325
>100	0.030094710233221533	2.8797731271452602
>500	0.0011383421835911352	0.4168409768292798
>1k	2.3965098585193296E-4	0.30643552069460295
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3409	0.1123619948159887	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.592079484657595E-5
2	0.0	0.0	0.0	0.0	6.592079484657595E-5
3	0.0	0.0	0.0	0.0	9.888119226986391E-5
4	0.0	0.0	0.0	0.0	9.888119226986391E-5
5	0.0	0.0	0.0	0.0	9.888119226986391E-5
6	0.0	0.0	0.0	0.0	9.888119226986391E-5
7	0.0	0.0	0.0	0.0	9.888119226986391E-5
8	0.0	0.0	0.0	0.0	9.888119226986391E-5
9	0.0	0.0	0.0	0.0	9.888119226986391E-5
10	0.0	0.0	0.0	0.0	1.318415896931519E-4
11	0.0	0.0	0.0	3.2960397423287973E-5	1.318415896931519E-4
12	0.0	0.0	0.0	3.2960397423287973E-5	3.2960397423287973E-4
13	0.0	0.0	0.0	3.2960397423287973E-5	3.625643716561677E-4
14	0.0	0.0	0.0	3.2960397423287973E-5	3.625643716561677E-4
15	0.0	0.0	0.0	3.2960397423287973E-5	3.9552476907945563E-4
16	0.0	0.0	0.0	3.2960397423287973E-5	3.9552476907945563E-4
17	0.0	0.0	0.0	3.2960397423287973E-5	4.284851665027436E-4
18	0.0	0.0	0.0	3.2960397423287973E-5	4.284851665027436E-4
19	0.0	0.0	0.0	3.2960397423287973E-5	4.284851665027436E-4
20	0.0	0.0	0.0	3.2960397423287973E-5	4.944059613493195E-4
21	0.0	0.0	0.0	3.2960397423287973E-5	5.603267561958955E-4
22	0.0	0.0	0.0	1.9776238453972781E-4	5.603267561958955E-4
23	0.0	0.0	0.0	2.307227819630158E-4	5.603267561958955E-4
24	0.0	0.0	0.0	2.636831793863038E-4	5.603267561958955E-4
25	0.0	0.0	0.0	3.2960397423287973E-4	5.603267561958955E-4
26	0.0	0.0	0.0	5.273663587726076E-4	5.603267561958955E-4
27	0.0	0.0	0.0	6.921683458890474E-4	6.592079484657595E-4
28	0.0	0.0	0.0	0.0014172970892013827	6.592079484657595E-4
29	0.0	0.0	0.0	0.002340188217053446	6.592079484657595E-4
30	0.0	0.0	0.0	0.0039882080882178445	6.592079484657595E-4
31	0.0	0.0	0.0	0.00731720822796993	6.921683458890474E-4
32	0.0	0.0	0.0	0.01236014903373299	6.921683458890474E-4
33	0.0	0.0	0.0	0.018952228518390582	6.921683458890474E-4
34	0.0	0.0	0.0	0.026401278336053664	7.251287433123354E-4
35	0.0	3.2960397423287973E-5	0.0	0.0367178827295428	7.251287433123354E-4
36	0.0	3.2960397423287973E-5	0.0	0.049605398122048394	7.251287433123354E-4
37	0.0	3.2960397423287973E-5	0.0	0.06522862650068689	7.251287433123354E-4
38	0.0	3.2960397423287973E-5	0.0	0.08259875594275966	8.569703330054872E-4
39	0.0	3.2960397423287973E-5	0.0	0.10323196472973792	8.569703330054872E-4
40	0.0	3.2960397423287973E-5	0.0	0.1224808368249381	8.569703330054872E-4
41	0.0	3.2960397423287973E-5	0.0	0.13935656030566154	8.569703330054872E-4
42	0.0	3.2960397423287973E-5	0.0	0.15883615518282473	8.569703330054872E-4
43	0.0	3.2960397423287973E-5	0.0	0.18171067099458657	8.569703330054872E-4
44	0.0	3.2960397423287973E-5	0.0	0.2071560978053649	8.569703330054872E-4
45	0.0	3.2960397423287973E-5	0.0	0.23494171283319665	8.569703330054872E-4
46	0.0	3.2960397423287973E-5	0.0	0.2660233676033572	8.569703330054872E-4
47	0.0	3.2960397423287973E-5	0.0	0.29931336900087807	8.899307304287752E-4
48	0.0	3.2960397423287973E-5	0.0	0.331581598078277	8.899307304287752E-4
49	0.0	3.2960397423287973E-5	0.0	0.36655257974438554	8.899307304287752E-4
50	0.0	3.2960397423287973E-5	0.0	0.4019520465769968	9.228911278520631E-4
51	0.0	3.2960397423287973E-5	0.0	0.4373844738070314	9.228911278520631E-4
52	0.0	3.2960397423287973E-5	0.0	0.47268505944737277	9.558515252753512E-4
53	0.0	3.2960397423287973E-5	0.0	0.5097984669459951	9.558515252753512E-4
54	0.0	3.2960397423287973E-5	0.0	0.5520866568400735	9.558515252753512E-4
55	0.0	3.2960397423287973E-5	0.0	0.6001429162832274	9.558515252753512E-4
56	0.0	3.2960397423287973E-5	0.0	0.6496823936104292	9.88811922698639E-4
57	0.0	3.2960397423287973E-5	0.0	0.7015620591546845	9.88811922698639E-4
58	0.0	3.2960397423287973E-5	0.0	0.7556830317237233	9.88811922698639E-4
59	0.0	3.2960397423287973E-5	0.0	0.814022935162943	9.88811922698639E-4
60	0.0	3.2960397423287973E-5	0.0	0.8751644723831422	0.001021772320121927
61	0.0	3.2960397423287973E-5	0.0	0.935679762052299	0.001021772320121927
62	0.0	3.2960397423287973E-5	0.0	0.9963928141059953	0.001087693114968503
63	0.0	3.2960397423287973E-5	0.0	1.0589846088128192	0.001120653512391791
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4250	0.0	41.84584	1
GTATCAA	8520	0.0	34.234665	1
GTGGTAT	1865	0.0	26.344357	1
TATCAAC	11630	0.0	25.03732	2
ATCAACG	12100	0.0	23.981434	3
TCAACGC	12135	0.0	23.911474	4
TGGTATC	1970	0.0	23.698618	2
CAACGCA	12325	0.0	23.364315	5
AACGCAG	12715	0.0	22.644686	6
GCACCGT	700	0.0	19.727793	6
TATATCG	105	6.0344697E-4	19.71023	66
ACGCAGA	14770	0.0	19.308653	7
CGTGCCG	435	0.0	19.049726	10
CGAATCG	385	0.0	18.81152	37
GTACATG	9930	0.0	18.780952	1
CGCAGAG	15135	0.0	18.683622	8
AGAGTAC	14710	0.0	18.378746	11
GAGTACT	10175	0.0	18.018843	12
TACATGG	10155	0.0	18.014874	2
TACCGAT	505	0.0	17.776014	7
>>END_MODULE