FastQCFastQC Report
Mon 6 Feb 2017
SRR2077520_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077520_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826150
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56450.032.892771
GGTATCA30950.032.3384741
TCAACGC71850.024.9202964
TATCAAC73000.024.8068752
ATCAACG73000.024.5736683
CAACGCA73100.024.4959015
AACGCAG76350.023.6585626
ACGCAGA87100.020.5831997
GTACATG77750.020.2416821
CGCAGAG89600.019.8179048
TACATGG76700.019.562732
AGAGTAC87200.018.58600611
GTGGTAT18000.018.4069081
ACATGGG81000.018.1432083
GAGTACT52300.017.60505312
CAGAGTA94900.017.36804610
GCAGAGT98050.017.3018049
GTGTAGC12450.017.1871741
AGTACTT56000.016.68966313
GTACTTT56200.016.6308614
Produced by FastQC (version 0.11.5)