FastQCFastQC Report
Mon 6 Feb 2017
SRR2077520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826150
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34300.035.204821
GTATCAA64650.032.967271
TATCAAC89650.023.5675832
TCAACGC90050.023.3873394
ATCAACG90300.023.2459963
CAACGCA91750.022.8769345
AACGCAG94550.022.238736
ACGCAGA112000.018.5587227
GTACATG77550.018.3371071
CGCAGAG114900.018.061548
TACATGG76950.017.9303342
CGAATCG1552.254921E-517.8000137
AGAGTAC109950.017.77580311
GAGTACT74700.017.42724612
CGCGCCG1200.001482663317.24101447
AGTACTT77250.017.11229513
GTGGTAT18300.017.0161861
ACATGGG79150.016.9522783
GTACTTT77750.016.95065114
CAGAGTA119750.016.43642610
Produced by FastQC (version 0.11.5)