FastQCFastQC Report
Mon 6 Feb 2017
SRR2077519_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077519_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3668530
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31400.036.8391381
GTATCAA60850.033.5367661
TCAACGC76250.026.1945924
TATCAAC77750.026.0441742
CAACGCA76950.025.869125
ATCAACG77800.025.8511413
AACGCAG81600.024.3343966
GCGATAC5900.021.62472714
ACGCAGA96100.020.5209057
CGCAGAG100400.019.540078
TGCGATA6450.019.24640713
AGAGTAC98200.018.96092411
GAGTACT54900.018.59124412
GTACATG92800.018.4928021
CGATACT6950.018.35843315
CGCGAAC1150.001114808617.99046712
GGTGCGA6950.017.86055211
CAGAGTA108300.017.41479310
TACATGG94100.017.3764722
TCACGTC7750.017.36537646
Produced by FastQC (version 0.11.5)