FastQCFastQC Report
Mon 6 Feb 2017
SRR2077519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3668530
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36750.039.7334141
GTATCAA71500.033.1501351
TATCAAC100600.023.2711732
ATCAACG101350.023.1349813
TCAACGC102200.022.8748914
CAACGCA103000.022.6302365
AACGCAG107000.021.7128836
CGTACAC5300.020.880333
CGAATCG4900.020.41115437
GTACATG86250.018.8956831
ACGCAGA123550.018.7756487
GTGGTAT18350.018.6680891
TACATGG86650.018.3177342
CGCAGAG128800.017.9845168
CGCACGA5450.017.7185135
TATACTG12300.017.6871555
GAGTACT88450.017.68089912
GCGCGTT2551.375156E-917.58202236
CGCGTTG2751.9826984E-1017.55743237
AACGACT6100.017.52802719
Produced by FastQC (version 0.11.5)