FastQCFastQC Report
Mon 6 Feb 2017
SRR2077518_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077518_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3910392
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36600.039.6215321
GTATCAA73650.034.175731
TCAACGC92700.026.4989814
TATCAAC94600.026.256842
CAACGCA93750.026.2389955
ATCAACG94100.026.1773933
AACGCAG98950.024.8739156
ACGCAGA113650.021.3874637
GTACATG107750.020.8285541
CGCAGAG116600.020.6108578
TACATGG110850.019.9802282
AGAGTAC115600.019.32938211
GAGTACT67500.019.2086112
GTGGTAT17800.019.0094321
ACATGGG115400.018.4457863
CAGAGTA124400.018.18308410
TGGTATC17700.017.9314612
GCAGAGT131500.017.64719
GTACTTT73800.017.47875814
AGTACTT74600.017.42912713
Produced by FastQC (version 0.11.5)