FastQCFastQC Report
Mon 6 Feb 2017
SRR2077518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3910392
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45300.039.706991
GTATCAA88800.034.746711
TCAACGC120500.025.2197554
ATCAACG120900.025.1645873
TATCAAC121800.025.1466982
CAACGCA122150.024.873275
AACGCAG128050.023.7523546
GTGGTAT18900.020.681331
ACGCAGA148600.020.3274527
ATGCGCG1901.6087142E-819.96586851
GTACATG102750.019.6604211
CGCAGAG153650.019.5025738
TACATGG104600.019.3338642
GAGTACT106450.019.16715812
AGAGTAC151800.018.92201811
AGTACTT112100.018.31890113
GTACTTT114000.017.85421414
CAGAGTA162750.017.75498610
ACATGGG109350.017.7368933
CGCACGA3350.017.50150735
Produced by FastQC (version 0.11.5)