FastQCFastQC Report
Mon 6 Feb 2017
SRR2077517_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077517_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3814961
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33200.037.426611
GTATCAA64900.033.7179071
TCAACGC79550.026.577314
ATCAACG81300.026.1324983
TATCAAC83600.025.7439272
CAACGCA82700.025.608725
AACGCAG86800.024.4202236
ACGCAGA99650.021.1350737
GTACATG94200.020.592141
CGCAGAG103600.020.1333168
TACATGG95250.019.6621652
TCGCGAG900.00574529619.16175823
AGAGTAC100650.019.01248211
GAGTACT60500.018.35173812
ACATGGG97850.018.3287683
CAGAGTA108750.017.94419310
GTGGTAT17800.017.6456411
GCAGAGT116850.017.2604689
AGTACTT66400.016.82671213
GTACTTT68000.016.12630514
Produced by FastQC (version 0.11.5)