FastQCFastQC Report
Mon 6 Feb 2017
SRR2077517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3814961
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38250.039.903721
GTATCAA80900.035.637131
TATCAAC109150.026.1768252
TCAACGC109050.026.1391054
ATCAACG109100.026.127473
CAACGCA111800.025.420755
AACGCAG117150.024.3136866
ACGCAGA138000.020.4645397
CGCAGAG141150.019.8132028
AGAGTAC140050.018.93389711
GAGTACT100000.018.71376612
GTACATG91300.018.5372091
GTGGTAT19500.017.9263971
CAGAGTA151300.017.84554310
AGTACTT105900.017.69587513
GCAGAGT156150.017.5121489
TACATGG92950.017.3791352
GTACTTT107850.017.30329714
ACATGGG95800.015.888473
TACTTTT121150.015.54506315
Produced by FastQC (version 0.11.5)