FastQCFastQC Report
Mon 6 Feb 2017
SRR2077516_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077516_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3532667
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36550.039.7541471
GTATCAA71800.034.51341
TATCAAC96000.025.4014572
TCAACGC96900.025.061594
ATCAACG96500.025.0217763
CAACGCA98950.024.6504545
AACGCAG102100.023.8740186
ACGCAGA118450.020.317527
CGCAGAG120350.019.7400428
CGAATCG2354.5838533E-1019.0900837
AGAGTAC116800.018.98258611
GTACATG99150.018.9362471
GAGTACT72200.018.24372112
TACATGG101400.018.1640932
CGTATAC2105.0551535E-818.0684473
CAGAGTA125600.017.89942610
GTGGTAT17750.017.8886341
GCAGAGT131850.017.5739849
AGTACTT75750.017.39021313
GTACTTT75850.017.09450114
Produced by FastQC (version 0.11.5)