FastQCFastQC Report
Mon 6 Feb 2017
SRR2077516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3532667
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45590.12905263926659377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47900.042.6061361
GTATCAA96900.034.8759841
TATCAAC133300.025.2840462
ATCAACG136150.024.657423
TCAACGC137950.024.334654
CAACGCA139900.023.9929335
GTGGTAT21700.023.7510151
AACGCAG143800.023.409956
TGGTATC21400.022.2914032
ACGCAGA167500.019.912387
CGCAGAG171200.019.4024628
GAGTACT124600.018.73435812
AGAGTAC165100.018.67731911
GTACATG95700.018.3976151
AGTACTT129500.018.0708813
GTACTTT130700.017.86944214
CAGAGTA177200.017.67451710
TACATGG96650.017.6326312
GCAGAGT183600.017.433829
ACATGGG99600.016.6620313
Produced by FastQC (version 0.11.5)