FastQCFastQC Report
Mon 6 Feb 2017
SRR2077515_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077515_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3030698
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35250.038.782721
GTATCAA72500.034.284411
TCAACGC93950.026.1468564
ATCAACG94350.025.9254463
TATCAAC95600.025.8012832
GTGGTAT15550.025.7531151
CAACGCA96150.025.583635
AACGCAG99250.024.7971536
ACGCAGA113800.021.325917
GTACATG98250.020.6959271
TACATGG98950.020.3954472
CGCAGAG118850.020.3057568
AGAGTAC113900.019.49510211
TGGTATC18800.019.4509662
ACATGGG102600.019.43583
GAGTACT69700.018.7984212
CAGAGTA121600.018.37343810
GCAGAGT125900.018.2662379
AGTACTT75250.017.50620513
GTACTTT74450.017.27743514
Produced by FastQC (version 0.11.5)