FastQCFastQC Report
Mon 6 Feb 2017
SRR2077515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3030698
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49630.1637576558271395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33010.1089188035231488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32340.10670809166733208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47250.044.1727521
GTATCAA94550.034.667891
GTGGTAT17900.025.7180211
TATCAAC131800.024.5917742
TCAACGC132400.024.3267964
ATCAACG132650.024.2809453
TGGTATC19400.024.07122
CAACGCA135700.023.8200875
AACGCAG140850.023.0453056
ACGCAGA162100.019.8118827
CGCAGAG165600.019.4358238
GAGTACT121800.018.6285812
AGAGTAC166450.018.54878411
GTACATG92350.018.4402661
CAGAGTA172350.018.174310
GCAGAGT175350.018.0006339
AGTACTT126900.017.92279213
TACATGG94950.017.741882
GTACTTT129500.017.47405214
CCGAACG1200.001481180517.2436537
Produced by FastQC (version 0.11.5)