FastQCFastQC Report
Mon 6 Feb 2017
SRR2077514_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077514_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3292397
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51550.034.7516981
GGTATCA26200.033.9903561
TCAACGC64650.027.2662334
ATCAACG65000.027.0663453
TATCAAC66650.026.498192
CAACGCA67250.026.1615775
AACGCAG68900.025.6645686
GTACATG101400.021.3435631
ACGCAGA82100.021.3292797
CGCAGAG85950.020.1757938
TACATGG105050.019.9314562
AGAGTAC81300.019.29636811
ACATGGG109900.018.6449643
CGCGCTA1150.001109459818.00328836
GAGTACT35400.017.92183512
CAGAGTA92150.017.43487710
CCGGTAT3205.456968E-1217.25788158
GCAGAGT98200.016.9926939
CATGGGG81950.016.7956474
GTATAAC5150.016.755991
Produced by FastQC (version 0.11.5)