FastQCFastQC Report
Mon 6 Feb 2017
SRR2077514_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077514_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3292397
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52000.036.7190321
GGTATCA26350.035.4436651
TATCAAC73250.025.8168742
ATCAACG75700.024.755993
TCAACGC76650.024.4491624
CAACGCA77400.024.2266715
AACGCAG81300.023.019236
CGAATCG2700.020.43698137
GTACATG97250.019.313711
ACGCAGA97000.019.1134177
CGTACAC3850.018.8597283
TACATGG99750.018.2997422
CGCAGAG101450.018.2409958
TATACCG1954.553567E-717.7092535
GCGATAC2751.9826984E-1017.5633614
AGAGTAC97650.017.50203511
ACATGGG104650.017.2137453
GAGTACT50450.016.76719912
CGTATAC1704.8725462E-516.271143
CAGAGTA108800.016.0257410
Produced by FastQC (version 0.11.5)