FastQCFastQC Report
Mon 6 Feb 2017
SRR2077513_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077513_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162805
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27700.035.8974381
GTATCAA55450.032.627251
TATCAAC71350.024.6625442
TCAACGC70850.024.5451764
ATCAACG71250.024.455033
CAACGCA72300.024.0051885
AACGCAG75900.022.848146
GTGGTAT15800.019.8854351
ACGCAGA87250.019.7598767
CGCAGAG90550.018.9650928
AGAGTAC85200.018.61914611
GTACATG86200.018.5849531
TACATGG87450.018.0703832
GTATAGC9450.017.9025551
TGGTATC16350.017.5154782
CAGAGTA95150.017.07025110
GAGTACT47000.016.72959512
GCAGAGT99850.016.6118479
ACATGGG92050.016.6051043
GCGATAC3157.8216544E-1116.42367714
Produced by FastQC (version 0.11.5)