FastQCFastQC Report
Mon 6 Feb 2017
SRR2077513_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077513_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162805
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34300.038.617461
GTATCAA65200.033.6295241
TATCAAC87900.024.8522832
TCAACGC89250.024.443064
ATCAACG90150.024.1606883
CAACGCA90100.024.108975
AACGCAG95200.022.7405456
TATAGCG1103.244141E-521.9764825
GTACATG83900.019.7447931
GTGGTAT17200.019.5039391
ACGCAGA111450.019.1757977
TACATGG86250.018.434732
CGCAGAG115950.018.3428368
GCGATAC3600.018.20692314
AGAGTAC110550.017.9901211
TGGTATC18000.017.6666182
GAGTACT71750.017.46847512
TACGCTA4350.017.44333631
ACATGGG90350.017.4107573
CAGAGTA118800.017.0023810
Produced by FastQC (version 0.11.5)