##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077513_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3162805 Sequences flagged as poor quality 0 Sequence length 75 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.091505167090606 33.0 31.0 34.0 28.0 34.0 2 31.157453589456196 33.0 31.0 34.0 28.0 34.0 3 31.247245087825522 33.0 31.0 34.0 28.0 34.0 4 34.612407024777056 37.0 35.0 37.0 32.0 37.0 5 34.3496696761261 37.0 35.0 37.0 30.0 37.0 6 34.342079894271066 37.0 35.0 37.0 30.0 37.0 7 34.18551254345431 37.0 35.0 37.0 30.0 37.0 8 34.139305142112775 37.0 35.0 37.0 30.0 37.0 9 35.639480144997876 39.0 37.0 39.0 30.0 39.0 10 35.53047405704746 39.0 37.0 39.0 30.0 39.0 11 35.542492186524306 39.0 37.0 39.0 30.0 39.0 12 35.43551847173632 39.0 37.0 39.0 29.0 39.0 13 35.387267314930895 39.0 35.0 39.0 29.0 39.0 14 36.65399700582236 40.0 37.0 41.0 29.0 41.0 15 36.699598931960715 40.0 37.0 41.0 30.0 41.0 16 36.677612119621664 40.0 37.0 41.0 30.0 41.0 17 36.66620768589907 40.0 37.0 41.0 29.0 41.0 18 36.66324354489132 40.0 37.0 41.0 30.0 41.0 19 36.69554714881252 40.0 37.0 41.0 29.0 41.0 20 36.66197631532769 40.0 37.0 41.0 29.0 41.0 21 36.63669653993844 40.0 37.0 41.0 29.0 41.0 22 36.61743768585164 40.0 37.0 41.0 29.0 41.0 23 36.58644367894954 40.0 37.0 41.0 29.0 41.0 24 36.570114502791036 40.0 37.0 41.0 29.0 41.0 25 36.540685878516065 40.0 37.0 41.0 29.0 41.0 26 36.47320337485239 40.0 37.0 41.0 29.0 41.0 27 36.42136331515854 40.0 37.0 41.0 29.0 41.0 28 36.402702664248984 40.0 37.0 41.0 29.0 41.0 29 36.35332876987358 40.0 37.0 41.0 29.0 41.0 30 36.284425375576426 40.0 36.0 41.0 28.0 41.0 31 36.208749828079824 40.0 36.0 41.0 28.0 41.0 32 36.14332878568233 40.0 36.0 41.0 28.0 41.0 33 36.09079219237354 40.0 36.0 41.0 28.0 41.0 34 36.037449036535605 40.0 36.0 41.0 27.0 41.0 35 35.962200009169074 40.0 36.0 41.0 27.0 41.0 36 35.908864757707164 40.0 36.0 41.0 27.0 41.0 37 35.85159534021225 40.0 35.0 41.0 27.0 41.0 38 35.80296256013254 40.0 35.0 41.0 27.0 41.0 39 35.734784787554084 40.0 35.0 41.0 26.0 41.0 40 35.67441527378387 40.0 35.0 41.0 26.0 41.0 41 35.63295113040481 40.0 35.0 41.0 26.0 41.0 42 35.57677220062571 40.0 35.0 41.0 26.0 41.0 43 35.484732065366025 40.0 35.0 41.0 26.0 41.0 44 35.36868412690634 40.0 35.0 41.0 25.0 41.0 45 35.28197754841035 40.0 35.0 41.0 24.0 41.0 46 35.164218154454666 40.0 35.0 41.0 24.0 41.0 47 35.02487728456228 39.0 35.0 41.0 24.0 41.0 48 34.93717854878818 39.0 35.0 41.0 23.0 41.0 49 34.84728966850628 39.0 35.0 41.0 23.0 41.0 50 34.850827351038085 39.0 35.0 41.0 23.0 41.0 51 34.77665426733548 39.0 35.0 41.0 23.0 41.0 52 34.711295195245995 39.0 35.0 41.0 23.0 41.0 53 34.61255784027153 39.0 35.0 41.0 22.0 41.0 54 34.57523337670201 39.0 34.0 41.0 22.0 41.0 55 34.4930253366869 39.0 34.0 41.0 22.0 41.0 56 34.363306937987005 39.0 34.0 41.0 22.0 41.0 57 34.284245155803156 39.0 34.0 41.0 21.0 41.0 58 34.181668803482985 39.0 34.0 41.0 21.0 41.0 59 34.08902129596987 38.0 34.0 41.0 21.0 41.0 60 34.05661778073577 38.0 34.0 41.0 21.0 41.0 61 33.93917076772043 38.0 34.0 41.0 20.0 41.0 62 33.786143312660755 38.0 34.0 40.0 20.0 41.0 63 33.64858345677334 37.0 34.0 40.0 19.0 41.0 64 33.49197184145086 37.0 34.0 40.0 18.0 41.0 65 33.35219085590165 37.0 34.0 40.0 18.0 41.0 66 33.151931592368165 37.0 34.0 40.0 13.0 41.0 67 32.958466614287005 36.0 33.0 39.0 12.0 41.0 68 32.74603492785676 36.0 33.0 39.0 8.0 41.0 69 32.57557421339602 36.0 33.0 39.0 6.0 41.0 70 32.38157489949586 36.0 33.0 39.0 2.0 41.0 71 32.200410079027954 35.0 33.0 39.0 2.0 41.0 72 32.00231882774942 35.0 33.0 39.0 2.0 40.0 73 31.801337736597734 35.0 33.0 38.0 2.0 40.0 74 31.54366867385122 35.0 33.0 37.0 2.0 40.0 75 31.328446742685685 35.0 33.0 37.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2719236173275661 1101 2 0.2784775059701481 1101 3 0.3553245882979681 1101 4 0.22344899456285106 1101 5 0.396620459278914 1101 6 0.31099275210901567 1101 7 0.3632301943587848 1101 8 0.4391705482654089 1101 9 0.4990813149855029 1101 10 0.4244894387156677 1101 11 0.4548963612871546 1101 12 0.4779601617867755 1101 13 0.5459397199428153 1101 14 0.5458180714129739 1101 15 0.5035268810767803 1101 16 0.4825747943133507 1101 17 0.549502860519766 1101 18 0.5433582974113875 1101 19 0.60990154593852 1101 20 0.6223513248345256 1101 21 0.5412380661708625 1101 22 0.5685062275843293 1101 23 0.5288380486059907 1101 24 0.5910035638799442 1101 25 0.6787021844253118 1101 26 0.6619541890558551 1101 27 0.6235549730722951 1101 28 0.6660994909595814 1101 29 0.6533559557324935 1101 30 0.5927398380663291 1101 31 0.6574263909937557 1101 32 0.5263174528238963 1101 33 0.48062251397483635 1101 34 0.5184723351828779 1101 35 0.6883765109285065 1101 36 0.6082486637632769 1101 37 0.6406851072915245 1101 38 0.7076830023737841 1101 39 0.7225366946597589 1101 40 0.6878998884291505 1101 41 0.7394971456627246 1101 42 0.7502590490871981 1101 43 0.7066706960375768 1101 44 0.7634290659644236 1101 45 0.7779224489836807 1101 46 0.7704486532451185 1101 47 0.8331172039621109 1101 48 0.8831292863731122 1101 49 0.734909709092598 1101 50 0.6935562003344415 1101 51 0.6176496050008424 1101 52 0.6444170150280115 1101 53 0.6687691197755683 1101 54 0.7012584632063081 1101 55 0.7105054514887215 1101 56 0.5899447344199586 1101 57 0.6719676908960253 1101 58 0.698990298912733 1101 59 0.6776965558775458 1101 60 0.6671254077643098 1101 61 0.6415143386317084 1101 62 0.6136589890864101 1101 63 0.6163424601117242 1101 64 0.6782629623225915 1101 65 0.6276332753371392 1101 66 0.7006649314412954 1101 67 0.6363282112899853 1101 68 0.7701638426328472 1101 69 0.776140845626891 1101 70 0.6571703378697009 1101 71 0.6996788466798378 1101 72 0.6755443312340788 1101 73 0.7174610531235501 1101 74 0.6762258238337182 1101 75 0.6822637168347825 1102 1 0.6976395641254314 1102 2 0.7082182846492202 1102 3 0.7360241154164733 1102 4 0.7041257147401438 1102 5 0.7364665585668888 1102 6 0.8058872930764736 1102 7 0.8412913670574298 1102 8 0.8398204801741684 1102 9 1.0038666858487773 1102 10 1.0291354631230476 1102 11 1.0546545813438186 1102 12 1.041092807220572 1102 13 1.0349817097627394 1102 14 1.1982281586725918 1102 15 1.1165579499313338 1102 16 1.16215177169493 1102 17 1.1512098216464537 1102 18 1.172824074475514 1102 19 1.194099104145657 1102 20 1.2383781447427467 1102 21 1.1688924711404027 1102 22 1.2735201951879276 1102 23 1.2116449107268252 1102 24 1.2751788424739843 1102 25 1.3210345039703313 1102 26 1.3019085224405984 1102 27 1.3052829218581863 1102 28 1.2767512553335578 1102 29 1.3007719820250756 1102 30 1.2511973242140755 1102 31 1.2898030192841148 1102 32 1.3003146395283665 1102 33 1.3675699564993664 1102 34 1.4005148351927659 1102 35 1.4665124323882566 1102 36 1.449792100439737 1102 37 1.4037017405387715 1102 38 1.4512509835376264 1102 39 1.4308469903458203 1102 40 1.4610069452658934 1102 41 1.4804213672934594 1102 42 1.4360068201371519 1102 43 1.4130311959973696 1102 44 1.524203236378412 1102 45 1.5470574067512928 1102 46 1.528213625738367 1102 47 1.5914395622789286 1102 48 1.521797993799602 1102 49 1.4239640911233664 1102 50 1.3832486493912484 1102 51 1.4096740308393905 1102 52 1.4026895408374287 1102 53 1.4951492363414474 1102 54 1.5398127404306123 1102 55 1.5517715135736978 1102 56 1.5653169291991418 1102 57 1.4955250200318275 1102 58 1.4289416133439872 1102 59 1.3884700012153743 1102 60 1.4263837734953668 1102 61 1.4803665562442063 1102 62 1.4494178999429082 1102 63 1.5156357085979622 1102 64 1.47278402314857 1102 65 1.442200451007274 1102 66 1.4804743286920683 1102 67 1.487212738296435 1102 68 1.5413432515243954 1102 69 1.4704898656534908 1102 70 1.5090225119776264 1102 71 1.4468260674628937 1102 72 1.4153050033664982 1102 73 1.4157529055085831 1102 74 1.4514361288798554 1102 75 1.4411395060142738 1103 1 0.32895728838843397 1103 2 0.36550416557183496 1103 3 0.39036368654938514 1103 4 0.3907081562920638 1103 5 0.426459302762801 1103 6 0.4692429916226004 1103 7 0.4586802390501461 1103 8 0.5397662546833075 1103 9 0.6352631205165267 1103 10 0.6284950692920503 1103 11 0.6508050945680353 1103 12 0.6457438448902266 1103 13 0.530714684216484 1103 14 0.6714848290680422 1103 15 0.6381533201653937 1103 16 0.6989259453166383 1103 17 0.6728884854227601 1103 18 0.6615254698665254 1103 19 0.6518728899442578 1103 20 0.7823924301830374 1103 21 0.7937244580525515 1103 22 0.7663289286553194 1103 23 0.7927322451516403 1103 24 0.7649241470620751 1103 25 0.8289400923343422 1103 26 0.7286904350884882 1103 27 0.7769078956732756 1103 28 0.7880132446593322 1103 29 0.7412528926347193 1103 30 0.788235376958518 1103 31 0.8365354539737879 1103 32 0.8181044115098075 1103 33 0.7110918535596085 1103 34 0.7494929574365017 1103 35 0.8173595282109503 1103 36 0.7674141395523293 1103 37 0.8480808586683892 1103 38 0.8477687455617016 1103 39 0.9003355246559579 1103 40 0.8982383117607213 1103 41 0.8327741732322096 1103 42 0.8791885437804368 1103 43 0.8773499512172265 1103 44 1.015482995325307 1103 45 1.0280474242294844 1103 46 0.9636384389941952 1103 47 1.0015774125931642 1103 48 0.9337252604039534 1103 49 0.9732061921232571 1103 50 0.9937536986886215 1103 51 0.981868683662988 1103 52 0.9939013248809871 1103 53 0.9243748081563439 1103 54 1.0302853690119065 1103 55 1.1052559559391213 1103 56 1.010338981812069 1103 57 1.0027512080794523 1103 58 1.0094352321968216 1103 59 1.105111896522061 1103 60 1.0218024792223233 1103 61 1.079978882592492 1103 62 1.0916895718938804 1103 63 1.0227095073239951 1103 64 1.0564021959861591 1103 65 0.9826528913660795 1103 66 1.050305527254757 1103 67 1.0113663461347997 1103 68 1.0285430715864265 1103 69 1.0017653204364834 1103 70 0.96588006881165 1103 71 0.9209872598956395 1103 72 0.9200269551254863 1103 73 0.9319132226616986 1103 74 0.9754181679625589 1103 75 1.0250191667724309 1104 1 0.47761402004738684 1104 2 0.47450334751106027 1104 3 0.4452080819583557 1104 4 0.44344569341570406 1104 5 0.4998220687894346 1104 6 0.5080123597153374 1104 7 0.5450050943682498 1104 8 0.6299750921736873 1104 9 0.7713327074053424 1104 10 0.7081416145540871 1104 11 0.8154591263602171 1104 12 0.7633379418458333 1104 13 0.7400331323426457 1104 14 0.8577406474349729 1104 15 0.8523981504282432 1104 16 0.8667274763947646 1104 17 0.8577014305594446 1104 18 0.7386827961503144 1104 19 0.764635874044366 1104 20 0.814294613541982 1104 21 0.8419102723234744 1104 22 0.8568671677671418 1104 23 0.85864670284505 1104 24 0.8774081214759093 1104 25 0.9597666836070644 1104 26 0.8904867687614484 1104 27 0.9911754498465086 1104 28 0.9289752228983801 1104 29 0.9507840802984688 1104 30 0.9881300578140326 1104 31 0.9684522224701908 1104 32 0.9988657975200184 1104 33 0.9856226951353477 1104 34 0.939922796840257 1104 35 0.9232361215944351 1104 36 1.0591815900893167 1104 37 0.9619837463734839 1104 38 0.9994522260204661 1104 39 1.0077646650380032 1104 40 1.0072345462471333 1104 41 1.019080858929911 1104 42 0.9749007035255346 1104 43 1.0203082582611813 1104 44 1.0726456574494776 1104 45 1.0048684651633266 1104 46 0.9779332998915393 1104 47 0.9163158746465143 1104 48 0.9057146180075648 1104 49 0.9521399862480564 1104 50 0.9477754790337869 1104 51 0.956368890745317 1104 52 0.8947966469501054 1104 53 0.809946703376113 1104 54 0.780900198160424 1104 55 0.8188734066092209 1104 56 0.8978455470255042 1104 57 0.8617564275429856 1104 58 0.9069418867846366 1104 59 0.9365215890399838 1104 60 0.82656387055696 1104 61 0.8786044378070272 1104 62 0.8477471578958031 1104 63 0.8133902985648547 1104 64 0.7386921215163866 1104 65 0.7671423202790351 1104 66 0.7882012893500558 1104 67 0.8703392862360886 1104 68 0.9511973935600864 1104 69 0.8942429074348297 1104 70 0.8692482087103102 1104 71 0.8157513571821156 1104 72 0.8819239490659072 1104 73 0.9220611714817508 1104 74 0.9284338685682911 1104 75 0.9278306184062473 1105 1 0.3515697125170121 1105 2 0.42510330940095997 1105 3 0.3674947788106451 1105 4 0.34872448695876557 1105 5 0.3304013423383054 1105 6 0.45900304088804234 1105 7 0.45350024440408987 1105 8 0.5195801535581737 1105 9 0.6308978439211756 1105 10 0.6122358190964476 1105 11 0.6228323422176487 1105 12 0.7141102612084822 1105 13 0.6687629317201811 1105 14 0.756890792162622 1105 15 0.6833569824443586 1105 16 0.7309772971865485 1105 17 0.6976787913441456 1105 18 0.6871372954107926 1105 19 0.7442751891513879 1105 20 0.7193798622104026 1105 21 0.6660638197387883 1105 22 0.7513872073290528 1105 23 0.7958520527774482 1105 24 0.9018565231200881 1105 25 0.8149591849908262 1105 26 0.8482776531880276 1105 27 0.8553988657819787 1105 28 0.8405976697483979 1105 29 0.7991523327688199 1105 30 0.7529229929270471 1105 31 0.8224126787025554 1105 32 0.7873333177319779 1105 33 0.783013378122817 1105 34 0.7638057171629242 1105 35 0.7709183014922587 1105 36 0.8758286723980646 1105 37 0.8696306290578875 1105 38 0.8640515255205017 1105 39 0.8771130730338683 1105 40 0.921223857270931 1105 41 0.8045913074495132 1105 42 0.7932075261411526 1105 43 0.8526884156014631 1105 44 0.9130181836955416 1105 45 0.8580032025212105 1105 46 0.8278490947140114 1105 47 0.8613532134725403 1105 48 0.8371141207614698 1105 49 0.8343520961866275 1105 50 0.8715766441140431 1105 51 0.8248072385816627 1105 52 0.7688348659380111 1105 53 0.7776195372251422 1105 54 0.8276895581829322 1105 55 0.8414692036094138 1105 56 0.866870019155229 1105 57 0.858673274930176 1105 58 0.8159635006567143 1105 59 0.8378427392391572 1105 60 0.7553359993691089 1105 61 0.8412791317919854 1105 62 0.9119280175508138 1105 63 0.7854980140457215 1105 64 0.8620465817593868 1105 65 0.8662283583709893 1105 66 0.8581275190813784 1105 67 0.9367445055052883 1105 68 0.9154859142529688 1105 69 0.8031052301548058 1105 70 0.8941381884756581 1105 71 0.843189314843606 1105 72 0.8524731428557715 1105 73 0.8867983025169863 1105 74 0.9232973527389845 1105 75 0.9476008689347992 1106 1 0.06785749121904061 1106 2 0.09100948175719736 1106 3 0.13590718985162198 1106 4 0.11382134287912749 1106 5 0.15845068639293203 1106 6 0.18392178847548024 1106 7 0.18387054228429633 1106 8 0.19661624822308 1106 9 0.2679402452303492 1106 10 0.29079790420352936 1106 11 0.3093653732669921 1106 12 0.2628940031145035 1106 13 0.2313754047402483 1106 14 0.3127586353446503 1106 15 0.28098263961300063 1106 16 0.2575564319801984 1106 17 0.25998006409020036 1106 18 0.22195921426168042 1106 19 0.23843534529520127 1106 20 0.30907002112696347 1106 21 0.31938345243113275 1106 22 0.4005916459017982 1106 23 0.37267970197579814 1106 24 0.37248637203246204 1106 25 0.3970026464686498 1106 26 0.43269023820539587 1106 27 0.465519507537401 1106 28 0.40989953510154464 1106 29 0.28569754216571397 1106 30 0.26606832302006467 1106 31 0.32738633758921765 1106 32 0.38632447211971765 1106 33 0.38835206123025046 1106 34 0.4307933702757367 1106 35 0.46599074634990245 1106 36 0.5197946817601746 1106 37 0.5409655173052386 1106 38 0.43504052695036677 1106 39 0.43205797144796065 1106 40 0.48323458943971076 1106 41 0.3142703753709881 1106 42 0.405454594367086 1106 43 0.4670116746142554 1106 44 0.4699653329292275 1106 45 0.34382097312246174 1106 46 0.43704657423100457 1106 47 0.4314123110787307 1106 48 0.3717714144499311 1106 49 0.39845098001932655 1106 50 0.30545354494206833 1106 51 0.35445640431048986 1106 52 0.31494067620587884 1106 53 0.3344892669636863 1106 54 0.35123584949553077 1106 55 0.36264724126442616 1106 56 0.33456031942760944 1106 57 0.29515090537169897 1106 58 0.33444642650471934 1106 59 0.3386479237356852 1106 60 0.285156314375115 1106 61 0.368478927369722 1106 62 0.3314246820971434 1106 63 0.33590060629398266 1106 64 0.31272046844638623 1106 65 0.2798365744061755 1106 66 0.2801266165788405 1106 67 0.26522672457418395 1106 68 0.2942201272544409 1106 69 0.29593272712381236 1106 70 0.2936988980818711 1106 71 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-1.2780682543734052 2216 67 -1.2360358865491392 2216 68 -1.3721273154380853 2216 69 -1.4494598043822577 2216 70 -1.323851870224285 2216 71 -1.2179016159591214 2216 72 -1.3057243621167416 2216 73 -1.3015070118243877 2216 74 -1.306423104172719 2216 75 -1.2501344626097932 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 60925.0 3 24303.0 4 14283.0 5 9251.0 6 6909.0 7 6215.0 8 5876.0 9 6148.0 10 6622.0 11 7109.0 12 7736.0 13 7941.0 14 8334.0 15 8759.0 16 9401.0 17 10047.0 18 10522.0 19 11437.0 20 12947.0 21 14837.0 22 18592.0 23 22370.0 24 24156.0 25 25861.0 26 27533.0 27 29319.0 28 34090.0 29 40411.0 30 46961.0 31 53550.0 32 65768.0 33 81532.0 34 106805.0 35 145330.0 36 206194.0 37 326155.0 38 564182.0 39 869516.0 40 224878.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.74668562209115 18.04210040700583 14.350443947390154 25.86077002351287 2 15.337308463891159 19.294077021738413 38.36847411147737 27.00014040289306 3 18.34889739418573 23.35089724558017 29.168457047460507 29.1317483127736 4 12.670242010095311 16.057351142925715 35.74251068138599 35.52989616559299 5 14.225660433010749 36.99931292750166 34.00559468798965 14.769431951497936 6 33.646480174872046 35.85626778278685 16.45316512645414 14.044086915886963 7 29.02689286434907 31.532242404538458 20.555801887060976 18.885062844051497 8 26.786013028860644 34.95300685598014 18.90778692477713 19.353193190382083 9 26.24806688537077 15.791897699368212 18.781507514671745 39.17852790058927 10 14.844596979353392 29.01442517517549 32.875907819550584 23.265070025920533 11 35.41114075130946 22.79655903859508 22.49188267540947 19.30041753468599 12 23.605591150225685 24.533050081136434 29.563793937824833 22.297564830813045 13 28.33032446706676 21.572700907090113 25.6231675846214 24.473807041221733 14 22.22016966689452 20.880466101574285 26.66631028120395 30.23305395032725 15 23.867699943963704 28.797186547746595 23.40103648178061 23.93407702650909 16 23.67568121468051 28.03840913489942 24.896864242855138 23.389045407564936 17 22.576115789353846 27.830220899917652 26.319384622390547 23.274278688337958 18 22.61011484249792 27.082919597602206 27.405570221652724 22.901395338247145 19 23.357825342335804 26.786559035059536 27.62415285644537 22.23146276615929 20 23.541578587715883 26.13042778464139 27.707548264629906 22.620445363012816 21 23.663096107806208 25.96318118135213 27.609085475194263 22.7646372356474 22 23.510752710273692 25.71008807144377 27.46535058607307 23.313808632209465 23 23.33350514534822 25.810981696960923 27.45759431768126 23.397918840009602 24 23.112490485091914 25.840482229930778 27.565325160816574 23.481702124160737 25 23.27865327360953 25.762798691473233 27.65886599617055 23.299682038746685 26 23.196479491532713 26.020362981274054 27.662583780966383 23.120573746226853 27 23.129685649361225 26.21262747417309 27.459403557654145 23.198283318811544 28 23.14616315472188 26.084026054453226 27.489889887863626 23.27992090296127 29 23.255057706329588 26.167436137115995 27.48864774069874 23.08885841585568 30 23.31072362045539 26.06016633731507 27.47921416119083 23.14989588103871 31 23.33913620947276 25.775079255903677 27.544972290080562 23.340812244543006 32 23.163111848088267 26.122509381913112 27.453431939864686 23.260946830133932 33 23.19688312575358 25.96788071334452 27.415673986742345 23.419562174159555 34 23.426952773088143 25.853359444196567 27.458968831711733 23.260718951003554 35 23.297389608455497 26.06995635593046 27.364954915673007 23.26769911994104 36 23.35999676206455 26.059625442963164 27.312807647345828 23.267570147626458 37 23.382289515189612 25.8396056860337 27.4638615094879 23.31424328928879 38 23.365969674319544 26.017443147253704 27.35726580690365 23.2593213715231 39 23.386653841666263 26.271330337671255 27.215308547818502 23.12670727284398 40 23.455202826665623 25.844233623635315 27.455482356423204 23.245081193275855 41 23.227354639112015 26.03308594396 27.31833861345376 23.421220803474224 42 23.41591419962771 26.000593815778615 27.19499196388285 23.38850002071083 43 23.254672667429148 25.936770649175788 27.287100310609976 23.521456372785092 44 23.397866625855098 26.11214158915947 27.093484842064925 23.39650694292051 45 23.377981347370444 26.14131630802387 26.974822491418628 23.505879853187057 46 23.438770157714163 25.94162606114242 26.909171046561735 23.71043273458168 47 23.26929102786936 26.230131766123904 26.93966355419144 23.560913651815298 48 23.262063535728174 26.27945728713128 26.87301391563351 23.58546526150704 49 23.49724892704677 26.15255127948132 26.74162426180122 23.608575531670695 50 23.558529718176484 26.147632078588718 26.775747400637727 23.51809080259707 51 23.284131301256945 26.366108901706177 26.61336270868759 23.736397088349285 52 23.504821961623502 26.334193037189223 26.539902242280927 23.621082758906343 53 23.322809974057837 26.43792456379701 26.640055267397138 23.599210194748014 54 23.269952864610012 26.41734992114592 26.713479558012448 23.599217656231623 55 23.39162038639874 26.162460734887972 26.678997532246 23.76692134646729 56 23.217708028001518 26.432304635857918 26.737154245807883 23.612833090332686 57 23.48981808351369 26.2040999584218 26.517691287390736 23.788390670673774 58 23.279526443724404 26.162011286230648 26.74807575079234 23.81038651925261 59 23.382480024967013 26.291567024697926 26.717074989257167 23.608877961077894 60 23.352453739830096 26.394982363527596 26.777559122337298 23.475004774305006 61 23.497072351291287 26.223600336033808 26.767141660635573 23.51218565203933 62 23.20188733245102 26.490646351278485 26.674667590093403 23.632798726177093 63 23.129339247109698 26.287446336969523 26.87785429457601 23.70536012134477 64 23.284376191701867 26.236128827438094 26.78828797777956 23.691207003080482 65 23.301579069697578 26.200951521121464 26.7882446506583 23.70922475852266 66 23.23418416813553 26.278386721588614 26.6337603002366 23.853668810039256 67 23.294008717042693 26.26525348076138 26.638082086170524 23.8026557160254 68 23.267197268839734 26.255067878983745 26.777279585013403 23.700455267163118 69 23.220594349928778 26.32125630214237 26.619964807603836 23.838184540325017 70 23.219278038044433 26.023295929773855 26.771156305980824 23.986269726200884 71 23.172173092586878 26.210203730591967 26.67703363806074 23.940589538760413 72 23.13646732781972 26.312297737697932 26.498171931415648 24.0530630030667 73 23.117782668951275 26.151666359431267 26.674904580484554 24.0556463911329 74 23.179859263784902 26.314547844114973 26.51244628787045 23.993146604229675 75 23.235038850376647 26.315751199500436 26.498913137988 23.950296812134912 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 463.0 1 369.0 2 333.0 3 399.5 4 408.0 5 339.0 6 336.0 7 656.5 8 911.0 9 1650.0 10 2823.5 11 3302.5 12 3347.0 13 3120.0 14 2995.5 15 3338.5 16 3579.0 17 3951.0 18 4623.0 19 5554.5 20 6186.0 21 7039.0 22 9185.0 23 11979.5 24 13481.0 25 15616.0 26 20580.5 27 26371.0 28 29332.0 29 33193.0 30 40710.5 31 48766.0 32 53165.0 33 57866.5 34 67914.5 35 78657.0 36 84053.0 37 88405.0 38 96824.0 39 105811.0 40 110731.0 41 115946.0 42 125584.0 43 134656.0 44 139305.0 45 143692.0 46 150923.5 47 156733.0 48 159698.0 49 162401.5 50 165416.5 51 165398.0 52 165068.0 53 160918.5 54 153951.5 55 144922.0 56 138710.0 57 132135.5 58 117535.5 59 100230.5 60 90951.0 61 81595.0 62 63108.5 63 46575.0 64 39172.0 65 32381.0 66 21598.5 67 14972.5 68 12338.0 69 10271.5 70 6808.0 71 4376.5 72 3342.0 73 2822.0 74 2087.0 75 1378.0 76 884.0 77 680.5 78 379.0 79 197.5 80 114.0 81 90.0 82 49.0 83 23.0 84 14.0 85 10.5 86 5.5 87 3.0 88 2.0 89 1.5 90 1.0 91 2.5 92 4.0 93 2.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07613494983092539 2 0.015081549447405073 3 0.002213225285782715 4 0.14250009090032423 5 0.09554809733764806 6 0.06620705354898579 7 0.026906495974301295 8 0.02364989305379244 9 0.04603508594428048 10 0.02633738090081431 11 0.013690379267770222 12 0.10471717352160503 13 0.08536726102304758 14 0.04533950085446305 15 0.01799035982300521 16 0.030131481390727535 17 0.020519760149614028 18 0.01773741979034433 19 0.013058029186118017 20 0.012520531616713645 21 0.026400615908979526 22 0.015366106984148566 23 0.013721996771852834 24 0.020930787702687963 25 0.014860226918826802 26 0.0312697115377015 27 0.02813957863352309 28 0.03537998706844083 29 0.04869095628721973 30 0.03278735173366679 31 0.018338152367913925 32 0.010655098875839642 33 0.012836706657539748 34 0.012394061600383205 35 0.005564680718539398 36 0.009358781208452623 37 0.007872758516569944 38 0.0023080777980305457 39 0.007335260947165569 40 0.011097743932996186 41 0.009927896281939607 42 0.006576440849182925 43 0.010054366298270048 44 0.009580103737030896 45 0.004458068075648041 46 0.0018338152367913922 47 0.0049323306368871936 48 0.005533063214456787 49 0.0012330826592217984 50 3.1617504082610213E-4 51 0.0019919027572044435 52 0.002782340359269699 53 0.0 54 3.161750408261021E-5 55 3.1617504082610213E-4 56 0.008283786069643877 57 0.0028455753674349192 58 4.110275530739328E-4 59 0.007335260947165569 60 0.0012963176673870189 61 8.852901143130861E-4 62 0.021183727735348844 63 0.007777906004322113 64 0.009833043769691775 65 0.015808752041305107 66 0.015777134537222497 67 0.015619047016809447 68 0.015366106984148566 69 0.006007325775695941 70 0.014227876837174597 71 0.006228648304274212 72 0.014259494341257206 73 0.006070560783861161 74 0.018243299855666095 75 9.485251224783063E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3162805.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.422364513124 #Duplication Level Percentage of deduplicated Percentage of total 1 76.78323828361606 46.394248120707076 2 12.72024742879701 15.371748536798027 3 4.529892567915499 8.211204598316447 4 2.050430352815235 4.955674007463024 5 1.1245527606515549 3.3974068409164073 6 0.6735571334386667 2.4418748782227615 7 0.45374236313785005 1.9191330522393018 8 0.31854855220497125 1.539796538916535 9 0.22108838341684342 1.2022814603186849 >10 1.0610898959149817 10.98211024088123 >50 0.049153392184601345 2.004358260442487 >100 0.013827950409732148 1.2621890905507556 >500 4.7320162311860736E-4 0.18715026879074687 >1k 1.5773387391521593E-4 0.1308241054364347 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.161750408261021E-5 0.0 0.0 0.0 3.161750408261021E-5 2 3.161750408261021E-5 0.0 0.0 0.0 3.161750408261021E-5 3 3.161750408261021E-5 0.0 0.0 0.0 3.161750408261021E-5 4 3.161750408261021E-5 0.0 0.0 0.0 3.161750408261021E-5 5 3.161750408261021E-5 0.0 0.0 0.0 3.161750408261021E-5 6 3.161750408261021E-5 0.0 0.0 0.0 3.161750408261021E-5 7 6.323500816522043E-5 0.0 0.0 0.0 6.323500816522043E-5 8 6.323500816522043E-5 0.0 0.0 0.0 6.323500816522043E-5 9 6.323500816522043E-5 0.0 0.0 0.0 6.323500816522043E-5 10 6.323500816522043E-5 0.0 0.0 0.0 6.323500816522043E-5 11 6.323500816522043E-5 0.0 0.0 0.0 6.323500816522043E-5 12 6.323500816522043E-5 0.0 0.0 0.0 9.485251224783064E-5 13 6.323500816522043E-5 0.0 0.0 0.0 1.2647001633044085E-4 14 6.323500816522043E-5 0.0 0.0 0.0 1.5808752041305106E-4 15 6.323500816522043E-5 0.0 0.0 0.0 2.213225285782715E-4 16 6.323500816522043E-5 0.0 0.0 0.0 2.213225285782715E-4 17 6.323500816522043E-5 0.0 0.0 0.0 2.213225285782715E-4 18 6.323500816522043E-5 0.0 0.0 3.161750408261021E-5 2.213225285782715E-4 19 6.323500816522043E-5 0.0 0.0 3.161750408261021E-5 2.213225285782715E-4 20 6.323500816522043E-5 0.0 0.0 3.161750408261021E-5 2.213225285782715E-4 21 6.323500816522043E-5 0.0 0.0 9.485251224783064E-5 2.213225285782715E-4 22 6.323500816522043E-5 0.0 0.0 1.8970502449566128E-4 2.213225285782715E-4 23 6.323500816522043E-5 0.0 0.0 2.529400326608817E-4 2.529400326608817E-4 24 9.485251224783064E-5 0.0 0.0 4.42645057156543E-4 2.529400326608817E-4 25 9.485251224783064E-5 0.0 0.0 5.058800653217634E-4 2.529400326608817E-4 26 9.485251224783064E-5 0.0 0.0 7.27202593900035E-4 2.8455753674349194E-4 27 9.485251224783064E-5 0.0 0.0 0.0010117601306435268 2.8455753674349194E-4 28 1.2647001633044085E-4 3.161750408261021E-5 0.0 0.0019602852531218334 2.8455753674349194E-4 29 1.2647001633044085E-4 3.161750408261021E-5 0.0 0.0032566029205088522 2.8455753674349194E-4 30 1.2647001633044085E-4 3.161750408261021E-5 0.0 0.005216888173630686 2.8455753674349194E-4 31 1.2647001633044085E-4 3.161750408261021E-5 0.0 0.010749951388087473 2.8455753674349194E-4 32 1.2647001633044085E-4 3.161750408261021E-5 0.0 0.017199922220939957 2.8455753674349194E-4 33 1.2647001633044085E-4 3.161750408261021E-5 0.0 0.0250726807375099 2.8455753674349194E-4 34 1.2647001633044085E-4 3.161750408261021E-5 0.0 0.03610718966234087 3.1617504082610213E-4 35 1.5808752041305106E-4 3.161750408261021E-5 0.0 0.047046846074924 3.1617504082610213E-4 36 1.5808752041305106E-4 3.161750408261021E-5 0.0 0.06402544576728568 3.1617504082610213E-4 37 1.5808752041305106E-4 3.161750408261021E-5 0.0 0.08340697576992574 3.1617504082610213E-4 38 1.8970502449566128E-4 3.161750408261021E-5 0.0 0.10579216866041379 3.4779254490871237E-4 39 1.8970502449566128E-4 3.161750408261021E-5 0.0 0.12827221406314965 3.4779254490871237E-4 40 1.8970502449566128E-4 3.161750408261021E-5 0.0 0.1511000520107942 3.4779254490871237E-4 41 2.213225285782715E-4 3.161750408261021E-5 0.0 0.17256833728288656 3.4779254490871237E-4 42 2.213225285782715E-4 3.161750408261021E-5 0.0 0.19416309257130931 3.4779254490871237E-4 43 2.213225285782715E-4 3.161750408261021E-5 0.0 0.2172438705516148 3.7941004899132256E-4 44 2.529400326608817E-4 3.161750408261021E-5 0.0 0.24724888192601188 3.7941004899132256E-4 45 2.529400326608817E-4 3.161750408261021E-5 0.0 0.27927741356169605 4.110275530739328E-4 46 2.8455753674349194E-4 3.161750408261021E-5 0.0 0.31468901813421946 4.42645057156543E-4 47 3.1617504082610213E-4 3.161750408261021E-5 0.0 0.35231384799252563 4.742625612391532E-4 48 3.4779254490871237E-4 3.161750408261021E-5 0.0 0.39050779292431875 4.742625612391532E-4 49 3.4779254490871237E-4 3.161750408261021E-5 0.0 0.4281958577907901 4.742625612391532E-4 50 3.4779254490871237E-4 3.161750408261021E-5 0.0 0.4667692127715746 5.058800653217634E-4 51 3.4779254490871237E-4 3.161750408261021E-5 0.0 0.5049315401992851 5.058800653217634E-4 52 3.4779254490871237E-4 3.161750408261021E-5 0.0 0.5425563700575913 5.058800653217634E-4 53 3.4779254490871237E-4 3.161750408261021E-5 0.0 0.5825841302261758 6.007325775695941E-4 54 3.4779254490871237E-4 3.161750408261021E-5 0.0 0.6272280459908215 6.007325775695941E-4 55 3.4779254490871237E-4 3.161750408261021E-5 0.0 0.6785432551168978 6.323500816522043E-4 56 3.4779254490871237E-4 3.161750408261021E-5 0.0 0.7321349245369221 6.323500816522043E-4 57 3.4779254490871237E-4 3.161750408261021E-5 0.0 0.7892361369101162 6.639675857348145E-4 58 3.4779254490871237E-4 3.161750408261021E-5 0.0 0.8484873395609277 6.639675857348145E-4 59 3.4779254490871237E-4 3.161750408261021E-5 0.0 0.907833394723987 6.639675857348145E-4 60 3.4779254490871237E-4 3.161750408261021E-5 0.0 0.9698985552381509 6.639675857348145E-4 61 3.4779254490871237E-4 3.161750408261021E-5 0.0 1.035346788689154 6.639675857348145E-4 62 3.4779254490871237E-4 3.161750408261021E-5 0.0 1.100415612091166 6.955850898174247E-4 63 3.4779254490871237E-4 3.161750408261021E-5 0.0 1.167413103242217 6.955850898174247E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3430 0.0 38.61746 1 GTATCAA 6520 0.0 33.629524 1 TATCAAC 8790 0.0 24.852283 2 TCAACGC 8925 0.0 24.44306 4 ATCAACG 9015 0.0 24.160688 3 CAACGCA 9010 0.0 24.10897 5 AACGCAG 9520 0.0 22.740545 6 TATAGCG 110 3.244141E-5 21.976482 5 GTACATG 8390 0.0 19.744793 1 GTGGTAT 1720 0.0 19.503939 1 ACGCAGA 11145 0.0 19.175797 7 TACATGG 8625 0.0 18.43473 2 CGCAGAG 11595 0.0 18.342836 8 GCGATAC 360 0.0 18.206923 14 AGAGTAC 11055 0.0 17.99012 11 TGGTATC 1800 0.0 17.666618 2 GAGTACT 7175 0.0 17.468475 12 TACGCTA 435 0.0 17.443336 31 ACATGGG 9035 0.0 17.410757 3 CAGAGTA 11880 0.0 17.00238 10 >>END_MODULE