FastQCFastQC Report
Mon 6 Feb 2017
SRR2077512_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077512_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369559
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33500.040.492721
GTATCAA70050.035.9210131
TCAACGC96000.025.8381084
TATCAAC96000.025.8373412
ATCAACG96250.025.698933
CAACGCA98900.025.2203755
AACGCAG103150.024.1977736
GTGGTAT16150.022.6549221
TGGTATC16150.020.933592
ACGCAGA118350.020.9167657
GTACATG122950.020.1850381
CGCAGAG122600.020.052858
GAGTACT61850.019.68087212
TACATGG124050.019.6335182
AGAGTAC119050.019.17482811
ACATGGG126150.018.8967633
ACTCGAC4600.018.75460846
AGTACTT67100.018.55462313
GCAGAGT128450.018.2811269
CAGAGTA126600.018.27617310
Produced by FastQC (version 0.11.5)