FastQCFastQC Report
Mon 6 Feb 2017
SRR2077512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369559
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42100.044.136391
GTATCAA86800.034.761731
GTGGTAT16150.026.7819441
CGTGCCG4100.025.26230810
TATCAAC119750.024.9778082
ATCAACG122550.024.3825323
TCAACGC123350.024.2243964
CAACGCA124950.023.9640645
TGGTATC18150.023.4337712
AACGCAG131550.022.8340726
GTATACG1507.134986E-720.7613641
GTACATG122000.019.8254011
ACGCAGA153500.019.4327647
TACATGG123150.018.9251442
CGCAGAG156650.018.8659638
CGAATCG3700.018.64174837
ACATGGG124900.018.4135533
GAGTACT96050.018.1881612
AGAGTAC154200.018.18056111
GTGCCGA5350.018.0692411
Produced by FastQC (version 0.11.5)