##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077512_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3369559 Sequences flagged as poor quality 0 Sequence length 75 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89695417115415 31.0 31.0 34.0 27.0 34.0 2 30.964625341179662 33.0 31.0 34.0 27.0 34.0 3 31.04390010680923 33.0 31.0 34.0 27.0 34.0 4 34.44011812821797 37.0 35.0 37.0 31.0 37.0 5 34.1690734603549 37.0 35.0 37.0 30.0 37.0 6 34.15297491452146 37.0 35.0 37.0 30.0 37.0 7 33.98870712754992 37.0 35.0 37.0 30.0 37.0 8 33.92853278426049 37.0 35.0 37.0 29.0 37.0 9 35.39109628292604 39.0 35.0 39.0 29.0 39.0 10 35.28018770408828 39.0 35.0 39.0 28.0 39.0 11 35.288936920232 39.0 35.0 39.0 28.0 39.0 12 35.192680407139335 39.0 35.0 39.0 27.0 39.0 13 35.122242702976855 39.0 35.0 39.0 27.0 39.0 14 36.360726730115125 40.0 36.0 41.0 27.0 41.0 15 36.40192351580726 40.0 36.0 41.0 28.0 41.0 16 36.38352704315312 40.0 36.0 41.0 27.0 41.0 17 36.38598611865826 40.0 36.0 41.0 27.0 41.0 18 36.39209314928155 40.0 36.0 41.0 28.0 41.0 19 36.43367870988459 40.0 37.0 41.0 27.0 41.0 20 36.39993542181632 40.0 37.0 41.0 27.0 41.0 21 36.37660655296435 40.0 37.0 41.0 27.0 41.0 22 36.36504598969776 40.0 37.0 41.0 27.0 41.0 23 36.34325649142811 40.0 37.0 41.0 27.0 41.0 24 36.33169414751307 40.0 36.0 41.0 27.0 41.0 25 36.304204496790234 40.0 36.0 41.0 27.0 41.0 26 36.239471990251545 40.0 36.0 41.0 27.0 41.0 27 36.187458655568875 40.0 36.0 41.0 27.0 41.0 28 36.16604279669832 40.0 36.0 41.0 27.0 41.0 29 36.11173806423927 40.0 36.0 41.0 27.0 41.0 30 36.028114658327695 40.0 36.0 41.0 27.0 41.0 31 35.94097476850828 40.0 36.0 41.0 27.0 41.0 32 35.86790764013926 40.0 36.0 41.0 27.0 41.0 33 35.810598063426106 40.0 35.0 41.0 27.0 41.0 34 35.75000140968002 40.0 35.0 41.0 27.0 41.0 35 35.671247780495904 40.0 35.0 41.0 26.0 41.0 36 35.616036104427906 40.0 35.0 41.0 26.0 41.0 37 35.55951772917465 40.0 35.0 41.0 26.0 41.0 38 35.49966212195721 40.0 35.0 41.0 25.0 41.0 39 35.420735769873744 40.0 35.0 41.0 25.0 41.0 40 35.356698309778814 40.0 35.0 41.0 25.0 41.0 41 35.30300493328652 40.0 35.0 41.0 25.0 41.0 42 35.24299767417635 40.0 35.0 41.0 25.0 41.0 43 35.148829268162395 40.0 35.0 41.0 24.0 41.0 44 35.02472311658588 39.0 35.0 41.0 24.0 41.0 45 34.9322964815277 39.0 35.0 41.0 23.0 41.0 46 34.7979251884297 39.0 35.0 41.0 23.0 41.0 47 34.64955799853927 39.0 34.0 41.0 22.0 41.0 48 34.54583582005835 39.0 34.0 41.0 21.0 41.0 49 34.450583592689725 39.0 34.0 41.0 20.0 41.0 50 34.45475921329764 39.0 34.0 41.0 20.0 41.0 51 34.37943036462635 39.0 34.0 41.0 19.0 41.0 52 34.317763244388956 39.0 34.0 41.0 19.0 41.0 53 34.224104697380284 39.0 34.0 41.0 18.0 41.0 54 34.193504847370235 39.0 34.0 41.0 18.0 41.0 55 34.11275273707924 39.0 34.0 41.0 18.0 41.0 56 33.992623367034085 39.0 34.0 41.0 16.0 41.0 57 33.91815902318375 39.0 34.0 41.0 15.0 41.0 58 33.81663386811152 38.0 34.0 41.0 14.0 41.0 59 33.725156615450274 38.0 34.0 41.0 13.0 41.0 60 33.694396210305264 38.0 34.0 41.0 13.0 41.0 61 33.57948592085789 38.0 34.0 41.0 13.0 41.0 62 33.43825319574461 38.0 34.0 40.0 9.0 41.0 63 33.301741266438725 37.0 33.0 40.0 8.0 41.0 64 33.150484974443245 37.0 33.0 40.0 7.0 41.0 65 33.025380472637515 37.0 33.0 40.0 2.0 41.0 66 32.82816416035452 37.0 33.0 40.0 2.0 41.0 67 32.63932906353621 36.0 33.0 40.0 2.0 41.0 68 32.428397900140645 36.0 33.0 39.0 2.0 41.0 69 32.26553830931585 36.0 33.0 39.0 2.0 41.0 70 32.075121403127234 36.0 33.0 39.0 2.0 41.0 71 31.891106224879874 35.0 32.0 39.0 2.0 41.0 72 31.69585337428429 35.0 32.0 39.0 2.0 41.0 73 31.49527786870626 35.0 32.0 38.0 2.0 40.0 74 31.23002891476303 35.0 32.0 37.0 2.0 40.0 75 31.00940568187113 35.0 32.0 37.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.495992336475787 1101 2 0.5240619754499569 1101 3 0.5604358279667458 1101 4 0.5356051240354276 1101 5 0.5295005811756468 1101 6 0.5510515058055532 1101 7 0.5532053212902 1101 8 0.6330188330223905 1101 9 0.8039159189022982 1101 10 0.6784057167017252 1101 11 0.8110027404701654 1101 12 0.8877182650300384 1101 13 0.8119207034562947 1101 14 0.9274490028362479 1101 15 0.9231734951616417 1101 16 0.8635981732558093 1101 17 0.8922291884511182 1101 18 0.878769002437167 1101 19 0.8099998188862614 1101 20 0.859294828930075 1101 21 0.7784128446699938 1101 22 0.8189787509369708 1101 23 0.8344019099953357 1101 24 0.87247325991774 1101 25 0.9636766430862025 1101 26 0.8831602411397839 1101 27 0.9919284778903972 1101 28 0.9982610500757616 1101 29 0.9591139267192901 1101 30 0.9034819862539436 1101 31 0.935407291552032 1101 32 0.973821196607588 1101 33 0.9229865992825879 1101 34 0.895939518563452 1101 35 0.9903838905550444 1101 36 1.0122699646088336 1101 37 0.9931278127909167 1101 38 1.0697196682653924 1101 39 1.0635141491980917 1101 40 1.02142303295021 1101 41 1.0059665038829237 1101 42 1.0141548707553127 1101 43 1.0306788105368767 1101 44 1.0586150622255204 1101 45 1.1356805402475132 1101 46 1.1219815081796582 1101 47 1.0764639603411723 1101 48 1.1328095592667466 1101 49 1.167572624925164 1101 50 1.0355697473927066 1101 51 1.0906160142521344 1101 52 1.0896776759819602 1101 53 1.131883538592028 1101 54 1.1165085704204714 1101 55 1.031425654713928 1101 56 1.121065955337869 1101 57 1.110497290075351 1101 58 1.0573250578557705 1101 59 1.132081537284023 1101 60 1.0890000692093906 1101 61 1.0028293938672377 1101 62 1.089696376066101 1101 63 1.0886129088278054 1101 64 1.124204863139795 1101 65 0.9975369202596696 1101 66 1.1076141444836907 1101 67 1.1771787098855455 1101 68 1.2206149834776596 1101 69 1.1298066618375415 1101 70 1.103733061316369 1101 71 1.1001459056737204 1101 72 1.149109853825916 1101 73 1.1227442516377053 1101 74 1.1026476887423406 1101 75 1.1076554586486296 1102 1 0.7239046357373233 1102 2 0.7101617403363996 1102 3 0.7312185345951256 1102 4 0.7322599832674825 1102 5 0.7747532816691347 1102 6 0.847381884864916 1102 7 0.9152522515297221 1102 8 0.9978497575976562 1102 9 1.140051361425762 1102 10 1.1634552685919815 1102 11 1.094649271009743 1102 12 1.1131797107541388 1102 13 1.2139383186857415 1102 14 1.3150439160471166 1102 15 1.1685228082118613 1102 16 1.2220485285484841 1102 17 1.2376399539883138 1102 18 1.3545737677825187 1102 19 1.374371557781167 1102 20 1.3148568483257321 1102 21 1.321210137034086 1102 22 1.3717706122951867 1102 23 1.3388443623808755 1102 24 1.396772180548453 1102 25 1.4127029955412311 1102 26 1.414618669791757 1102 27 1.3427548947998034 1102 28 1.3179982363178553 1102 29 1.410003693862329 1102 30 1.5356010377670657 1102 31 1.399954106201534 1102 32 1.3990944893774468 1102 33 1.3667813817904744 1102 34 1.390305281281634 1102 35 1.4236622237400098 1102 36 1.4816063467584186 1102 37 1.4463883830672373 1102 38 1.4981018150930154 1102 39 1.4824091154768624 1102 40 1.4223763573537838 1102 41 1.417114523291005 1102 42 1.4428882732356314 1102 43 1.555763398280746 1102 44 1.5929018234758843 1102 45 1.5835563406652966 1102 46 1.4987578853053947 1102 47 1.5486404066138917 1102 48 1.5359570238427196 1102 49 1.5845663941053445 1102 50 1.6075542107134027 1102 51 1.5540233182994427 1102 52 1.4327295424330444 1102 53 1.5034147164992149 1102 54 1.526949937985016 1102 55 1.5893742112878257 1102 56 1.5476040099888309 1102 57 1.5350233882323252 1102 58 1.5354355853810304 1102 59 1.5686296879471655 1102 60 1.5510584204333 1102 61 1.5129305817186207 1102 62 1.5226926350898822 1102 63 1.4899366388730044 1102 64 1.5298616663545133 1102 65 1.4796604918602654 1102 66 1.6074709508728233 1102 67 1.5474208002500305 1102 68 1.6300307052390153 1102 69 1.642658029629814 1102 70 1.6373671702415038 1102 71 1.604901141211684 1102 72 1.6211922753782453 1102 73 1.7268587734020997 1102 74 1.6886309664889474 1102 75 1.7236007055523714 1103 1 0.6159204257576185 1103 2 0.7006833280222295 1103 3 0.6879442407442831 1103 4 0.7117869717976859 1103 5 0.6922267748891997 1103 6 0.74481186526166 1103 7 0.7765630943403394 1103 8 0.8640966803328709 1103 9 0.9751130426080294 1103 10 0.9829042291081933 1103 11 0.9366209464232966 1103 12 0.9368873404295641 1103 13 0.9815728375000461 1103 14 1.041073685560832 1103 15 1.051134438226363 1103 16 1.1176134656702033 1103 17 1.0989566205093837 1103 18 1.0939644232508385 1103 19 1.1501862059012566 1103 20 1.1847562081381824 1103 21 1.2102606069266315 1103 22 1.218384755564287 1103 23 1.1746964504884332 1103 24 1.1579744920438415 1103 25 1.2102870777875552 1103 26 1.284208968525455 1103 27 1.2851163382129158 1103 28 1.2462403363737309 1103 29 1.22650310679996 1103 30 1.1268995084283446 1103 31 1.1810584628869734 1103 32 1.1927246766453266 1103 33 1.2853926015569215 1103 34 1.259490670368585 1103 35 1.2778107924175686 1103 36 1.322479686556072 1103 37 1.3656761238655335 1103 38 1.2946718437553812 1103 39 1.3277775509014589 1103 40 1.2781501456742035 1103 41 1.2717327095093225 1103 42 1.2437730242703893 1103 43 1.3357474186663225 1103 44 1.3818624464576033 1103 45 1.3778733213431948 1103 46 1.3534275142994474 1103 47 1.456345110845433 1103 48 1.3940517225514384 1103 49 1.331634560312743 1103 50 1.336023386946323 1103 51 1.3580385563614001 1103 52 1.426031547439493 1103 53 1.3411400981382897 1103 54 1.4226321581646246 1103 55 1.4151282281112287 1103 56 1.4278139256323854 1103 57 1.3701071509834222 1103 58 1.3459271938995627 1103 59 1.3269651970834317 1103 60 1.3151526894816072 1103 61 1.3358107591994326 1103 62 1.31779945649901 1103 63 1.3215996188074115 1103 64 1.3606239124043356 1103 65 1.2663703316338157 1103 66 1.3346962796131905 1103 67 1.275894263380927 1103 68 1.3510190018457635 1103 69 1.2781999566244266 1103 70 1.34110288637833 1103 71 1.3136734771634195 1103 72 1.3710663376395367 1103 73 1.3615943591525124 1103 74 1.3618126665788601 1103 75 1.4297538277244328 1104 1 0.5044295201828888 1104 2 0.5682345021671367 1104 3 0.546754648195698 1104 4 0.583749695273255 1104 5 0.6109985577381281 1104 6 0.604275512911606 1104 7 0.6980526954753117 1104 8 0.7174300105267619 1104 9 0.8653397278664912 1104 10 0.8435166784865942 1104 11 0.897806485947271 1104 12 0.8612799871566708 1104 13 0.8689120174234191 1104 14 0.9661688617865636 1104 15 0.9282174409367983 1104 16 1.021389135937973 1104 17 0.9846013630128212 1104 18 0.9922498305884488 1104 19 0.9585384975633175 1104 20 1.037783128415569 1104 21 1.0225293573063112 1104 22 0.9954837066597264 1104 23 1.011553890292312 1104 24 1.069112398325423 1104 25 1.0165054880946087 1104 26 1.0546244318032763 1104 27 1.0964163340845445 1104 28 1.0556606675193407 1104 29 0.9966426912723207 1104 30 1.0454472133286004 1104 31 1.038060628005816 1104 32 1.0694967957289023 1104 33 1.0210602070748749 1104 34 1.0394572698040179 1104 35 1.0079765975418056 1104 36 1.1099756974976671 1104 37 1.0442923063590115 1104 38 1.0325453024248077 1104 39 1.0751410050202637 1104 40 1.0601752012777084 1104 41 1.0237315554490252 1104 42 1.060764913982915 1104 43 0.9343166790018884 1104 44 1.0208416336582076 1104 45 1.090408155457233 1104 46 1.1802888014942283 1104 47 1.17900881764254 1104 48 1.1847573459739706 1104 49 1.178932384315722 1104 50 1.2396589594423304 1104 51 1.234368719953217 1104 52 1.2078281298701228 1104 53 1.1082282197152153 1104 54 1.113236785613644 1104 55 1.1580883739447714 1104 56 1.2087723545029831 1104 57 1.142899410240112 1104 58 1.179485390043041 1104 59 1.258054124870057 1104 60 1.1481415471173122 1104 61 1.2255572662127179 1104 62 1.1676149591140899 1104 63 1.2454917593773516 1104 64 1.2624209469151637 1104 65 1.2374191467206117 1104 66 1.2605630531465906 1104 67 1.2636318232874402 1104 68 1.3062646313054458 1104 69 1.2782680825997161 1104 70 1.298381071577694 1104 71 1.2785472376905673 1104 72 1.2821099820589872 1104 73 1.2511762022301411 1104 74 1.2585178338399068 1104 75 1.2932845490074172 1105 1 0.33899003603753997 1105 2 0.4066099061402646 1105 3 0.32138717328188804 1105 4 0.4225101529470905 1105 5 0.3282178621597538 1105 6 0.3995852312937629 1105 7 0.42225605897841945 1105 8 0.370005730327712 1105 9 0.45382895310417126 1105 10 0.5839309932216423 1105 11 0.5808657760666875 1105 12 0.5378932650422712 1105 13 0.5703391688814534 1105 14 0.6276957661475251 1105 15 0.5860384224253536 1105 16 0.6583810735761872 1105 17 0.6047111905354257 1105 18 0.5624456534375426 1105 19 0.7006564559270885 1105 20 0.702617457442166 1105 21 0.65835025180548 1105 22 0.6758849836516703 1105 23 0.7201618171883197 1105 24 0.6711601603712225 1105 25 0.7111006595440514 1105 26 0.718812091513918 1105 27 0.6975799579890705 1105 28 0.7626181956010285 1105 29 0.7371176395871473 1105 30 0.7428712535832034 1105 31 0.7279443570227642 1105 32 0.6522714584184328 1105 33 0.6718502450995913 1105 34 0.7782526831936281 1105 35 0.7209559694038177 1105 36 0.7578841170262862 1105 37 0.7348479559880232 1105 38 0.6522530897036276 1105 39 0.7496340881075341 1105 40 0.761793546561421 1105 41 0.7109963860912885 1105 42 0.7611086210666613 1105 43 0.8576428894130572 1105 44 0.9310473772578405 1105 45 0.8289346025526427 1105 46 0.8449363928062326 1105 47 0.9132575885366521 1105 48 0.770275221111298 1105 49 0.7848782152200968 1105 50 0.7500965426944788 1105 51 0.7416364086003924 1105 52 0.6832271593824473 1105 53 0.6512301867264298 1105 54 0.7266483926327894 1105 55 0.7499436769340448 1105 56 0.8205746960408149 1105 57 0.7666476714998538 1105 58 0.6444151103158049 1105 59 0.7207478295429297 1105 60 0.805293502561689 1105 61 0.8143764341021509 1105 62 0.7615301660996892 1105 63 0.7910477635909317 1105 64 0.8380008945371955 1105 65 0.8457907778530327 1105 66 0.8027489043616924 1105 67 0.855449420721456 1105 68 0.9065550859575069 1105 69 0.8437626701767371 1105 70 0.8728857634056197 1105 71 0.8745068094133543 1105 72 0.848267159075295 1105 73 0.9177806356874143 1105 74 0.8728203202965652 1105 75 0.8950107474137425 1106 1 0.39565705210954505 1106 2 0.4445134120162635 1106 3 0.42495660753944975 1106 4 0.3379517571877386 1106 5 0.35103045784273235 1106 6 0.3891238432694806 1106 7 0.5161796143142467 1106 8 0.5261250498359331 1106 9 0.583203831964255 1106 10 0.6067734904122304 1106 11 0.5482259334794648 1106 12 0.5817892326336178 1106 13 0.3916468353618825 1106 14 0.5550497392336595 1106 15 0.51976197104905 1106 16 0.5395027327202868 1106 17 0.6915204416848795 1106 18 0.5532679604205342 1106 19 0.6487509130524671 1106 20 0.6218644997801874 1106 21 0.6517152218455777 1106 22 0.7590272159200779 1106 23 0.6957587865577963 1106 24 0.6988338763029063 1106 25 0.675179375983582 1106 26 0.6777669684017624 1106 27 0.7529083179390597 1106 28 0.7238147615329993 1106 29 0.6844049408123496 1106 30 0.6334851444708605 1106 31 0.6469904420098729 1106 32 0.7199899090753306 1106 33 0.6883010270702172 1106 34 0.7510329231537867 1106 35 0.7535502759497845 1106 36 0.7755393494648573 1106 37 0.8178698430045301 1106 38 0.679462020149245 1106 39 0.7771693109007529 1106 40 0.7906980662057137 1106 41 0.6656713925557796 1106 42 0.6248745803033273 1106 43 0.6845963966713953 1106 44 0.7326536093677589 1106 45 0.7240188242699901 1106 46 0.716340061976247 1106 47 0.7696465982061085 1106 48 0.7168423358398002 1106 49 0.743108433321467 1106 50 0.6762424800886322 1106 51 0.6607934137659228 1106 52 0.5963396616426166 1106 53 0.6433285008136451 1106 54 0.7548617032611844 1106 55 0.7482755867732749 1106 56 0.6462450996690308 1106 57 0.6383178669633054 1106 58 0.6238233945931739 1106 59 0.6712660089942588 1106 60 0.618211301284397 1106 61 0.6079978548473335 1106 62 0.6610441353891758 1106 63 0.6526249620392122 1106 64 0.6593226431862362 1106 65 0.6733186601772516 1106 66 0.6486826615448891 1106 67 0.6865804162324594 1106 68 0.6888167428514222 1106 69 0.735528785536971 1106 70 0.7618518557612859 1106 71 0.7467862711950843 1106 72 0.8432672499995313 1106 73 0.7489299638903546 1106 74 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-0.8220686259012737 2214 22 -0.8238140000053562 2214 23 -0.7875931645578547 2214 24 -0.9568593404255097 2214 25 -0.9097394620592709 2214 26 -0.8213154456494678 2214 27 -0.9008345879808104 2214 28 -1.038591406240954 2214 29 -0.8759448100925056 2214 30 -0.8768581011852845 2214 31 -0.8328079671175885 2214 32 -0.8661375216959826 2214 33 -0.8306419511656671 2214 34 -0.8361849682223976 2214 35 -0.8214214182364756 2214 36 -0.8583733567491976 2214 37 -0.8950092989199092 2214 38 -0.8196213583815961 2214 39 -0.857660648682895 2214 40 -0.9482419800121491 2214 41 -0.991891584304561 2214 42 -1.0190872718008421 2214 43 -0.9921188837586996 2214 44 -0.9753106442241304 2214 45 -0.8786367273345235 2214 46 -0.9498376135267819 2214 47 -0.9264132165579326 2214 48 -0.9728715689097953 2214 49 -0.8426133173699952 2214 50 -0.8764626723743092 2214 51 -0.952297773990054 2214 52 -0.9024212148604889 2214 53 -0.9618752724434501 2214 54 -1.0338233079407502 2214 55 -0.9920449623495315 2214 56 -1.054374209319235 2214 57 -1.0334675258133643 2214 58 -0.9529425642518845 2214 59 -1.057283897903524 2214 60 -0.9883892047414662 2214 61 -0.9935534673743902 2214 62 -1.017162064232565 2214 63 -0.996904864126968 2214 64 -1.003472535799233 2214 65 -0.9718343242485972 2214 66 -0.9840681793150452 2214 67 -1.0200506272134255 2214 68 -0.9109390509106454 2214 69 -0.8873409616908816 2214 70 -0.8872083686072685 2214 71 -0.912645534575482 2214 72 -0.9336576284793594 2214 73 -1.0401119380261967 2214 74 -0.993119799874222 2214 75 -0.9237757111016869 2215 1 -1.444013474365601 2215 2 -1.3995194296133207 2215 3 -1.1783519657853319 2215 4 -2.2816783427314746 2215 5 -1.6856039116765302 2215 6 -1.5386996570497686 2215 7 -1.3217207454480615 2215 8 -1.4113696743975055 2215 9 -1.5582675955910616 2215 10 -1.4920262917210962 2215 11 -1.4402665298890724 2215 12 -1.4990878882937295 2215 13 -1.4744488123140513 2215 14 -1.7579278300750403 2215 15 -1.8694822181351682 2215 16 -1.7779594803368255 2215 17 -1.7519964749481858 2215 18 -1.8010949359331008 2215 19 -1.8541041588377212 2215 20 -1.9073285134304712 2215 21 -1.6950106478014106 2215 22 -1.6147942989119883 2215 23 -1.6072809682979212 2215 24 -1.7768970439651426 2215 25 -1.7033697792156985 2215 26 -1.731020043954814 2215 27 -1.6101763719178237 2215 28 -1.6036298245825904 2215 29 -1.7723975668684773 2215 30 -1.7317800802498056 2215 31 -1.7176774621498225 2215 32 -1.7569832552969373 2215 33 -1.7744755165049142 2215 34 -1.8744067471236079 2215 35 -1.8419985269320804 2215 36 -1.7954553093144767 2215 37 -1.7909786657628644 2215 38 -1.7849595247391221 2215 39 -1.6800164423015858 2215 40 -1.7119938547655877 2215 41 -1.7329015973460216 2215 42 -1.7204602167294567 2215 43 -1.8672983265656953 2215 44 -1.8057861447683763 2215 45 -1.8516199620864384 2215 46 -1.8158628711977443 2215 47 -1.8399551170511117 2215 48 -1.9049238095280856 2215 49 -1.9405863551759879 2215 50 -1.8485864882080563 2215 51 -1.795271870403127 2215 52 -1.7072341045752637 2215 53 -1.6764463315449802 2215 54 -1.6823468495555716 2215 55 -1.7897279911997188 2215 56 -1.9009584465317388 2215 57 -1.8336262637411949 2215 58 -1.7675079224401955 2215 59 -1.6900607608472598 2215 60 -1.6949186944341008 2215 61 -1.8075392327191793 2215 62 -1.6899688394678698 2215 63 -1.8258977209202172 2215 64 -1.7486529043795223 2215 65 -1.694403445789753 2215 66 -1.746395107059783 2215 67 -1.7064704893450902 2215 68 -1.6671449356408061 2215 69 -1.6655518713509707 2215 70 -1.6471423817870061 2215 71 -1.7012166038036334 2215 72 -1.902880229574773 2215 73 -1.8283396397511638 2215 74 -1.7432392096271734 2215 75 -1.7240948809787113 2216 1 -0.8701732708832708 2216 2 -0.8457339227105969 2216 3 -0.9946531688337323 2216 4 -0.9371745872189834 2216 5 -0.9677111611605298 2216 6 -1.0747351580638664 2216 7 -0.9838488016547089 2216 8 -1.1397067739466635 2216 9 -1.4542275030941454 2216 10 -1.859005417198695 2216 11 -1.8931852522953037 2216 12 -1.5387586592443014 2216 13 -1.7235806424687041 2216 14 -1.7454175929913731 2216 15 -1.5364205134086362 2216 16 -1.6138665555747238 2216 17 -1.922182858422346 2216 18 -1.6311648956921232 2216 19 -1.9066695206346438 2216 20 -1.833187112913663 2216 21 -1.9672999979911765 2216 22 -2.05784679590127 2216 23 -2.108893235143171 2216 24 -2.1216516299530994 2216 25 -2.015530400069629 2216 26 -2.194535933333384 2216 27 -2.1138270346193195 2216 28 -2.092165937548593 2216 29 -2.067048273170869 2216 30 -2.0557093290878896 2216 31 -2.0299472642439724 2216 32 -1.9668714458315861 2216 33 -1.8569243207054313 2216 34 -1.9481634001093227 2216 35 -1.904045259223473 2216 36 -2.0076786896931083 2216 37 -2.016103116267999 2216 38 -2.0323399088150964 2216 39 -1.9502070068802837 2216 40 -1.9670998318048802 2216 41 -1.95089594011111 2216 42 -1.9113723497435657 2216 43 -1.9069522370690137 2216 44 -1.958726760698049 2216 45 -1.9905305022715396 2216 46 -1.8317647071680057 2216 47 -2.001042550748572 2216 48 -1.9895137831391594 2216 49 -1.9618301249543535 2216 50 -1.9213082462250597 2216 51 -1.8649560631288438 2216 52 -1.875778330602337 2216 53 -1.9861175127306439 2216 54 -2.124390655073782 2216 55 -2.041183985682949 2216 56 -2.154785714363914 2216 57 -2.096974278908583 2216 58 -2.090405319065429 2216 59 -2.156640598000397 2216 60 -2.0759457584231136 2216 61 -2.0475656338169763 2216 62 -2.113452943860601 2216 63 -2.03701708207608 2216 64 -1.9402425696341368 2216 65 -1.8961625739320844 2216 66 -2.0043550817182805 2216 67 -1.961756883909196 2216 68 -2.105439528964311 2216 69 -2.2100702893670636 2216 70 -2.074133495006805 2216 71 -2.0718271331278864 2216 72 -2.075807384352462 2216 73 -2.104183931122318 2216 74 -2.127839256550047 2216 75 -2.0710476254049013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 71404.0 3 26751.0 4 15788.0 5 10092.0 6 7479.0 7 6722.0 8 6527.0 9 6853.0 10 7497.0 11 7987.0 12 8542.0 13 9060.0 14 9590.0 15 10009.0 16 10791.0 17 11765.0 18 12456.0 19 13762.0 20 15816.0 21 18146.0 22 22878.0 23 27761.0 24 29274.0 25 30666.0 26 31939.0 27 33731.0 28 38673.0 29 46314.0 30 53187.0 31 61020.0 32 72482.0 33 90938.0 34 118247.0 35 158174.0 36 218758.0 37 337623.0 38 569295.0 39 901307.0 40 240255.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.14587184926672 18.823454989482087 14.808561518428398 25.222111642822796 2 15.030810146570813 20.0541403731649 38.6134246754249 26.301624804839392 3 18.028967149749278 24.702297570657954 29.453843213417414 27.81489206617535 4 12.352601681733159 16.674918614790148 36.40541573473147 34.567063968745224 5 13.706306378566898 37.80241489277954 34.004417450563615 14.486861278089949 6 32.37204768840031 36.84818091751228 16.946256702914475 13.83351469117294 7 28.18887081658165 32.35061080869105 21.06613880620454 18.394379568522755 8 26.629744115858085 35.02132738915294 19.2633122894386 19.085616205550377 9 26.267422588213773 16.073467783169203 19.081575510897302 38.57753411771972 10 14.716767505008768 29.647125669381847 32.85511191170913 22.780994913900255 11 34.6564829304085 23.542637766575087 22.897912516117387 18.902966786899025 12 23.499013796158394 25.280627652864567 29.862947523973265 21.357411027003774 13 28.006823651443653 22.22968127975248 25.942054212562148 23.821440856241715 14 21.984052000833742 21.498563219244012 27.266232890907393 29.25115188901485 15 23.549175819364468 29.15572948839385 24.073358918637485 23.221735773604195 16 22.909335206387222 28.7049862934967 25.55940761306158 22.826270887054495 17 21.894618418103747 28.37742166732714 26.887546177353688 22.840413737215425 18 21.863545964558163 27.419009172133336 28.42218528303007 22.29525958027843 19 22.986510596781503 27.10124309568551 28.588351331769505 21.323894975763476 20 23.065769735580645 26.31080300541566 28.564538256006465 22.058889002997223 21 23.307036826489067 26.284593017646614 28.39921808091408 22.00915207495024 22 22.868356032479706 26.010805593120324 28.52894452828083 22.59189384611914 23 22.764909114654447 26.029331449535185 28.472223871205195 22.733535564605173 24 22.377049666947674 26.26981394189098 28.570962111587374 22.782174279573976 25 22.766925336417106 26.12239954978166 28.503825431162422 22.60684968263881 26 22.352238465676386 26.44199083975267 28.61371994627825 22.592050748292696 27 22.41012740596841 26.555734396642883 28.35104889209233 22.68308930529638 28 22.395492383050065 26.413758931320235 28.497097831188807 22.693650854440893 29 22.53198691988773 26.516355633442117 28.418067135193525 22.53359031147663 30 22.653736286507712 26.427191798593874 28.51435608262444 22.40471583227398 31 22.742033631654717 26.11428232186058 28.485863128367246 22.65782091811746 32 22.47376867639669 26.507891138411694 28.317333791607936 22.701006393583683 33 22.604023977049945 26.361012212623418 28.227767133513698 22.807196676812936 34 22.865820085655404 26.417411751157065 28.135476707149515 22.58129145603801 35 22.779670333257947 26.394293010326646 28.259345593293883 22.566691063121525 36 22.78137884098431 26.406935015542167 28.14193548004123 22.66975066343229 37 22.704790385927566 26.183899319475106 28.364523191833584 22.74678710276374 38 22.820609693592125 26.3086432467132 28.160807820118517 22.70993923957616 39 22.942052901510117 26.466841580397 27.95125368031152 22.639851837781364 40 22.83660295999753 26.2009110789768 28.373568499509677 22.588917461515994 41 22.607380136010484 26.428519698725378 28.080745540717665 22.883354624546474 42 22.8133408600791 26.45357309698877 27.93832461049981 22.79476143243232 43 22.740208157350953 26.1921142876817 28.068919993042822 22.998757561924528 44 22.74984877796569 26.407908303944716 27.792057385422968 23.05018553266663 45 22.81650446018363 26.54379100175463 27.760309945366608 22.879394592695128 46 22.926893257246512 26.28010698373994 27.66776752760957 23.125232231403977 47 22.826571820588242 26.618001067849395 27.480739089490708 23.07468802207166 48 22.72869732762781 26.771624097827306 27.507637935445423 22.99204063909946 49 23.029904086830534 26.543208410965878 27.306554729387635 23.120332772815956 50 23.121968997074685 26.60462678580809 27.29756736279263 22.975836854324594 51 22.874468093946675 26.891193493607034 27.185778483721258 23.048559928725034 52 22.946239954603918 26.828204213501657 27.030106361446098 23.195449470448327 53 22.892831312840233 26.806046017325123 27.24755806178676 23.053564608047882 54 22.796794949603168 26.960517361777825 27.26702649636139 22.97566119225762 55 22.802852259611775 26.598534695695765 27.30705891814874 23.29155412654372 56 22.81954768944563 26.909444694085792 27.19680596258308 23.07420165388549 57 22.812187821082418 26.75344016344449 27.04841307009843 23.385958945374664 58 22.74923684080882 26.66415594267002 27.365645104711433 23.22096211180972 59 22.764495000025228 26.86223943903171 27.196194362240185 23.177071198702883 60 22.733117930902058 26.915896004964523 27.476719888102323 22.874266176031092 61 22.878223416192736 26.578509777199915 27.61040657683595 22.932860229771396 62 22.78409468714675 26.797974076923808 27.313004046621643 23.1049271893078 63 22.557177617016073 26.714942666561402 27.558842681935797 23.16903703448673 64 22.781057536767435 26.594422925663743 27.381090185951944 23.243429351616875 65 22.719042397639118 26.618577974979473 27.322484337682706 23.3398952896987 66 22.68402328925907 26.821028010335613 27.32406532034604 23.170883380059276 67 22.762817831858445 26.7188766619006 27.117248978096796 23.401056528144153 68 22.788903324544304 26.653374782166555 27.460030104129658 23.097691789159487 69 22.72937592581545 26.71756110344885 27.001683125443154 23.551379845292548 70 22.593339362680045 26.63275431223448 27.326505152598173 23.4474011724873 71 22.65845720976409 26.598610684462727 27.26225297058575 23.480679135187437 72 22.65509562818011 26.943092075775915 26.979631259969615 23.422181036074356 73 22.725130397463698 26.682315747359848 27.10875270158851 23.48380115358794 74 22.645082620243713 26.745372684561286 27.05256421368586 23.55698048150914 75 22.671842493418925 26.853993607379113 27.073194618802138 23.40096928039982 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 859.0 1 596.0 2 431.0 3 537.0 4 545.0 5 459.5 6 439.0 7 882.5 8 1261.0 9 2212.0 10 3815.0 11 4469.5 12 4472.0 13 4126.0 14 3962.5 15 4474.5 16 4804.0 17 5375.5 18 6444.0 19 7702.5 20 8464.0 21 9498.5 22 12305.5 23 16167.0 24 18256.0 25 21144.5 26 28010.5 27 35439.5 28 38891.0 29 43966.5 30 53485.0 31 63306.5 32 68685.0 33 74903.5 34 86654.5 35 99342.5 36 106498.0 37 110286.5 38 115917.0 39 121010.5 40 124262.0 41 129124.5 42 137525.5 43 142950.0 44 144836.0 45 150240.0 46 158269.0 47 162631.0 48 164368.0 49 166274.0 50 166914.5 51 162790.0 52 159931.0 53 155483.5 54 147949.5 55 139298.5 56 133734.0 57 128117.0 58 112998.5 59 95127.0 60 86757.0 61 78980.5 62 62061.5 63 45435.0 64 37951.0 65 31324.5 66 20198.5 67 13572.5 68 11446.0 69 9254.5 70 5710.0 71 3434.5 72 2512.0 73 2260.0 74 1707.5 75 1020.0 76 633.0 77 523.0 78 299.5 79 135.5 80 85.0 81 73.5 82 40.0 83 15.5 84 13.0 85 9.0 86 7.5 87 5.5 88 1.0 89 1.0 90 1.5 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07259110168422633 2 0.01599615854775061 3 0.002581940247967167 4 0.13939509591611246 5 0.09983502292139712 6 0.07024658122917568 7 0.029440054321648618 8 0.026294242065504715 9 0.049294284504292706 10 0.028668439994669923 11 0.01564002885837583 12 0.10749181124295494 13 0.09001177898947607 14 0.04786976574679357 15 0.02071487693196647 16 0.03220005941430318 17 0.022376815482382118 18 0.01955745544149843 19 0.013948412833845614 20 0.013087766084523228 21 0.027421986081858193 22 0.01670841792650017 23 0.014215510100876703 24 0.021338103888372338 25 0.01564002885837583 26 0.032378124258990566 27 0.028401342727638838 28 0.03608780852331121 29 0.05039235104653161 30 0.03445554744701013 31 0.021041329147226683 32 0.011870989645826056 33 0.01495744695374083 34 0.014185832626762138 35 0.006440011882860636 36 0.010030986250723017 37 0.007360013580412154 38 0.0028490375149982533 39 0.008280015277963675 40 0.010891633000045408 41 0.012078731964628013 42 0.007152271261610199 43 0.011188407741191059 44 0.010120018673066711 45 0.0056683975558819415 46 0.002225810558592386 47 0.0050451705994760745 48 0.005727752504111072 49 0.0015729061280719523 50 1.7806484468739083E-4 51 0.002166455610363255 52 0.0031161347820293397 53 5.935494822913028E-5 54 5.935494822913028E-5 55 5.935494822913028E-4 56 0.008339370226192805 57 0.0028787149891128187 58 3.858071634893468E-4 59 0.007181948735724765 60 0.0015432286539573872 61 0.001068389068124345 62 0.02442456119628711 63 0.00952646919077541 64 0.012138086912857142 65 0.019141970803894515 66 0.01851874384748865 67 0.017806484468739087 68 0.017390999831135172 69 0.006885173994579113 70 0.014304542523220398 71 0.008131627907390847 72 0.017005192667645826 73 0.0072709811580684595 74 0.019320035648581906 75 0.001454196231613692 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3369559.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.005784871070105 #Duplication Level Percentage of deduplicated Percentage of total 1 72.66485341088676 37.78992734174422 2 13.189978387335103 13.719103569316271 3 5.440563661122379 8.48822350013076 4 2.8157628259109626 5.857438230891277 5 1.6677632591106335 4.336666863459118 6 1.0178717237646973 3.176113073547128 7 0.6737355143340057 2.4526700952917846 8 0.46429143995057864 1.9316672594839324 9 0.3460517531418681 1.6197023725337383 >10 1.6056597001471964 14.347730095936518 >50 0.08056874678329788 2.8405370771229226 >100 0.03140830552848034 2.844122629382675 >500 0.0013192021418399708 0.4237771601346926 >1k 1.7206984218254203E-4 0.17232073102501272 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.967747411456514E-5 0.0 2 0.0 0.0 0.0 2.967747411456514E-5 0.0 3 0.0 0.0 0.0 2.967747411456514E-5 0.0 4 0.0 0.0 0.0 2.967747411456514E-5 0.0 5 0.0 0.0 0.0 2.967747411456514E-5 0.0 6 0.0 0.0 0.0 2.967747411456514E-5 0.0 7 0.0 0.0 0.0 2.967747411456514E-5 0.0 8 0.0 0.0 0.0 2.967747411456514E-5 0.0 9 0.0 0.0 0.0 2.967747411456514E-5 0.0 10 0.0 0.0 0.0 2.967747411456514E-5 2.967747411456514E-5 11 0.0 0.0 0.0 2.967747411456514E-5 2.967747411456514E-5 12 0.0 0.0 0.0 2.967747411456514E-5 5.935494822913028E-5 13 0.0 0.0 0.0 2.967747411456514E-5 5.935494822913028E-5 14 0.0 0.0 0.0 2.967747411456514E-5 5.935494822913028E-5 15 0.0 0.0 0.0 5.935494822913028E-5 5.935494822913028E-5 16 0.0 0.0 0.0 5.935494822913028E-5 5.935494822913028E-5 17 0.0 0.0 0.0 5.935494822913028E-5 5.935494822913028E-5 18 0.0 0.0 0.0 5.935494822913028E-5 5.935494822913028E-5 19 0.0 0.0 0.0 5.935494822913028E-5 5.935494822913028E-5 20 0.0 0.0 0.0 1.1870989645826056E-4 5.935494822913028E-5 21 0.0 0.0 0.0 1.483873705728257E-4 5.935494822913028E-5 22 0.0 0.0 0.0 1.483873705728257E-4 5.935494822913028E-5 23 0.0 0.0 0.0 1.7806484468739086E-4 5.935494822913028E-5 24 0.0 0.0 0.0 2.967747411456514E-4 5.935494822913028E-5 25 0.0 0.0 0.0 3.8580716348934684E-4 5.935494822913028E-5 26 0.0 0.0 0.0 7.122593787495634E-4 5.935494822913028E-5 27 0.0 0.0 0.0 0.001068389068124345 5.935494822913028E-5 28 0.0 0.0 0.0 0.001721293498644778 5.935494822913028E-5 29 0.0 0.0 0.0 0.0026412951961962976 5.935494822913028E-5 30 0.0 0.0 0.0 0.005015493125361509 5.935494822913028E-5 31 0.0 0.0 0.0 0.010327760991868669 5.935494822913028E-5 32 0.0 0.0 0.0 0.016203900866552566 5.935494822913028E-5 33 0.0 0.0 0.0 0.024543271092745372 5.935494822913028E-5 34 0.0 0.0 0.0 0.03401038533529165 5.935494822913028E-5 35 0.0 0.0 0.0 0.047246538790387704 5.935494822913028E-5 36 0.0 0.0 0.0 0.061877533528868316 5.935494822913028E-5 37 0.0 0.0 0.0 0.08253305551260566 5.935494822913028E-5 38 0.0 0.0 0.0 0.10621567985602864 8.903242234369543E-5 39 0.0 0.0 0.0 0.1302544338888264 8.903242234369543E-5 40 0.0 0.0 0.0 0.15429318792162416 8.903242234369543E-5 41 0.0 0.0 0.0 0.17732290783452673 8.903242234369543E-5 42 0.0 0.0 0.0 0.20189585640138666 8.903242234369543E-5 43 0.0 0.0 0.0 0.22890235784564092 8.903242234369543E-5 44 0.0 0.0 0.0 0.2633579052926511 8.903242234369543E-5 45 0.0 0.0 0.0 0.2994160363418477 8.903242234369543E-5 46 0.0 0.0 0.0 0.3372251383638037 8.903242234369543E-5 47 0.0 0.0 0.0 0.37574649976450925 8.903242234369543E-5 48 0.0 0.0 0.0 0.41536592770745373 8.903242234369543E-5 49 0.0 0.0 0.0 0.45798278053596925 8.903242234369543E-5 50 0.0 0.0 0.0 0.502498991707817 8.903242234369543E-5 51 0.0 0.0 0.0 0.5439287455717499 8.903242234369543E-5 52 0.0 0.0 0.0 0.5857739840732867 8.903242234369543E-5 53 0.0 0.0 0.0 0.6288656764876354 8.903242234369543E-5 54 0.0 0.0 0.0 0.6775960889837512 8.903242234369543E-5 55 0.0 0.0 0.0 0.7315794143981452 8.903242234369543E-5 56 0.0 0.0 0.0 0.7893911339733182 8.903242234369543E-5 57 0.0 0.0 0.0 0.8512389900280719 8.903242234369543E-5 58 0.0 0.0 0.0 0.9186068562681348 8.903242234369543E-5 59 0.0 0.0 0.0 0.9843721389060112 8.903242234369543E-5 60 0.0 0.0 0.0 1.053995493178781 8.903242234369543E-5 61 0.0 0.0 0.0 1.1294356323780055 1.483873705728257E-4 62 0.0 0.0 0.0 1.2042228671467097 1.483873705728257E-4 63 0.0 0.0 0.0 1.28405527251489 1.483873705728257E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4210 0.0 44.13639 1 GTATCAA 8680 0.0 34.76173 1 GTGGTAT 1615 0.0 26.781944 1 CGTGCCG 410 0.0 25.262308 10 TATCAAC 11975 0.0 24.977808 2 ATCAACG 12255 0.0 24.382532 3 TCAACGC 12335 0.0 24.224396 4 CAACGCA 12495 0.0 23.964064 5 TGGTATC 1815 0.0 23.433771 2 AACGCAG 13155 0.0 22.834072 6 GTATACG 150 7.134986E-7 20.761364 1 GTACATG 12200 0.0 19.825401 1 ACGCAGA 15350 0.0 19.432764 7 TACATGG 12315 0.0 18.925144 2 CGCAGAG 15665 0.0 18.865963 8 CGAATCG 370 0.0 18.641748 37 ACATGGG 12490 0.0 18.413553 3 GAGTACT 9605 0.0 18.18816 12 AGAGTAC 15420 0.0 18.180561 11 GTGCCGA 535 0.0 18.06924 11 >>END_MODULE