FastQCFastQC Report
Mon 6 Feb 2017
SRR2077511_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077511_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3850809
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38300.036.3309021
GTATCAA77400.034.840151
TCAACGC97100.027.3594784
CAACGCA97900.027.1736245
ATCAACG98300.027.0605833
TATCAAC99300.026.9951322
AACGCAG104200.025.3099336
ACGCAGA120100.021.759647
GTGGTAT20950.021.4254151
CGCAGAG122300.021.3148848
GAGTACT71400.020.42951412
AGAGTAC118300.020.40465211
TGGTATC21700.019.237092
CAGAGTA128200.019.07028410
GCAGAGT133550.018.9518289
AGTACTT78600.018.82207913
GTACATG102950.018.3120171
GTACTTT79200.018.28808614
TACATGG102600.017.8886662
ACATGGG108950.016.466923
Produced by FastQC (version 0.11.5)