FastQCFastQC Report
Mon 6 Feb 2017
SRR2077511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3850809
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42300.041.058631
GTATCAA84650.034.5359081
TATCAAC118050.024.3967322
TCAACGC119650.023.927884
ATCAACG120850.023.69093
CAACGCA120900.023.5916485
AACGCAG126150.022.5515696
ACGCAGA146000.019.4386947
CGCAGAG149450.018.8513458
GTGGTAT19600.018.7107371
GCACCGT6800.018.2780726
GTACATG96400.018.1599481
AGAGTAC148750.018.01162311
GAGTACT106550.017.95165312
TATACCG2701.5097612E-1017.9047745
TACATGG96900.017.5547242
AGTACTT112500.017.11673413
CGTGCCG4900.016.91076510
CAGAGTA160800.016.8121710
GTACTTT113650.016.75481214
Produced by FastQC (version 0.11.5)