FastQCFastQC Report
Mon 6 Feb 2017
SRR2077510_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077510_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903428
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14850.037.8884541
GTATCAA31500.035.9425661
TATCAAC41450.026.4616322
CAACGCA41150.026.4065655
TCAACGC41250.026.3418544
ATCAACG41650.026.2545153
AACGCAG42900.025.2310246
GTACCGA1854.8930815E-1022.3600126
GTACATG47350.021.5783141
TACATGG47800.021.5753232
GCGCGAA800.00290242121.55955521
ACGCAGA50250.021.406137
CGCAGAG51950.020.7083668
ACATGGG47900.020.1642593
AGAGTAC52200.019.29011711
TAAGACG900.005740295619.164054
GAGTACT34400.018.24416512
GCAGAGT57650.018.1833529
CAGAGTA56400.018.03609710
CGAATCG1351.5732345E-417.895437
Produced by FastQC (version 0.11.5)