FastQCFastQC Report
Mon 6 Feb 2017
SRR2077510_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077510_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903428
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25300.132918082533198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19450.040.7321471
GTATCAA44050.037.6193281
GCGTATC1004.900576E-727.6765141
TATCAAC60400.027.1331142
TCAACGC61500.026.649214
ATCAACG61750.026.5420173
CAACGCA62600.026.1437455
AACGCAG64800.025.1975636
ACGCAGA74050.021.8169087
CGCAGAG74850.021.4459368
GAGTACT57750.020.20664812
GTACATG44100.020.161181
AGAGTAC75150.020.07622111
AGTACTT62000.018.98058113
TACATGG45250.018.949222
CAGAGTA80250.018.88539910
GTACTTT62500.018.71141614
TATACTG7800.018.5916755
GCAGAGT84800.018.4415599
CCTATAC4550.018.2386383
Produced by FastQC (version 0.11.5)