##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077510_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1903428 Sequences flagged as poor quality 0 Sequence length 75 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.981763954297193 33.0 31.0 34.0 28.0 34.0 2 31.08263827158159 33.0 31.0 34.0 27.0 34.0 3 31.148164784798794 33.0 31.0 34.0 27.0 34.0 4 34.52416797483278 37.0 35.0 37.0 31.0 37.0 5 34.246534673231665 37.0 35.0 37.0 30.0 37.0 6 34.227216894991564 37.0 35.0 37.0 30.0 37.0 7 34.07336027420002 37.0 35.0 37.0 30.0 37.0 8 34.0329694635153 37.0 35.0 37.0 30.0 37.0 9 35.51894371628451 39.0 37.0 39.0 30.0 39.0 10 35.414066620854584 39.0 35.0 39.0 29.0 39.0 11 35.40195216209912 39.0 37.0 39.0 29.0 39.0 12 35.299199654518056 39.0 35.0 39.0 28.0 39.0 13 35.23753091790181 39.0 35.0 39.0 28.0 39.0 14 36.48573258352825 40.0 37.0 41.0 28.0 41.0 15 36.52868456279933 40.0 37.0 41.0 29.0 41.0 16 36.51782468262524 40.0 37.0 41.0 29.0 41.0 17 36.51076951689268 40.0 37.0 41.0 28.0 41.0 18 36.516431406914265 40.0 37.0 41.0 29.0 41.0 19 36.56146857144058 40.0 37.0 41.0 28.0 41.0 20 36.52805622277281 40.0 37.0 41.0 28.0 41.0 21 36.503187932509135 40.0 37.0 41.0 28.0 41.0 22 36.4843682030526 40.0 37.0 41.0 28.0 41.0 23 36.453885831247625 40.0 37.0 41.0 28.0 41.0 24 36.43715338851798 40.0 37.0 41.0 28.0 41.0 25 36.40747325351944 40.0 37.0 41.0 28.0 41.0 26 36.338440434836514 40.0 37.0 41.0 28.0 41.0 27 36.28868861863963 40.0 36.0 41.0 28.0 41.0 28 36.26684119388808 40.0 36.0 41.0 28.0 41.0 29 36.214067986811166 40.0 36.0 41.0 28.0 41.0 30 36.136570965647245 40.0 36.0 41.0 27.0 41.0 31 36.048259245949936 40.0 36.0 41.0 27.0 41.0 32 35.979518531827836 40.0 36.0 41.0 27.0 41.0 33 35.91799427138825 40.0 36.0 41.0 27.0 41.0 34 35.84609714683192 40.0 35.0 41.0 27.0 41.0 35 35.77218996463223 40.0 35.0 41.0 27.0 41.0 36 35.71039671582009 40.0 35.0 41.0 26.0 41.0 37 35.66038011419397 40.0 35.0 41.0 26.0 41.0 38 35.602880697352354 40.0 35.0 41.0 26.0 41.0 39 35.53272569280267 40.0 35.0 41.0 25.0 41.0 40 35.47093979914134 40.0 35.0 41.0 25.0 41.0 41 35.4252291129478 40.0 35.0 41.0 25.0 41.0 42 35.36587041905447 40.0 35.0 41.0 25.0 41.0 43 35.26987729506974 40.0 35.0 41.0 24.0 41.0 44 35.1501102221886 40.0 35.0 41.0 24.0 41.0 45 35.06108715433418 40.0 35.0 41.0 24.0 41.0 46 34.92973676965979 40.0 35.0 41.0 23.0 41.0 47 34.78555164681827 39.0 35.0 41.0 23.0 41.0 48 34.68255169094917 39.0 35.0 41.0 22.0 41.0 49 34.594136473772586 39.0 35.0 41.0 21.0 41.0 50 34.592574029592924 39.0 35.0 41.0 20.0 41.0 51 34.52969852287557 39.0 34.0 41.0 20.0 41.0 52 34.47313793849833 39.0 34.0 41.0 20.0 41.0 53 34.382043870322384 39.0 34.0 41.0 19.0 41.0 54 34.353388728126305 39.0 34.0 41.0 19.0 41.0 55 34.27515566651326 39.0 34.0 41.0 18.0 41.0 56 34.15844570953038 39.0 34.0 41.0 18.0 41.0 57 34.087606150587256 39.0 34.0 41.0 18.0 41.0 58 33.98966233553357 39.0 34.0 41.0 17.0 41.0 59 33.89350582212724 38.0 34.0 41.0 15.0 41.0 60 33.86452652792751 38.0 34.0 41.0 15.0 41.0 61 33.755204294567484 38.0 34.0 41.0 13.0 41.0 62 33.61567813439752 38.0 34.0 41.0 13.0 41.0 63 33.48082196962533 37.0 34.0 40.0 10.0 41.0 64 33.33541799322065 37.0 34.0 40.0 8.0 41.0 65 33.203450301245965 37.0 34.0 40.0 7.0 41.0 66 33.00887609092648 37.0 33.0 40.0 2.0 41.0 67 32.81613068631963 37.0 33.0 40.0 2.0 41.0 68 32.60776031454828 36.0 33.0 39.0 2.0 41.0 69 32.44147086204469 36.0 33.0 39.0 2.0 41.0 70 32.2511479288946 36.0 33.0 39.0 2.0 41.0 71 32.072996719602735 36.0 33.0 39.0 2.0 41.0 72 31.87587867783809 35.0 33.0 39.0 2.0 41.0 73 31.669657060839707 35.0 33.0 39.0 2.0 40.0 74 31.4071979607319 35.0 32.0 38.0 2.0 40.0 75 31.182033678184833 35.0 32.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4675490822482722 1101 2 0.3847419243505996 1101 3 0.4070205281815653 1101 4 0.41686648753136524 1101 5 0.4412958760641956 1101 6 0.44059653942326804 1101 7 0.5123897346272415 1101 8 0.5112126720838148 1101 9 0.7461635934091788 1101 10 0.5711932426848207 1101 11 0.6724852008699145 1101 12 0.6565177412943513 1101 13 0.6671215225658997 1101 14 0.7196425009622587 1101 15 0.7039568464914225 1101 16 0.680748627036543 1101 17 0.7207485804750746 1101 18 0.7077007904112449 1101 19 0.758322698423548 1101 20 0.6731251153293556 1101 21 0.7473552520031532 1101 22 0.7929188370066882 1101 23 0.8391447453324119 1101 24 0.9476313547482462 1101 25 0.9373364290331878 1101 26 0.9688173630342689 1101 27 0.9046765248383579 1101 28 0.8307598715406144 1101 29 0.9152283841174906 1101 30 0.9354301409381236 1101 31 0.9828885835281511 1101 32 0.8857512103004197 1101 33 0.9298820356561563 1101 34 0.9040622001801708 1101 35 1.0277408252731846 1101 36 0.9842198565398448 1101 37 1.1059378887805877 1101 38 1.0912836339383603 1101 39 1.085748390577109 1101 40 1.1595273261756844 1101 41 1.135058050585073 1101 42 1.0405279519272028 1101 43 1.2124535011143749 1101 44 1.3291257297591343 1101 45 1.2389763723455118 1101 46 1.1682264764149295 1101 47 1.160131347803521 1101 48 1.0692944622863152 1101 49 1.0613412277219325 1101 50 1.0420558131466535 1101 51 1.0714449751230077 1101 52 1.0389806311268828 1101 53 1.0445029537700776 1101 54 1.0801374419880005 1101 55 1.1687908088023065 1101 56 1.1874330889369276 1101 57 1.0172432954905304 1101 58 0.9505509259171276 1101 59 0.9722628175110941 1101 60 0.990803878506874 1101 61 0.9780980564482036 1101 62 0.9186699524912356 1101 63 0.9392681818302151 1101 64 0.9589788187697152 1101 65 0.9099602440534085 1101 66 0.9791892397253434 1101 67 0.9601562102212071 1101 68 1.0921534949595255 1101 69 1.0705290101423017 1101 70 1.0238550847898438 1101 71 1.0583517417845698 1101 72 1.098253242929946 1101 73 1.0169422122128466 1101 74 1.049811068371806 1101 75 1.0203729600397615 1102 1 0.6647170511983909 1102 2 0.7001133773956454 1102 3 0.7161733984306906 1102 4 0.6605797679536707 1102 5 0.7594805072751427 1102 6 0.778925067320408 1102 7 0.8744028127908905 1102 8 0.8950426354641365 1102 9 1.0084588500645566 1102 10 1.091714811818548 1102 11 1.109586856849866 1102 12 1.0157287071153078 1102 13 1.0661165818807632 1102 14 1.2738631903630449 1102 15 1.2089895419005359 1102 16 1.205840790999254 1102 17 1.1788380046219302 1102 18 1.1904682298412368 1102 19 1.2347326174995956 1102 20 1.2843474994404716 1102 21 1.1701787172861842 1102 22 1.2642913474526196 1102 23 1.3055372254907738 1102 24 1.2724722835396776 1102 25 1.2301028643421787 1102 26 1.316435854434694 1102 27 1.3014518619873527 1102 28 1.2457503733756994 1102 29 1.2369610461242146 1102 30 1.2698553021610195 1102 31 1.4171975536073944 1102 32 1.3247262691644508 1102 33 1.412577752157965 1102 34 1.4567853148405376 1102 35 1.414566347979644 1102 36 1.4055822233145037 1102 37 1.4900973677852392 1102 38 1.4315240901781578 1102 39 1.5139018940372253 1102 40 1.4728688154305658 1102 41 1.5381679567509892 1102 42 1.3965060579910684 1102 43 1.4448759532598103 1102 44 1.528066279648577 1102 45 1.5112894777638743 1102 46 1.5773012711336634 1102 47 1.6305685912659698 1102 48 1.504944856045185 1102 49 1.5322403668418687 1102 50 1.4420722069588123 1102 51 1.4862481776542111 1102 52 1.544890395486327 1102 53 1.5595604653123445 1102 54 1.5814540700267514 1102 55 1.6299864505073458 1102 56 1.5206565837288153 1102 57 1.4969786993623373 1102 58 1.4911872927204755 1102 59 1.6040200907283193 1102 60 1.616562246011064 1102 61 1.6120398051351614 1102 62 1.649475769835476 1102 63 1.6044737499885144 1102 64 1.6148321269385448 1102 65 1.573576022277912 1102 66 1.6098628819590814 1102 67 1.583594853305371 1102 68 1.6684783711350164 1102 69 1.6039066067684544 1102 70 1.6013336754957805 1102 71 1.6763365734098699 1102 72 1.7640264468341336 1102 73 1.754422770176241 1102 74 1.8444236518521713 1102 75 1.8948840658228896 1103 1 0.4940312519009318 1103 2 0.5323979461840871 1103 3 0.5136863891844143 1103 4 0.5566995137053681 1103 5 0.5986802884835001 1103 6 0.6262375960513609 1103 7 0.6606701459186226 1103 8 0.5447343293342897 1103 9 0.7520711233446278 1103 10 0.7354157267477817 1103 11 0.8528612444563279 1103 12 0.9164027327845332 1103 13 0.8567143602145677 1103 14 0.9861762330991866 1103 15 0.9575455752546276 1103 16 0.9111140962853597 1103 17 0.8789285859133074 1103 18 0.9127704299773995 1103 19 0.9537626412214166 1103 20 0.9625558131349266 1103 21 0.9713902178127967 1103 22 0.9960508550938343 1103 23 0.9620366756022065 1103 24 0.984218562820999 1103 25 1.0752398903663618 1103 26 1.0257032709670355 1103 27 1.1069988507898003 1103 28 1.1033239973450222 1103 29 1.0239507020122929 1103 30 1.0177045298924767 1103 31 1.0977621446629229 1103 32 0.9586537149078111 1103 33 1.0347955778578353 1103 34 1.0427733394895142 1103 35 1.037596107910126 1103 36 1.0177817969811471 1103 37 0.9954249830533257 1103 38 1.0813659123556363 1103 39 1.1089313669006629 1103 40 1.084934941712909 1103 41 1.0601152023620628 1103 42 1.0807668312488303 1103 43 1.0968347009492092 1103 44 1.1648387925908423 1103 45 1.1194037770002367 1103 46 1.0712668502429352 1103 47 1.1611565550101801 1103 48 1.116504347781344 1103 49 1.1951720584620347 1103 50 1.1729802116682606 1103 51 1.1655062257146653 1103 52 1.1148499927390176 1103 53 1.0834950683354734 1103 54 1.0755290449568733 1103 55 1.1080779977405442 1103 56 1.096763683013279 1103 57 1.1086297422109723 1103 58 1.1178228475260354 1103 59 1.1094311106115953 1103 60 1.0818680597006676 1103 61 1.1129923634262582 1103 62 0.9820817373387101 1103 63 1.0001320548576018 1103 64 1.1162544665737784 1103 65 0.9768726381841475 1103 66 1.0756594251287268 1103 67 1.0419955407145736 1103 68 1.0023179510860984 1103 69 1.023597078664011 1103 70 1.0516565289422388 1103 71 1.0175549402699176 1103 72 0.9350660368713513 1103 73 0.9730677749186682 1103 74 1.0601638484155167 1103 75 1.098357189170553 1104 1 0.5481880654988274 1104 2 0.5584864149274118 1104 3 0.5249524399988417 1104 4 0.5198670886501233 1104 5 0.6058690118471333 1104 6 0.5887757087610126 1104 7 0.6235084346130648 1104 8 0.5516625725297644 1104 9 0.7559839966979567 1104 10 0.7830798169669251 1104 11 0.703021634458274 1104 12 0.7951864529630512 1104 13 0.7815425812938059 1104 14 0.8470541155353004 1104 15 0.8786783048334925 1104 16 0.9087172250718254 1104 17 0.8895595036616868 1104 18 0.8566352524898377 1104 19 0.8209181335157041 1104 20 0.9473243392670625 1104 21 1.0145044113355723 1104 22 0.9804023861236786 1104 23 0.9880121057819906 1104 24 0.9916919618981623 1104 25 0.9624867586499732 1104 26 1.0012158668277138 1104 27 1.1228866288259098 1104 28 0.933534404075921 1104 29 0.9595693802172107 1104 30 0.9042051168234764 1104 31 1.0066197935742238 1104 32 0.9941323235883388 1104 33 1.04565710939805 1104 34 1.0789290453850953 1104 35 1.027821107579328 1104 36 0.97020919616795 1104 37 1.0074766329815859 1104 38 0.9948713743109749 1104 39 0.9838865200792597 1104 40 1.0397716879413395 1104 41 0.9466490581679565 1104 42 0.960319138048348 1104 43 1.0009439740810038 1104 44 1.0878825921988025 1104 45 1.2160052480137864 1104 46 1.181528974952073 1104 47 1.1353225175486727 1104 48 1.1951663341221987 1104 49 1.1558830294406164 1104 50 1.1931487109584609 1104 51 1.1253355715087068 1104 52 1.1598090965965042 1104 53 1.1518032515335577 1104 54 1.158626377208968 1104 55 1.054366155344276 1104 56 1.2194797085925089 1104 57 1.1788527759921124 1104 58 1.2531417278015624 1104 59 1.2164332636370716 1104 60 1.105877979874272 1104 61 1.1508372208332105 1104 62 1.0985530438792566 1104 63 1.0296177293934932 1104 64 1.0873434362405021 1104 65 1.118245697578537 1104 66 1.1432099853123106 1104 67 1.1722201245228376 1104 68 1.1975098425539557 1104 69 1.1938138745311093 1104 70 1.2311439992412616 1104 71 1.2073285417957536 1104 72 1.1868433698039489 1104 73 1.1754015108810023 1104 74 1.1097919364192528 1104 75 1.146603106644843 1105 1 0.4303373832344377 1105 2 0.40729045169446465 1105 3 0.4123509252959501 1105 4 0.3713055418790887 1105 5 0.45885138154061167 1105 6 0.5477455999781711 1105 7 0.5339947973645423 1105 8 0.561599269852131 1105 9 0.6763871440464371 1105 10 0.6387807065586557 1105 11 0.6522911837454828 1105 12 0.7022406534225993 1105 13 0.7899276938971767 1105 14 0.692766608927613 1105 15 0.778135120316243 1105 16 0.7841695593255977 1105 17 0.8397690325315494 1105 18 0.8620831762604624 1105 19 0.8374909630100902 1105 20 0.7891134073539874 1105 21 0.8403313892937945 1105 22 0.825618669241571 1105 23 1.0063957564697503 1105 24 0.9366406971432681 1105 25 0.9361731433672844 1105 26 0.954855826448167 1105 27 0.8441572041750476 1105 28 0.881625515802142 1105 29 0.8956975202128206 1105 30 0.8721370949536151 1105 31 0.9072499756754766 1105 32 0.9457157068271655 1105 33 0.9272399657535857 1105 34 0.950066873354487 1105 35 0.8730049861379214 1105 36 0.9457632955796953 1105 37 0.969138947295427 1105 38 0.8938698260656608 1105 39 0.9456054805310998 1105 40 0.9969707070973755 1105 41 0.8654019229412526 1105 42 0.8576276054586387 1105 43 0.8895281941490225 1105 44 1.0318701983206395 1105 45 1.0576784265126946 1105 46 0.9704441915147086 1105 47 1.029603632295192 1105 48 0.919518434642626 1105 49 1.0533642799399772 1105 50 0.9680001014232573 1105 51 1.0483049867993515 1105 52 0.9438538584242337 1105 53 0.9837884562549277 1105 54 1.090536111464374 1105 55 0.9380378689592135 1105 56 0.918633182769419 1105 57 0.9477513747051844 1105 58 0.885832337727102 1105 59 0.9447556647604998 1105 60 1.0143525389558192 1105 61 0.9617552568398722 1105 62 0.9131430606529491 1105 63 0.930648639632615 1105 64 0.8997301903450605 1105 65 0.8411287089564965 1105 66 0.862505243041106 1105 67 0.8297912519120842 1105 68 0.8327280081046453 1105 69 0.8394472612206556 1105 70 0.8329839845379965 1105 71 0.7618849567729882 1105 72 0.8365521863919163 1105 73 0.7969147741165941 1105 74 0.8258666509437411 1105 75 0.8357295959868303 1106 1 0.2683371811047941 1106 2 0.28870214043972453 1106 3 0.34030320354129273 1106 4 0.3938932898531249 1106 5 0.33628034997533973 1106 6 0.3858967555897337 1106 7 0.5010582033256412 1106 8 0.46321482214742815 1106 9 0.6162784825996965 1106 10 0.664823099179678 1106 11 0.5258183795224127 1106 12 0.5348612642277217 1106 13 0.451521960312661 1106 14 0.5356508073231225 1106 15 0.5619045411669177 1106 16 0.5775904310791304 1106 17 0.6508707396648603 1106 18 0.6007645150484251 1106 19 0.6052056862385697 1106 20 0.6836584935201344 1106 21 0.7076510017605031 1106 22 0.7295163226697028 1106 23 0.6618097414460564 1106 24 0.6687046850653005 1106 25 0.7297930313912957 1106 26 0.6734736428828398 1106 27 0.7301984759824194 1106 28 0.7639992362664216 1106 29 0.6868846678330769 1106 30 0.671045403296155 1106 31 0.7265341014575881 1106 32 0.8065913592580642 1106 33 0.6865907368398538 1106 34 0.6875840507816804 1106 35 0.7696937481609254 1106 36 0.7944017225321005 1106 37 0.7700515482584649 1106 38 0.7010173122099559 1106 39 0.7356287062806928 1106 40 0.7933150344630988 1106 41 0.6547493076375517 1106 42 0.6384706968159648 1106 43 0.7901218422010814 1106 44 0.8347286563205429 1106 45 0.8969592569110745 1106 46 0.7600643554313109 1106 47 0.8269550155181449 1106 48 0.628861278606685 1106 49 0.6991591451765018 1106 50 0.8556124282659354 1106 51 0.8547572331925153 1106 52 0.7684426898514616 1106 53 0.7774337302291983 1106 54 0.7181630184641392 1106 55 0.7386357799349952 1106 56 0.7068501742086042 1106 57 0.6917824432764661 1106 58 0.7172353808798633 1106 59 0.6893701312076956 1106 60 0.6665719655303164 1106 61 0.7402793655894513 1106 62 0.7422800485093646 1106 63 0.7070187744511713 1106 64 0.6708068755530476 1106 65 0.6155546631688651 1106 66 0.6358031988973352 1106 67 0.640079411186413 1106 68 0.6199906315079033 1106 69 0.7337780037501176 1106 70 0.7309015702479158 1106 71 0.6532886513388192 1106 72 0.7964129276904011 1106 73 0.6821575859410842 1106 74 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1 -0.6396680425949128 2216 2 -0.6149537018925315 2216 3 -0.7704963578046105 2216 4 -0.5851565990418166 2216 5 -0.6919234640088447 2216 6 -0.7683525191040488 2216 7 -0.7690255445827958 2216 8 -0.7176681667655558 2216 9 -1.088649008134425 2216 10 -1.5011848388791194 2216 11 -1.3932971366382745 2216 12 -1.2382559027587519 2216 13 -1.1933166463732903 2216 14 -1.290560483428301 2216 15 -1.1859568065518786 2216 16 -1.1377717521967057 2216 17 -1.5464751254223117 2216 18 -1.2963113022758534 2216 19 -1.5571947810326918 2216 20 -1.464432432170284 2216 21 -1.4630868831963753 2216 22 -1.6443522583569035 2216 23 -1.6779815668387172 2216 24 -1.6301223316703997 2216 25 -1.6756629157993643 2216 26 -1.5570053421903225 2216 27 -1.5496494900101467 2216 28 -1.4406945120584567 2216 29 -1.4503850200796933 2216 30 -1.5917070123446138 2216 31 -1.4860221051027835 2216 32 -1.5431196394488254 2216 33 -1.477803107907775 2216 34 -1.4551752201641008 2216 35 -1.491127115885135 2216 36 -1.623730879568697 2216 37 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-1.572630930034876 2216 74 -1.6195144617115425 2216 75 -1.6068549765353275 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39606.0 3 15566.0 4 8974.0 5 5704.0 6 4270.0 7 3877.0 8 3687.0 9 3783.0 10 4008.0 11 4456.0 12 4725.0 13 5041.0 14 5188.0 15 5605.0 16 5936.0 17 6496.0 18 6628.0 19 7507.0 20 8505.0 21 9759.0 22 12214.0 23 15006.0 24 15964.0 25 16692.0 26 17647.0 27 18350.0 28 20829.0 29 25051.0 30 28755.0 31 32344.0 32 39103.0 33 48719.0 34 63717.0 35 86954.0 36 121197.0 37 186240.0 38 320333.0 39 525884.0 40 149108.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.137785404512215 18.597210108297176 14.352963937918522 25.912040549272085 2 14.695319408750828 20.207581402900548 38.93005148813261 26.167047700216013 3 17.587561108236116 24.794458294039305 29.765076429624067 27.85290416810052 4 12.00353551163641 16.902639269958936 37.12150427602244 33.97232094238221 5 13.415125375200091 37.64626756104692 34.6393030901553 14.299303973597693 6 31.874415782827352 37.41629068228154 17.296455845536396 13.41283768935471 7 27.56032588617317 32.18822275580136 21.900042724869184 18.351408633156286 8 25.910781606851764 35.38140736949308 19.78127368063708 18.92653734301807 9 25.927829876524132 15.8057626977882 19.334478156634745 38.93192926905292 10 14.06327021166796 29.5169547729081 33.85846550975729 22.561309505666653 11 34.426904843725204 23.37522239772332 23.534487016596938 18.663385741954535 12 23.09919361243014 25.113558492155697 30.70010513068233 21.087142764731837 13 27.968770457711134 22.22754469607301 26.042478950083314 23.761205896132537 14 21.778755433611387 21.235157765267985 27.75801441253922 29.2280723885814 15 23.201820669708823 29.057831801395245 24.46585350986529 23.274494019030644 16 22.856785488302478 28.425853323214888 25.86698731761051 22.850373870872126 17 21.81620981951196 27.90268243889802 27.31371981290663 22.967387928683394 18 21.743836850941516 27.371029061320147 28.593018031628258 22.292116056110075 19 22.690441774158547 26.953598922217548 28.81444455478684 21.54151474883707 20 22.807520854482057 26.475051334918042 28.844864511545893 21.872563299054008 21 23.028906740211404 26.231161430212552 28.707568220348335 22.032363609227705 22 22.548370519598848 26.218707996649716 28.750074876965325 22.482846606786115 23 22.559679941486543 26.144207083827254 28.624565983168974 22.67154699151723 24 22.181059384312547 26.29790369051992 28.84292313601971 22.67811378914783 25 22.564410556642354 26.15452438413719 28.74970509310125 22.531359966119204 26 22.240733246792836 26.413281689326613 28.87238079221346 22.473604271667096 27 22.364299858319427 26.46089700200539 28.59267465178951 22.58212848788568 28 22.386800693328343 26.374332135390333 28.641576785903865 22.59729038537746 29 22.514624647623393 26.52510803237318 28.517287545093428 22.442979774909997 30 22.44949549363001 26.518708964021044 28.723726856535613 22.308068685813332 31 22.536701842002106 26.163113926258962 28.70347307830952 22.59671115342941 32 22.278150859149683 26.558478031167198 28.59345141047877 22.56991969920435 33 22.5391624364282 26.36090550310194 28.446381103457767 22.65355095701209 34 22.633956978026625 26.53240624706082 28.51699299115632 22.316643783756234 35 22.733539289241953 26.407330349074247 28.37441943551268 22.48471092617111 36 22.572468908119355 26.46311796262144 28.45105426038892 22.513358868870288 37 22.619781314477475 26.23879500714818 28.404686433584953 22.736737244789392 38 22.499879162840156 26.395879347773548 28.471861753883076 22.632379735503218 39 22.730446688884555 26.44121620095089 28.28882487044672 22.539512239717833 40 22.672704860925023 26.235827950345154 28.633924227282424 22.457542961447402 41 22.470451315274417 26.428858536739146 28.310096336987677 22.790593810998757 42 22.690885858337484 26.34851130992537 28.320734057555256 22.63986877418189 43 22.591175696358963 26.233947607765995 28.336803765826456 22.838072930048586 44 22.630237751215027 26.462892420859056 28.210009194798367 22.696860633127542 45 22.806038654195945 26.446169834259603 27.919055890230744 22.828735621313715 46 22.816396009225645 26.41413478057571 27.89339021430185 22.876078995896794 47 22.739175298896882 26.548494955162838 27.72674446707143 22.985585278868847 48 22.563613809756735 26.718141606171532 27.75070521472731 22.967539369344426 49 22.765500929388537 26.58271873203874 27.5944860833392 23.057294255233522 50 22.897182022693862 26.601090036786378 27.64541967046719 22.85630827005257 51 22.684029353911335 26.840030850187457 27.415056908928904 23.060882886972312 52 22.80717147172785 26.775648519077954 27.418086294082876 22.999093715111314 53 22.71848475487384 26.79954271976665 27.51572426170047 22.96624826365904 54 22.645312901414133 26.912563497313002 27.635102370618892 22.807021230653973 55 22.73995085690449 26.65768634474454 27.499486450974324 23.102876347376643 56 22.633657935304484 26.993139118583226 27.473526917584305 22.899676028527985 57 22.735437718265445 26.71046292370488 27.391259347290763 23.162840010738908 58 22.591283058915003 26.57211755681878 27.628479263641236 23.20812012062498 59 22.691867359575387 26.776865843771887 27.50692094336044 23.02434585329229 60 22.59771986970684 26.81527792371546 27.743143847851215 22.84385835872649 61 22.75179808869345 26.611712663062608 27.722350938578654 22.914138309665287 62 22.561854223617956 26.82283537806197 27.603784035456673 23.011526362863396 63 22.468790063260546 26.683182362707804 27.74200836468338 23.10601920934827 64 22.69803297655408 26.61657991068872 27.58262134233837 23.102765770418827 65 22.62314567502395 26.606118280592582 27.62274894366993 23.14798710071354 66 22.57284627102444 26.837137423853818 27.48603187564599 23.10398442947575 67 22.67929374401636 26.61433467184697 27.418073402112125 23.288298182024544 68 22.558991380981833 26.77581042048387 27.638342781867852 23.026855416666447 69 22.6370068674845 26.62520221276855 27.442617283405756 23.295173636341186 70 22.476359921896837 26.55496961837484 27.613544887248544 23.355125572479775 71 22.560655878962503 26.654540219558047 27.44402026398666 23.340783637492788 72 22.474914665725045 26.869209459913908 27.30008449354318 23.355791380817863 73 22.617720337816465 26.643713333074132 27.393313234717713 23.345253094391687 74 22.511837508098793 26.811121661365554 27.273114209160926 23.403926621374723 75 22.586283619391164 26.78570959486772 27.304990062535367 23.323016723205754 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 854.0 1 663.5 2 552.0 3 638.5 4 646.0 5 507.5 6 479.5 7 961.5 8 1333.0 9 2286.0 10 3706.5 11 3901.5 12 3629.0 13 3177.5 14 2730.0 15 3019.0 16 3304.0 17 3646.5 18 4296.5 19 5237.0 20 5870.0 21 6544.0 22 8391.0 23 10820.5 24 12077.0 25 13671.5 26 17068.5 27 20901.5 28 22932.0 29 25163.5 30 29763.5 31 35328.5 32 38525.0 33 41455.5 34 47258.5 35 53411.5 36 56692.0 37 59122.5 38 63708.0 39 67896.0 40 69929.0 41 72783.5 42 77877.0 43 81175.0 44 82234.0 45 84820.5 46 88660.0 47 91583.5 48 93254.0 49 94179.0 50 94800.0 51 93008.0 52 91520.0 53 89597.0 54 85423.5 55 78961.0 56 74749.0 57 71330.5 58 61967.5 59 51001.5 60 45980.0 61 41970.5 62 33001.5 63 24164.0 64 20286.0 65 16737.5 66 11038.0 67 7789.0 68 6691.0 69 5563.5 70 3592.5 71 2135.5 72 1522.0 73 1287.0 74 921.5 75 600.5 76 410.0 77 326.5 78 190.5 79 99.0 80 60.0 81 53.0 82 31.0 83 11.0 84 6.0 85 5.5 86 3.0 87 1.0 88 1.0 89 1.0 90 1.5 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07076705817083703 2 0.014237470500591563 3 0.002259082035149215 4 0.14253231538046093 5 0.09330534173081409 6 0.0688231968847784 7 0.0291579192908794 8 0.02637346934057921 9 0.04996248873085822 10 0.028474941001183127 11 0.016076258203619995 12 0.10428553115746958 13 0.08610780129324566 14 0.04822877461085998 15 0.020594422273918424 16 0.033203252237541954 17 0.022117989227856266 18 0.02012159115028254 19 0.013712102585440583 20 0.012608829963623527 21 0.028895235333303914 22 0.016076258203619995 23 0.014500154458167054 24 0.021907842061795874 25 0.014972985581802937 26 0.0345692088169345 27 0.0292104560823945 28 0.037931563473900774 29 0.05195888680843195 30 0.035567407855721364 31 0.02048934869088823 32 0.010980189426655487 33 0.013922249751500975 34 0.013712102585440583 35 0.005673973483630587 36 0.01040228471998941 37 0.007722908352719409 38 0.002101471660603921 39 0.00824827626787039 40 0.011032726218170585 41 0.011190336592715878 42 0.007565297978174116 43 0.01087511584362529 44 0.009351548889687448 45 0.004728311236358822 46 0.0019963980775737247 47 0.00504353198544941 48 0.005621436692115489 49 0.0013659565793925485 50 3.152207490905881E-4 51 0.001891324494543529 52 0.0027844499503001953 53 0.0 54 5.253679151509802E-5 55 3.6775754060568616E-4 56 0.009141401723627056 57 0.0036250386145417635 58 4.2029433212078415E-4 59 0.007722908352719409 60 0.0014710301624227446 61 9.456622472717645E-4 62 0.02553288067633764 63 0.009876916804838428 64 0.01034974792847431 65 0.019806370401191955 66 0.018125193072708816 67 0.016916846867861563 68 0.016338942161195486 69 0.005831583858175881 70 0.015971184620589798 71 0.007880518727264703 72 0.01702192045089176 73 0.007670371561204311 74 0.019281002486040977 75 0.0013134197878774507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 1903428.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.28534551025494 #Duplication Level Percentage of deduplicated Percentage of total 1 81.1012914213322 56.19130997454877 2 11.667982529438586 16.168404019195417 3 3.5312733613636356 7.339964847997166 4 1.4400890442129457 3.9910826797530703 5 0.7063525219233049 2.446993926674806 6 0.40140052765698625 1.6686704548027766 7 0.25274238798324883 1.2257940573554413 8 0.18514719261770068 1.0262389768616895 9 0.11889830551807973 0.7414119160563605 >10 0.5601532185993718 6.645240050530339 >50 0.024709115786228246 1.1552157500167062 >100 0.009656484117080104 1.0568965529037178 >500 7.59723626573069E-5 0.03642529805991795 >1k 2.2791708797192072E-4 0.3063514952438186 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2530 0.132918082533198 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.2536791515098025E-5 2 0.0 0.0 0.0 0.0 5.2536791515098025E-5 3 0.0 0.0 0.0 0.0 5.2536791515098025E-5 4 0.0 0.0 0.0 0.0 5.2536791515098025E-5 5 0.0 0.0 0.0 0.0 5.2536791515098025E-5 6 0.0 0.0 0.0 0.0 5.2536791515098025E-5 7 0.0 0.0 0.0 5.2536791515098025E-5 5.2536791515098025E-5 8 0.0 0.0 0.0 5.2536791515098025E-5 5.2536791515098025E-5 9 0.0 0.0 0.0 5.2536791515098025E-5 5.2536791515098025E-5 10 0.0 0.0 0.0 5.2536791515098025E-5 5.2536791515098025E-5 11 0.0 0.0 0.0 5.2536791515098025E-5 5.2536791515098025E-5 12 0.0 0.0 0.0 5.2536791515098025E-5 1.5761037454529406E-4 13 0.0 0.0 0.0 5.2536791515098025E-5 2.101471660603921E-4 14 0.0 0.0 0.0 5.2536791515098025E-5 2.101471660603921E-4 15 0.0 0.0 0.0 5.2536791515098025E-5 2.101471660603921E-4 16 0.0 0.0 0.0 5.2536791515098025E-5 2.6268395757549014E-4 17 0.0 0.0 0.0 5.2536791515098025E-5 2.6268395757549014E-4 18 0.0 0.0 0.0 5.2536791515098025E-5 2.6268395757549014E-4 19 0.0 0.0 0.0 5.2536791515098025E-5 3.152207490905881E-4 20 0.0 0.0 0.0 5.2536791515098025E-5 3.152207490905881E-4 21 0.0 0.0 0.0 5.2536791515098025E-5 3.152207490905881E-4 22 0.0 0.0 0.0 5.2536791515098025E-5 3.152207490905881E-4 23 0.0 0.0 0.0 5.2536791515098025E-5 3.152207490905881E-4 24 0.0 0.0 0.0 1.5761037454529406E-4 3.152207490905881E-4 25 0.0 0.0 0.0 2.6268395757549014E-4 3.152207490905881E-4 26 0.0 0.0 0.0 3.6775754060568616E-4 3.152207490905881E-4 27 0.0 0.0 0.0 4.202943321207842E-4 3.6775754060568616E-4 28 0.0 0.0 0.0 8.405886642415684E-4 3.6775754060568616E-4 29 0.0 0.0 0.0 0.0017337141199982348 3.6775754060568616E-4 30 0.0 0.0 0.0 0.0037826489890870575 3.6775754060568616E-4 31 0.0 0.0 0.0 0.008931254557566664 3.6775754060568616E-4 32 0.0 0.0 0.0 0.013607029002410388 3.6775754060568616E-4 33 0.0 0.0 0.0 0.019753833609676856 3.6775754060568616E-4 34 0.0 0.0 0.0 0.028685088167243522 3.6775754060568616E-4 35 0.0 0.0 0.0 0.03850946818056685 3.6775754060568616E-4 36 0.0 0.0 0.0 0.05258932830661312 3.6775754060568616E-4 37 0.0 0.0 0.0 0.06750977709690097 3.6775754060568616E-4 38 0.0 0.0 0.0 0.0875262946641533 3.6775754060568616E-4 39 0.0 0.0 0.0 0.10775295939746604 3.6775754060568616E-4 40 0.0 0.0 0.0 0.12845245525441468 3.6775754060568616E-4 41 0.0 0.0 0.0 0.14752331057439524 3.6775754060568616E-4 42 0.0 0.0 0.0 0.16838041680588917 3.6775754060568616E-4 43 0.0 0.0 0.0 0.18929005982889818 3.6775754060568616E-4 44 0.0 0.0 0.0 0.21613636029311326 4.202943321207842E-4 45 0.0 0.0 0.0 0.24750082482762678 4.728311236358822E-4 46 0.0 0.0 0.0 0.2789703629451705 4.728311236358822E-4 47 0.0 0.0 0.0 0.3138547925111956 4.728311236358822E-4 48 0.0 0.0 0.0 0.3474258022893432 4.728311236358822E-4 49 0.0 0.0 0.0 0.38362365164324574 4.728311236358822E-4 50 0.0 0.0 0.0 0.4219755094492673 4.728311236358822E-4 51 0.0 0.0 0.0 0.4600646832977134 4.728311236358822E-4 52 0.0 0.0 0.0 0.4945288185316177 5.253679151509803E-4 53 0.0 0.0 0.0 0.5305690575109749 5.253679151509803E-4 54 0.0 0.0 0.0 0.5713901445182061 5.253679151509803E-4 55 0.0 5.2536791515098025E-5 0.0 0.6184105729242189 5.253679151509803E-4 56 0.0 5.2536791515098025E-5 0.0 0.6718930266865886 5.253679151509803E-4 57 0.0 5.2536791515098025E-5 0.0 0.727319341735017 5.253679151509803E-4 58 0.0 5.2536791515098025E-5 0.0 0.7870011368961684 5.253679151509803E-4 59 0.0 5.2536791515098025E-5 0.0 0.8497300659651954 5.253679151509803E-4 60 0.0 5.2536791515098025E-5 0.0 0.9157688128996736 5.253679151509803E-4 61 0.0 5.2536791515098025E-5 0.0 0.9803365296717291 5.253679151509803E-4 62 0.0 5.2536791515098025E-5 0.0 1.0439060474049977 6.304414981811762E-4 63 0.0 5.2536791515098025E-5 0.0 1.1088415217176588 6.304414981811762E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1945 0.0 40.732147 1 GTATCAA 4405 0.0 37.619328 1 GCGTATC 100 4.900576E-7 27.676514 1 TATCAAC 6040 0.0 27.133114 2 TCAACGC 6150 0.0 26.64921 4 ATCAACG 6175 0.0 26.542017 3 CAACGCA 6260 0.0 26.143745 5 AACGCAG 6480 0.0 25.197563 6 ACGCAGA 7405 0.0 21.816908 7 CGCAGAG 7485 0.0 21.445936 8 GAGTACT 5775 0.0 20.206648 12 GTACATG 4410 0.0 20.16118 1 AGAGTAC 7515 0.0 20.076221 11 AGTACTT 6200 0.0 18.980581 13 TACATGG 4525 0.0 18.94922 2 CAGAGTA 8025 0.0 18.885399 10 GTACTTT 6250 0.0 18.711416 14 TATACTG 780 0.0 18.591675 5 GCAGAGT 8480 0.0 18.441559 9 CCTATAC 455 0.0 18.238638 3 >>END_MODULE