FastQCFastQC Report
Mon 6 Feb 2017
SRR2077509_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077509_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3715785
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63950.038.0591051
GGTATCA28350.036.410621
TCAACGC82000.029.1176344
ATCAACG82450.028.9579323
TATCAAC82800.028.9184762
CAACGCA84700.028.2286575
AACGCAG87800.027.1673896
ACGCAGA99750.023.6049277
CGCAGAG104750.022.4138898
AGAGTAC101300.022.05679511
TATACCG2057.4578566E-1121.879125
CGAATCG3000.021.855237
GAGTACT62450.020.98108712
GTACATG94000.020.5302351
CAGAGTA112200.020.28225510
AGTACTT67400.019.95419713
GCAGAGT117950.019.556079
GTACTTT66300.019.50532214
TACATGG97200.019.3453462
ATACCGT3500.018.7120726
Produced by FastQC (version 0.11.5)