FastQCFastQC Report
Mon 6 Feb 2017
SRR2077509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3715785
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36400.040.5905531
GTATCAA80100.037.4096681
TATCAAC114200.026.0735992
ATCAACG115600.025.5810933
TCAACGC116250.025.5567234
CAACGCA118400.025.1127645
AACGCAG123600.024.190726
CGAATCG3650.023.6213837
ACGCAGA142250.020.7513187
CGCAGAG148050.019.8922638
GAGTACT105450.019.415912
AGAGTAC143500.019.36838311
GTACATG89600.018.9614681
AGTACTT110600.018.87827913
CAGAGTA153550.018.39276710
GTACTTT111900.018.21957614
TACATGG92200.017.8166732
GCAGAGT161300.017.7873089
ACATGGG95050.016.73853
TACTTTT124200.016.49743715
Produced by FastQC (version 0.11.5)