FastQCFastQC Report
Mon 6 Feb 2017
SRR2077508_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077508_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2928960
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34250.040.820811
GTATCAA70250.035.921811
TATCAAC92700.027.0557352
TCAACGC93050.026.4362664
ATCAACG93650.026.376963
CAACGCA95600.025.5871985
AACGCAG97950.024.9854496
GAGTACT65850.021.7815912
ACGCAGA112050.021.5670817
CGCAGAG113500.021.2326228
GTACATG98450.021.1440771
GTACTTT68050.020.77767614
AGAGTAC110700.020.68104611
TATACCG1851.1777956E-820.5142355
GTGGTAT16150.020.3066481
AGTACTT70900.020.2812113
TACATGG102000.020.0566882
CAGAGTA116250.019.95994410
GCAGAGT123200.019.0854979
GCGATAC2204.2164174E-918.81047414
Produced by FastQC (version 0.11.5)