FastQCFastQC Report
Mon 6 Feb 2017
SRR2077508_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077508_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2928960
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49240.16811427947121163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31040.10597618267234787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30940.1056347645580684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39200.044.735451
GTATCAA88300.037.996271
TATCAAC121300.027.3320452
TCAACGC122950.026.9122314
ATCAACG124000.026.7122333
CAACGCA126200.026.1868925
AACGCAG130950.025.2326936
GTGGTAT14450.023.218481
ACGCAGA150800.021.7742777
CGCAGAG154650.021.1439888
AGAGTAC151700.020.53159111
GAGTACT107650.020.11199812
CAGAGTA156900.020.0712110
GTACATG96300.020.0418511
GCAGAGT163600.019.5232759
TACATGG97400.019.5217172
AGTACTT112550.019.40985913
GTACTTT112900.019.1896314
TACTTTT119650.018.04723415
ACATGGG102800.017.7249013
Produced by FastQC (version 0.11.5)