FastQCFastQC Report
Mon 6 Feb 2017
SRR2077507_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077507_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122653
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30750.035.376211
GTATCAA59550.032.59121
TCAACGC74300.025.4040284
ATCAACG74600.025.347313
TATCAAC75400.025.1686842
CAACGCA75500.025.0443525
AACGCAG79800.023.7160026
ACGCAGA90750.020.551837
CGCAGAG93300.019.9920488
GAGTACT52750.019.41348612
AGAGTAC89600.019.35661911
GTATAGC10550.019.3128071
GTACATG80500.018.5753881
TACATGG79750.018.4742322
CAGAGTA98950.017.90972710
AGTACTT60100.017.50050713
GTACTTT58700.017.27222814
GCAGAGT105500.017.1246039
ACATGGG83400.016.8804593
GTGTAGC13300.016.8774781
Produced by FastQC (version 0.11.5)