FastQCFastQC Report
Mon 6 Feb 2017
SRR2077507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122653
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34550.039.6615261
GTATCAA68250.034.1220131
ATCAACG95400.024.1148153
TCAACGC95450.024.102184
TATCAAC96100.024.0448382
CAACGCA97750.023.5300145
AACGCAG102300.022.5455636
ACGCAGA117600.019.4346247
CGCAGAG121550.018.7471528
GAGTACT85550.018.7271712
AGAGTAC118200.018.40336611
GTACATG73950.017.9218541
GTACTTT91250.017.46600314
CAGAGTA126900.017.3865510
AGTACTT93300.017.38471813
TACATGG75550.017.1698132
GTGGTAT16550.016.935941
GCAGAGT132150.016.7997999
ACACCGT4250.016.2480556
TATACCG1756.306653E-515.7876215
Produced by FastQC (version 0.11.5)