FastQCFastQC Report
Mon 6 Feb 2017
SRR2077506_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077506_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3945811
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34300.036.440831
GTATCAA64950.033.8105131
TCAACGC83200.025.8773924
ATCAACG85050.025.3534683
CAACGCA85100.025.3401785
TATCAAC85700.025.1598932
AACGCAG89400.023.9836396
CGTGCCG6000.022.98664910
ACGCAGA103900.020.5066997
TATACCG2102.3519533E-919.7161065
CGCAGAG109300.019.4006568
GCACCGT8700.019.0188476
TCAATCG4550.018.95996545
AGAGTAC105950.018.64797211
GAGTACT55600.018.60524712
GTGGTAT18150.018.0726071
CCGGTAT5100.017.59455558
TGCGATA4750.017.42500113
CAGAGTA116550.017.42488510
GTACATG107350.017.3686281
Produced by FastQC (version 0.11.5)