FastQCFastQC Report
Mon 6 Feb 2017
SRR2077506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3945811
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39150.038.4405061
GTATCAA74650.032.5804521
TATCAAC102000.023.726982
TCAACGC103200.023.3864574
ATCAACG103700.023.2731063
CAACGCA103700.023.2420965
CGTGCCG5250.023.0189410
AACGCAG110450.021.9129756
CGAATCG4600.020.9925237
ACGCAGA128300.018.8645277
GTACATG105150.018.6883371
GCGATAC4900.018.30540314
CGCAGAG133800.017.8830978
TACATGG108050.017.854762
GCACCGT7950.017.8059756
GGACCGT3750.017.4932846
GAGTACT81150.017.4024912
TATACCG2005.94282E-717.2650815
AGAGTAC128650.017.23065811
CGTACAA4700.016.88252825
Produced by FastQC (version 0.11.5)