FastQCFastQC Report
Mon 6 Feb 2017
SRR2077505_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077505_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895339
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27800.035.52271
GTATCAA56250.034.8666761
TATCAAC71650.027.1072792
ATCAACG72150.026.7300193
TCAACGC72350.026.7038124
CAACGCA74200.026.1323665
AACGCAG77700.025.0228186
GTGGTAT15550.022.4272351
ACGCAGA90750.020.8554177
TGGTATC15800.020.3057752
CGCAGAG93700.019.9804828
AGAGTAC89250.019.66392911
GAGTACT53250.019.36031212
GTACATG82500.018.6247651
CAGAGTA96350.018.53665210
AGTACTT57950.017.8505313
TACATGG83350.017.79742
GCAGAGT102450.017.7358469
GTACTTT58250.017.69970714
GTGTAGC14550.017.0865461
Produced by FastQC (version 0.11.5)