FastQCFastQC Report
Mon 6 Feb 2017
SRR2077505_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077505_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895339
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30100.041.3757971
GTATCAA62450.034.234691
TATCAAC83700.025.443232
TCAACGC84350.025.2096734
ATCAACG84850.025.143493
CAACGCA86650.024.4675675
AACGCAG90000.023.5539786
GTGGTAT15100.020.1611861
ACGCAGA106650.019.7817087
CGCAGAG110100.019.1001158
GTACATG77550.018.9590911
TGGTATC15900.018.4815672
AGAGTAC108800.018.3444611
TACATGG77200.018.2708972
GAGTACT76600.017.98664312
CAGAGTA114700.017.61158610
ACATGGG81200.017.3312783
GCAGAGT119400.017.3232159
AGTACTT80700.017.15039413
GTACTTT80550.017.04435714
Produced by FastQC (version 0.11.5)